Thermal stability of chicken brain α-spectrin repeat 17: a spectroscopic study [0.03%]
鸡脑α-光谱蛋白第17重复序列热稳定性的光谱学研究
Annette K Brenner,Bruno Kieffer,Gilles Travé et al.
Annette K Brenner et al.
Spectrin is a rod-like multi-modular protein that is mainly composed of triple-helical repeats. These repeats show very similar 3D-structures but variable conformational and thermodynamical stabilities, which may be of great importance for ...
Properties of the DREAM scheme and its optimization for application to proteins [0.03%]
DREAM方案的性质及其在蛋白质应用中的优化
Thomas Westfeld,René Verel,Matthias Ernst et al.
Thomas Westfeld et al.
The DREAM scheme is an efficient adiabatic homonuclear polarization-transfer method suitable for multi-dimensional experiments in biomolecular solid-state NMR. The bandwidth and dynamics of the polarization transfer in the DREAM experiment ...
Increased precision for analysis of protein-ligand dissociation constants determined from chemical shift titrations [0.03%]
基于化学位移滴定测定的蛋白质-配体解离常数分析精度提高
Craig J Markin,Leo Spyracopoulos
Craig J Markin
NMR is ideally suited for the analysis of protein-protein and protein ligand interactions with dissociation constants ranging from ~2 μM to ~1 mM, and with kinetics in the fast exchange regime on the NMR timescale. For the determination of...
Selective editing of Val and Leu methyl groups in high molecular weight protein NMR [0.03%]
高分子量蛋白质NMR中Val和Leu甲基的选择性编辑
Weidong Hu,Andrew T Namanja,Steven Wong et al.
Weidong Hu et al.
The development of methyl-TROSY approaches and specific (13)C-(1)H labeling of Ile, Leu and Val methyl groups in highly deuterated proteins has made it possible to study high molecular weight proteins, either alone or in complexes, using so...
A strong 13C chemical shift signature provides the coordination mode of histidines in zinc-binding proteins [0.03%]
一个强碳-13化学变化标志提供了锌结合蛋白组胺酸配位模式
Pierre Barraud,Mario Schubert,Frédéric H-T Allain
Pierre Barraud
Zinc is the second most abundant metal ion incorporated in proteins, and is in many cases a crucial component of protein three-dimensional structures. Zinc ions are frequently coordinated by cysteine and histidine residues. Whereas cysteine...
Probing water-protein contacts in a MMP-12/CGS27023A complex by nuclear magnetic resonance spectroscopy [0.03%]
核磁共振技术研究基质金属蛋白酶与小分子结合复合物中的水分子作用关系
Helena Kovacs,Tatiana Agback,Johan Isaksson
Helena Kovacs
Using the case of the catalytic domain of MMP-12 in complex with the known inhibitor CGS27023A, a recently assembled 3D (15)N-edited/(14)N,(12)C-filtered ROESY experiment is used to monitor and distinguish protein amide protons in fast exch...
Yingang Feng,Yong Geng,Tao Zhou et al.
Yingang Feng et al.
Convenient method for resolving degeneracies due to symmetry of the magnetic susceptibility tensor and its application to pseudo contact shift-based protein-protein complex structure determination [0.03%]
磁化率张量对称性简并解决的简便方法及其在伪接触移位蛋白质-蛋白质复合物结构测定中的应用
Yoshihiro Kobashigawa,Tomohide Saio,Masahiro Ushio et al.
Yoshihiro Kobashigawa et al.
Pseudo contact shifts (PCSs) induced by paramagnetic lanthanide ions fixed in a protein frame provide long-range distance and angular information, and are valuable for the structure determination of protein-protein and protein-ligand comple...
Measurement of the signs of methyl 13C chemical shift differences between interconverting ground and excited protein states by R(1ρ): an application to αB-crystallin [0.03%]
利用R(1rho)测量蛋白基态和激发态之间互变的甲基碳化学位移差符号——以αB-晶状体蛋白质为例
Andrew J Baldwin,Lewis E Kay
Andrew J Baldwin
Carr-Purcell-Meiboom-Gill relaxation dispersion (CPMG RD) NMR spectroscopy has emerged as a powerful tool for quantifying the kinetics and thermodynamics of millisecond time-scale exchange processes involving the interconversion between a v...
Quantitative comparison of errors in 15N transverse relaxation rates measured using various CPMG phasing schemes [0.03%]
不同CPMG相位方案测量15N横向弛豫速率的误差量化比较研究
Wazo Myint,Yufeng Cai,Celia A Schiffer et al.
Wazo Myint et al.
Nitrogen-15 Carr-Purcell-Meiboom-Gill (CPMG) transverse relaxation experiment are widely used to characterize protein backbone dynamics and chemical exchange parameters. Although an accurate value of the transverse relaxation rate, R(2), is...