NMR structure note: a defective isoform and its activity-improved variant of a type III antifreeze protein from Zoarces elongates Kner [0.03%]
第三类鱼类结冰蛋白elongatic的变化型及其改进型的核磁共振结构研究
Hiroyuki Kumeta,Kenji Ogura,Yoshiyuki Nishimiya et al.
Hiroyuki Kumeta et al.
Exploring signal-to-noise ratio and sensitivity in non-uniformly sampled multi-dimensional NMR spectra [0.03%]
非均匀采样多维核磁共振谱的信噪比和灵敏度研究
Sven G Hyberts,Scott A Robson,Gerhard Wagner
Sven G Hyberts
It is well established that non-uniform sampling (NUS) allows acquisition of multi-dimensional NMR spectra at a resolution that cannot be obtained with traditional uniform acquisition through the indirect dimensions. However, the impact of ...
Comparative Study
Journal of biomolecular NMR. 2013 Feb;55(2):167-78. DOI:10.1007/s10858-012-9698-2 2013
Secondary structural analysis of proteins based on (13)C chemical shift assignments in unresolved solid-state NMR spectra enhanced by fragmented structure database [0.03%]
基于无解析固态NMR光谱中(13)C化学位移指派的片段化结构数据库增强的蛋白质二级结构分析
Keisuke Ikeda,Ayako Egawa,Toshimichi Fujiwara
Keisuke Ikeda
Magic-angle-spinning solid-state (13)C NMR spectroscopy is useful for structural analysis of non-crystalline proteins. However, the signal assignments and structural analysis are often hampered by the signal overlaps primarily due to minor ...
A systematic study of labelling an α-helix in a protein with a lanthanide using IDA-SH or NTA-SH tags [0.03%]
利用IDA-SH或NTA-SH标签对蛋白质中的α-螺旋进行镧系金属标记的系统研究
Hiromasa Yagi,Ansis Maleckis,Gottfried Otting
Hiromasa Yagi
The previously published IDA-SH and NTA-SH tags are small synthetic lanthanide-binding tags derived from cysteine, which afford site-specific lanthanide labelling by disulfide-bond formation with a cysteine residue of the target protein. Fo...
Solution structure of the two RNA recognition motifs of hnRNP A1 using segmental isotope labeling: how the relative orientation between RRMs influences the nucleic acid binding topology [0.03%]
利用片段式同位素标记测定hnRNP A1的两个RNA识别模体的溶液结构：RRM之间的相对取向如何影响结合核酸时的构象拓扑关系
Pierre Barraud,Frédéric H-T Allain
Pierre Barraud
Human hnRNP A1 is a multi-functional protein involved in many aspects of nucleic-acid processing such as alternative splicing, micro-RNA biogenesis, nucleo-cytoplasmic mRNA transport and telomere biogenesis and maintenance. The N-terminal r...
HASH: a program to accurately predict protein Hα shifts from neighboring backbone shifts [0.03%]
利用邻近主链移位准确预测蛋白质Hα移位的程序HASH
Jianyang Zeng,Pei Zhou,Bruce Randall Donald
Jianyang Zeng
Chemical shifts provide not only peak identities for analyzing nuclear magnetic resonance (NMR) data, but also an important source of conformational information for studying protein structures. Current structural studies requiring H(α) che...
Biophysical and structural investigation of bacterially expressed and engineered CCR5, a G protein-coupled receptor [0.03%]
用于生物物理及结构研究的细菌表达并改造过的CCR5（G蛋白偶联受体）
Maciej Wiktor,Sébastien Morin,Hans-Jürgen Sass et al.
Maciej Wiktor et al.
The chemokine receptor CCR5 belongs to the class of G protein-coupled receptors. Besides its role in leukocyte trafficking, it is also the major HIV-1 coreceptor and hence a target for HIV-1 entry inhibitors. Here, we report Escherichia col...
Residue-specific membrane location of peptides and proteins using specifically and extensively deuterated lipids and ¹³C-²H rotational-echo double-resonance solid-state NMR [0.03%]
基于特异性氘代脂质和13C-2H回波双重共振固态NMR的多肽及蛋白质残基定位研究
Li Xie,Ujjayini Ghosh,Scott D Schmick et al.
Li Xie et al.
Residue-specific location of peptides in the hydrophobic core of membranes was examined using (13)C-(2)H REDOR and samples in which the lipids were selectively deuterated. The transmembrane topology of the KALP peptide was validated with th...
Utilization of lysine ¹³C-methylation NMR for protein-protein interaction studies [0.03%]
利用lysine甲基化核磁共振研究蛋白质-蛋白质相互作用
Yoshikazu Hattori,Kyoko Furuita,Izuru Ohki et al.
Yoshikazu Hattori et al.
Chemical modification is an easy way for stable isotope labeling of non-labeled proteins. The reductive (13)C-methylation of the amino group of the lysine side-chain by (13)C-formaldehyde is a post-modification and is applicable to most pro...
Structural characterization of supramolecular assemblies by ¹³C spin dilution and 3D solid-state NMR [0.03%]
利用13C自旋稀释和固态三维NMR表征超分子组装体的结构特征
Birgit Habenstein,Antoine Loquet,Karin Giller et al.
Birgit Habenstein et al.
(13)C spin diluted protein samples can be produced using [1-(13)C] and [2-(13)C]-glucose (Glc) carbon sources in the bacterial growth medium. The (13)C spin dilution results in favorable (13)C spectral resolution and polarization transfer b...