A simple protocol for expression of isotope-labeled proteins in Escherichia coli grown in shaker flasks at high cell density
{{output}}
Protein expression in E. coli grown in shaker flasks is a routine and pivotal tool in many research laboratories. To maximize protein yields, cells are normally induced in the middle of the linear growth phase, typically at an OD600 of ≤ 1 for cells grown in ... ...
