Spectral editing of intra- and inter-chain methyl-methyl NOEs in protein complexes [0.03%]
蛋白质复合物内和链间甲基-甲基NOE的光谱编辑
Ricarda Törner,Rida Awad,Pierre Gans et al.
Ricarda Törner et al.
Specific isotopic labeling of methyl groups in a perdeuterated protein background enables the detection of long range NOEs in proteins or high molecular weight complexes. We introduce here an approach, combining an optimized isotopic labeli...
Simultaneous recording of intra- and inter-residue linking experiments for backbone assignments in proteins at MAS frequencies higher than 60 kHz [0.03%]
在MAS频率高于60kHz时用于蛋白质主链共振 ASSIGNMENT的分子内和分子间连接实验的同时记录
Kshama Sharma,P K Madhu,Vipin Agarwal et al.
Kshama Sharma et al.
Obtaining site-specific assignments for the NMR spectra of proteins in the solid state is a significant bottleneck in deciphering their biophysics. This is primarily due to the time-intensive nature of the experiments. Additionally, the low...
Enhancing NMR derived ensembles with kinetics on multiple timescales [0.03%]
基于多重时间尺度动力学的核磁共振诱导集合增强法
Colin A Smith,Adam Mazur,Ashok K Rout et al.
Colin A Smith et al.
Nuclear magnetic resonance (NMR) has the unique advantage of elucidating the structure and dynamics of biomolecules in solution at physiological temperatures, where they are in constant movement on timescales from picoseconds to millisecond...
Non-uniform sampling in quantitative assessment of heterogeneous solid-state NMR line shapes [0.03%]
非均匀采样在异质固态NMR线宽定量评估中的应用
Ekaterina Burakova,Suresh K Vasa,Alexander Klein et al.
Ekaterina Burakova et al.
Non-uniform sampling has been successfully used for solution and solid-state NMR of homogeneous samples. In the solid state, protein samples are often dominated by inhomogeneous contributions to the homogeneous line widths. In spite of diff...
2-Fluorotyrosine is a valuable but understudied amino acid for protein-observed 19F NMR [0.03%]
氟代氨基酸2-氟酪氨酸在蛋白质观测型~(19)F NMR中的应用及前景
Peter D Ycas,Nicole Wagner,Noelle M Olsen et al.
Peter D Ycas et al.
Incorporation of 19F into proteins allows for the study of their molecular interactions via NMR. The study of 19F labeled aromatic amino acids has largely focused on 4-,5-, or 6-fluorotryptophan, 4-fluorophenylalanine, (4,5, or 6FW) or 3-fl...
Backbone and methyl assignment of bacteriorhodopsin incorporated into nanodiscs [0.03%]
结合纳米盘的视紫红质的主链和甲基归属
Laurens Kooijman,Philipp Ansorge,Matthias Schuster et al.
Laurens Kooijman et al.
Resonance assignments are challenging for membrane proteins due to the size of the lipid/detergent-protein complex and the presence of line-broadening from conformational exchange. As a consequence, many correlations are missing in the trip...
Daniel S Garrett,Mengli Cai,G Marius Clore
Daniel S Garrett
Here we present the XIPP (eXtensible Interactive Peak Picker) NMR software for analyzing multidimensional NMR data of proteins, DNA, RNA and protein-nucleic acid complexes. XIPP organizes experiments into pre-defined studies and replaces ou...
Intra-residue methyl-methyl correlations for valine and leucine residues in large proteins from a 3D-HMBC-HMQC experiment [0.03%]
利用三维HMBC-HMQC实验获得大蛋白质中缬氨酸和亮氨酸残基亚氨甲基的相关性
Lucas Siemons,Harold W Mackenzie,Vaibhav Kumar Shukla et al.
Lucas Siemons et al.
Methyl-TROSY based NMR experiments have over the last two decades become one of the most important means to characterise dynamics and functional mechanisms of large proteins and macromolecular machines in solution. The chemical shift assign...
A simple protocol for expression of isotope-labeled proteins in Escherichia coli grown in shaker flasks at high cell density [0.03%]
一种在摇瓶中高细胞密度下于大肠杆菌内表达同位素标记蛋白的简单方法
Mengli Cai,Ying Huang,Robert Craigie et al.
Mengli Cai et al.
Protein expression in E. coli grown in shaker flasks is a routine and pivotal tool in many research laboratories. To maximize protein yields, cells are normally induced in the middle of the linear growth phase, typically at an OD600 of ≤ 1...
Revisiting 1HN CPMG relaxation dispersion experiments: a simple modification can eliminate large artifacts [0.03%]
重新审视1HN CPMG弛豫分散实验：一个简单的修改可以消除大的伪像
Tairan Yuwen,Lewis E Kay
Tairan Yuwen
Carr-Purcell-Meiboom-Gill relaxation dispersion experiments are commonly used to probe biomolecular dynamics on the millisecond timescale. The simplest experiment involves using backbone 15N spins as probes of motion and pulse sequences are...