pyTRACTnmr: an open source python package for analyzing [15N, 1H]-TRACT experiments [0.03%]
pyTRACTnmr：一个用于分析[15N, 1H]-TRACT实验的开源Python软件包
Debadutta Patra,Mandar V Deshmukh
Debadutta Patra
The rotational correlation time ([Formula: see text]) is a one of the fundamental parameters for characterizing biomacromolecules in solution. The [15N, 1H]-TRACT (TROSY for Rotational Correlation Times) NMR experiment is a widely utilized ...
RelCalc: symbolic evaluation of BWR theory relaxation rates in python, applications to TROSY effects in AX[Formula: see text] spin systems [0.03%]
RelCalc：用python计算BWR理论弛豫率的符号求解及其在AX双量子系统TROSY效应中的应用
James Eaton,Daniel Stedman,Andrew J Baldwin
James Eaton
Relaxation phenomena greatly influence magnetic resonance experiments, from setting repetition rates and measurement times through to measuring internal motion of molecules. RelCalc provides a convenient and efficient means for symbolic and...
Solution NMR study of the titin I-band IgI domain I82 shows unusual conformational dynamics [0.03%]
溶液NMR研究显示巨肌蛋白I带IgI结构域I82具有异常的构象动态变化
Colleen M Kelly,Shayma Abukar,Matthew J Gage et al.
Colleen M Kelly et al.
β-sandwich domains of the intermediate immunoglobulin fold (IgI) are key building blocks of many large, multidomain proteins - such as titin. The uniformity of the basic fold of these domains does not seem to be an impediment to their adap...
Methyl-specific NMR of therapeutic antibodies: cost-effective isotopic labeling strategies in CHO cells for high-resolution structural characterization [0.03%]
治疗性抗体的甲基专用NMR：CHO细胞中用于高分辨率结构表征的经济型同位素标记策略
Rida Awad,Arthur Giraud,Béatrice Vibert et al.
Rida Awad et al.
Monoclonal antibodies (mAbs) are essential therapeutic agents whose efficacy depends critically on the integrity of their higher-order structure (HOS). Subtle perturbations in HOS, arising from post-translational modifications, chemical deg...
Thorben Maass,Lorena Rudolph,Thomas Peters et al.
Thorben Maass et al.
Over the last 20 years, the number of large proteins accessible to protein-NMR analysis has increased significantly due to the development of selective [1H,13C]-methyl labelling techniques in combination with methyl-TROSY based NMR experime...
Olivia Gampp,Riccardo Cadalbert,Roland Riek et al.
Olivia Gampp et al.
Solid-state NMR spectroscopy is often limited by low spectral resolution, a problem typically addressed using fast magic-angle spinning (MAS) and ¹H detection, which require costly specialized hardware. Here, we demonstrate that the super-...
Optimus peak: automatic peak information extraction from 2D and 3D solution and solid state spectra using evolutionary algorithms [0.03%]
基于进化算法的二维和三维溶液及固态谱峰信息自动提取方法优化研究
Rachel E Brown,Allan R Willms,Steffen P Graether
Rachel E Brown
Cupriavidus necator, an alternative source for isotopic enrichment of proteins expressed in insect cells for NMR investigations [0.03%]
利用昆虫细胞表达蛋白质的 Cupriavidus necator 的同位素丰富来源用于核磁共振研究
Santosh L Gande,Sridhar Sreeramulu,Krishna Saxena et al.
Santosh L Gande et al.
Isotopic enrichment of pharmacologically relevant protein targets is crucial for structural studies by nuclear magnetic resonance (NMR) and plays a key role in advancing structure-guided drug discovery. Many clinically important drug target...
M9 medium composition alters E. coli metabolism during recombinant expression [0.03%]
M9培养基组成成分的改变会影响大肠杆菌的代谢及重组蛋白表达
Çağdaş Dağ,Oktay Gocenler,Nilufer Cakir et al.
Çağdaş Dağ et al.
M9 minimal media and its enhanced variants (M9 + and M9++) are widely used for recombinant protein expression in Escherichia coli, particularly for isotopic labeling required in structural biology techniques such as NMR spectroscopy. This s...
Web server DDfit: a new scheme to process PFG NMR diffusion data with improved precision [0.03%]
一种提高PFG-NMR扩散数据处理精度的新方案DDfit及其网络服务器发布
Vladislav A Salikov,Olga O Lebedenko,Nikolai R Skrynnikov et al.
Vladislav A Salikov et al.
In this communication we describe a new scheme to process the data from stimulated echo protein diffusion experiments. For a series of gradient-encoded proton spectra [Formula: see text] considered over the selected spectral region [Formula...