Labeling of methyl groups: a streamlined protocol and guidance for the selection of 2H precursors based on molecular weight
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A streamlined one-day protocol is described to produce isotopically methyl-labeled protein with high levels of deuterium for NMR studies. Using this protocol, the D2O and 2H-glucose content of the media and protonation level of ILV labeling precursors (ketobut... ...
