Development of in vitro-grown spheroids as a 3D tumor model system for solid-state NMR spectroscopy [0.03%]
体外培养类器官作为固体核磁共振光谱三维肿瘤模型系统的开发研究
Reinier Damman,Alessandra Lucini Paioni,Katerina T Xenaki et al.
Reinier Damman et al.
Recent advances in the field of in-cell NMR spectroscopy have made it possible to study proteins in the context of bacterial or mammalian cell extracts or even entire cells. As most mammalian cells are part of a multi-cellular complex, ther...
Efficient affinity ranking of fluorinated ligands by 19F NMR: CSAR and FastCSAR [0.03%]
高效氟谱磁共振亲和力排序：CSAR 和 FastCSAR
Simon H Rüdisser,Nils Goldberg,Marc-Olivier Ebert et al.
Simon H Rüdisser et al.
Fluorine NMR has recently gained high popularity in drug discovery as it allows efficient and sensitive screening of large numbers of ligands. However, the positive hits found in screening must subsequently be ranked according to their affi...
Sridhar Sreeramulu,Christian Richter,Till Kuehn et al.
Sridhar Sreeramulu et al.
Fragment-based screening has evolved as a remarkable approach within the drug discovery process both in the industry and academia. Fragment screening has become a more structure-based approach to inhibitor development, but also towards deve...
Charlotte A Softley,Mark J Bostock,Grzegorz M Popowicz et al.
Charlotte A Softley et al.
The presence of an unpaired electron in paramagnetic molecules generates significant effects in NMR spectra, which can be exploited to provide restraints complementary to those used in standard structure-calculation protocols. NMR already o...
Site-specific resolution of anionic residues in proteins using solid-state NMR spectroscopy [0.03%]
利用固态核磁共振技术对蛋白质中阴离子残基进行特异定位分辨研究
Jianping Li,Ampon Sae Her,Nathaniel J Traaseth
Jianping Li
NMR spectroscopy is commonly used to infer site-specific acid dissociation constants (pKa) since the chemical shift is sensitive to the protonation state. Methods that probe atoms nearest to the functional groups involved in acid/base chemi...
NMR mapping of the highly flexible regions of 13C/15N-labeled antibody TTAC-0001-Fab [0.03%]
用于TTAC-0001-Fab抗体的13C/15N标记NMR活性区域图谱绘制实验
Soyoung Cha,Weon Sup Lee,Joonhyeok Choi et al.
Soyoung Cha et al.
Monoclonal antibody (mAb) drugs are clinically important for the treatment of various diseases. TTAC-0001 is under development as a new anti-cancer antibody drug targeting VEGFR-2. As the less severe toxicity of TTAC-0001 compared to Bevaci...
Johnny Chen,Allan M Torres,Gang Zheng et al.
Johnny Chen et al.
WaterControl is a solvent suppression method based on WATERGATE and PGSTE and is very efficient in selectively reducing the solvent signal in 1D pulse-acquire and 2D NOESY of protein solutions. In this study, the WaterControl technique was ...
Backbone assignments and conformational dynamics in the S. typhimurium tryptophan synthase α-subunit from solution-state NMR [0.03%]
溶液态NMR研究的鼠伤寒沙门氏菌色氨酸合成酶α-亚基的骨干共振 assignment 及其结构动力学
Varun V Sakhrani,Eduardo Hilario,Bethany G Caulkins et al.
Varun V Sakhrani et al.
Backbone assignments for the isolated α-subunit of Salmonella typhimurium tryptophan synthase (TS) are reported based on triple resonance solution-state NMR experiments on a uniformly 2H,13C,15N-labeled sample. From the backbone chemical s...
Deleterious effects of carbon-carbon dipolar coupling on RNA NMR dynamics [0.03%]
碳-碳偶极耦合对RNA核磁共振动态学的负面影响
Hyeyeon Nam,Owen Becette,Regan M LeBlanc et al.
Hyeyeon Nam et al.
Many regulatory RNAs undergo dynamic exchanges that are crucial for their biological functions and NMR spectroscopy is a versatile tool for monitoring dynamic motions of biomolecules. Meaningful information on biomolecular dynamics requires...
Protein NMR of biologicals: analytical support for development and marketed products [0.03%]
生物药的蛋白质NMR分析：从研发到上市产品的支持
Fabio Casagrande,Klara Dégardin,Alfred Ross
Fabio Casagrande
Application of NMR spectroscopy to derive in-depth characterization of structure and dynamical properties of biomolecules is well established nowadays in many laboratories. Most of these methods rest on the availability of protein labeled w...