Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications [0.03%]
玻璃NMR管产生的微摩尔级氟污染及生物分子应用的简易解决方案
Khushboo Matwani,Jasmine Cornish,Erika Alden DeBenedictis et al.
Khushboo Matwani et al.
Fluorine (19F) NMR is emerging as an invaluable analytical technique in chemistry, biochemistry, structural biology, material science, drug discovery, and medicine, especially due to the inherent rarity of naturally occurring fluorine in bi...
Quantitative analysis of the slow exchange process by 19F NMR in the presence of scalar and dipolar couplings: applications to the ribose 2'-19F probe in nucleic acids [0.03%]
氟核磁共振定量分析缓慢交换过程的新方法及其在核酸中2'-氟核糖探针的应用研究
Yuki Toyama,Ichio Shimada
Yuki Toyama
Solution NMR spectroscopy is a particularly powerful technique for characterizing the functional dynamics of biomolecules, which is typically achieved through the quantitative characterization of chemical exchange processes via the measurem...
Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR - practical aspects [0.03%]
高达140kDa的大分子可溶性蛋白质的沉降用于魔角旋转固体核磁共振和13C动态核极化固态核磁中的实际问题
Dallas Bell,Florian Lindemann,Lisa Gerland et al.
Dallas Bell et al.
Solution NMR is typically applied to biological systems with molecular weights < 40 kDa whereas magic-angle-spinning (MAS) solid-state NMR traditionally targets very large, oligomeric proteins and complexes exceeding 500 kDa in mass, includ...
Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers [0.03%]
利用配对的475和950MHz核磁共振光谱仪研究部分氘化的RNase H中的重氢自旋弛豫
Shibani Bhattacharya,Kristen M Varney,Tassadite Dahmane et al.
Shibani Bhattacharya et al.
Deuterium (2H) spin relaxation of 13CH2D methyl groups has been widely applied to investigate picosecond-to-nanosecond conformational dynamics in proteins by solution-state NMR spectroscopy. The B0 dependence of the 2H spin relaxation rates...
Labeling of methyl groups: a streamlined protocol and guidance for the selection of 2H precursors based on molecular weight [0.03%]
甲基同位素标记：一种精简的实验流程和基于分子量选择2H前体的指导原则
Alexandra Locke,Kylee Guarino,Gordon S Rule
Alexandra Locke
A streamlined one-day protocol is described to produce isotopically methyl-labeled protein with high levels of deuterium for NMR studies. Using this protocol, the D2O and 2H-glucose content of the media and protonation level of ILV labeling...
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression [0.03%]
利用毕赤酵母表达体系和5-氟色原酮前体对G蛋白偶联受体NK1R的色氨酸残基进行19F标记
Benxun Pan,Canyong Guo,Dongsheng Liu et al.
Benxun Pan et al.
In NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for...
Enabling site-specific NMR investigations of therapeutic Fab using a cell-free based isotopic labeling approach: application to anti-LAMP1 Fab [0.03%]
利用无细胞同位素标记方法对治疗性Fab的位点特异性NMR研究：抗LAMP1 Fab的应用
Arthur Giraud,Lionel Imbert,Adrien Favier et al.
Arthur Giraud et al.
Monoclonal antibodies (mAbs) are biotherapeutics that have achieved outstanding success in treating many life-threatening and chronic diseases. The recognition of an antigen is mediated by the fragment antigen binding (Fab) regions composed...
Prediction of order parameters based on protein NMR structure ensemble and machine learning [0.03%]
基于蛋白质NMR结构集合和机器学习的顺序参数预测
Qianqian Wang,Zhiwei Miao,Xiongjie Xiao et al.
Qianqian Wang et al.
The fast motions of proteins at the picosecond to nanosecond timescale, known as fast dynamics, are closely related to protein conformational entropy and rearrangement, which in turn affect catalysis, ligand binding and protein allosteric e...
DNP-assisted solid-state NMR enables detection of proteins at nanomolar concentrations in fully protonated cellular milieu [0.03%]
14ND-辅助固态NMR可在全质子细胞环境中检测纳摩尔浓度的蛋白质
Whitney N Costello,Yiling Xiao,Frederic Mentink-Vigier et al.
Whitney N Costello et al.
With the sensitivity enhancements conferred by dynamic nuclear polarization (DNP), magic angle spinning (MAS) solid state NMR spectroscopy experiments can attain the necessary sensitivity to detect very low concentrations of proteins. This ...
Decorating phenylalanine side-chains with triple labeled 13C/19F/2H isotope patterns [0.03%]
用13C/19F/2D三重同位素标记苯丙氨酸侧链装饰图案
Giorgia Toscano,Julian Holzinger,Benjamin Nagl et al.
Giorgia Toscano et al.
We present an economic and straightforward method to introduce 13C-19F spin systems into the deuterated aromatic side chains of phenylalanine as reporters for various protein NMR applications. The method is based on the synthesis of [4-13C,...