Discovery of Small-Molecule Inhibitors of the Pycsar Antiphage Defense [0.03%]
低分子量pycsar抗噬菌体防御抑制剂的发现
Marian A Addo,Vikram Damodaran,Joseph P Gerdt
Marian A Addo
The increasing prevalence of multidrug-resistant bacteria has intensified the search for alternative therapeutic strategies. Bacteriophages (the natural viral predators of bacteria) are one promising solution. However, bacterial immune syst...
Degrade to Display: Coupling Checkpoint Degradation with Exogenous Antigen Presentation to Boost Antitumor Immunity [0.03%]
降解以展示：结合检查点降解与外源性抗原呈递以增强抗肿瘤免疫响应
Yicong Ma,Jianfei Jiang,Yu Han
Yicong Ma
Membrane and extracellular targeted protein degradation (meTPD) has emerged as a revolutionary therapeutic modality against cancer, enabling the lysosomal clearance of traditionally undruggable oncogenic targets. However, the therapeutic po...
Targeting a Unique Cysteine Residue to Achieve Isoform-Selective Inhibition of the Proline Biosynthetic Enzyme Pyrroline-5-Carboxylate Reductase 2 [0.03%]
通过靶向独特半胱氨酸残基实现脯氨酸生物合成酶吡咯烷-5-羧酸还原酶2异构体选择性抑制
Tyrell C Rossman,Kaylen R Meeks,Gunjan Purohit et al.
Tyrell C Rossman et al.
Proline metabolism is selectively altered in cancer cells, providing ATP, redox balance, and proline for cell growth. The final enzyme of proline biosynthesis is Δ1-pyrroline-5-carboxylate (P5C) reductase (PYCR), which catalyzes the NAD(P)...
Natural Product-Derived Ianthelliformisamines Inhibit Protein Translation and Block Bacterial Flagellum Assembly [0.03%]
由天然产物衍生的Ianthelliformisamine抑制蛋白质翻译并阻止细菌鞭毛装配
Max Bottlinger,Martino Morici,Elena Fajardo-Ruiz et al.
Max Bottlinger et al.
Ianthelliformisamines (Ian) represent a poorly characterized natural product class reported to inhibit Gram-negative bacteria such as Escherichia coli. Given the current antibiotic crisis, revisiting poorly characterized antibacterial natur...
Genome Mining of Sphingopyxis sp. MG Uncovers an Enzyme with Dual Catalytic Activity of Haloalkane Dehalogenase and Epoxide Hydrolase [0.03%]
来源于Sphingopyxis sp. MG基因组挖掘的具有脱卤酶和内酯酶/环氧化物水解酶双催化活性的酶学研究
Annapoorni Lakshman Sagar,Ghufrana Abdus Sami,Wenhao Deng et al.
Annapoorni Lakshman Sagar et al.
Uncovering enzymes with promiscuous catalytic activity is significant in biocatalysis to expand their synthetic application toward sustainable chemical transformations, find non-native catalytic activity, and understand enzyme evolution. Mi...
Impact of Nucleotide Flexibility on Aptamer-Protein Recognition: RNA vs RNA-DNA Chimera [0.03%]
核苷酸柔韧性对适配体-蛋白质识别的影响：RNA与RNA-DNA嵌合体的比较研究
Takuya Hasegawa,Tomoki Sakamoto,Masahiro Sekiguchi et al.
Takuya Hasegawa et al.
Aptamers are single-stranded nucleic acid molecules that bind specifically and strongly to target molecules by adopting a unique tertiary structure that fits the target molecule. They have a wide range of biomedical applications, including ...
A High-Throughput Fluorescence Polarization Assay for Screening Sirtuin Inhibitors [0.03%]
一种高通量的荧光偏振筛选分屏SirT抑制剂的方法
Kewen Peng,Suryadeep Chakraborty,Yizhen Jin et al.
Kewen Peng et al.
Sirtuins (SIRTs), which remove protein lysine acyl modifications, play crucial roles in diverse cellular processes including metabolism, transcription, DNA damage repair, cell survival, and stress response. Several sirtuins are considered a...
Design, Synthesis, and Application of Sulfonium Diazo Probes for Red-Light Photocatalytic Proximity Labeling [0.03%]
用于红光催化邻近标记的锍叠氮探针的设计、合成与应用
Feifei Tong,Hong Kai Ng,Ciaran P Seath
Feifei Tong
Photocatalytic proximity labeling has become a versatile tool for probing biomolecular interactions, with growing interest in longer-wavelength activation, owing to its biocompatibility with various tissue types. Despite recent advances, hi...
Zhipeng Tao,Ritesh P Thakare,Melyssa Cheung et al.
Zhipeng Tao et al.
GSDMA, the primary member of the gasdermin family found in the skin, is critical for pathogen-induced pyroptosis during infection. Recent studies revealed that another gasdermin, GSDMD, undergoes palmitoylation during pyroptosis. However, w...
Expedient Chemical Synthesis of Ufmylated Histones Using Chemoenzymatic C-Terminal Hydrazinolysis of Ubiquitin-Fold Modifier 1 [0.03%]
利用泛素折叠修饰酶1的C端肼解的化学酶促合成UFMYlated组蛋白的新方法
Xin Liu,Cong Chen,Fang Li et al.
Xin Liu et al.
Ufmylation is a newly identified ubiquitin-like modification of histones and plays important roles in DNA-related processes. Dissecting histone ufmylation pathways necessitates the use of chemically defined proteins to assign their structur...