Gurushankar Chandramouly,Joonas Jamsen,Nikita Borisonnik et al.
Gurushankar Chandramouly et al.
The double-strand break (DSB) repair pathway called microhomology-mediated end-joining (MMEJ) is thought to be dependent on DNA polymerase theta (Polθ) and occur independently of nonhomologous end-joining (NHEJ) factors. An unresolved ques...
A pioneer factor locally opens compacted chromatin to enable targeted ATP-dependent nucleosome remodeling [0.03%]
一个先驱因子局部打开紧密的染色质以实现定向的ATP依赖型核小体重塑
Megan A Frederick,Kaylyn E Williamson,Meilin Fernandez Garcia et al.
Megan A Frederick et al.
To determine how different pioneer transcription factors form a targeted, accessible nucleosome within compacted chromatin and collaborate with an ATP-dependent chromatin remodeler, we generated nucleosome arrays in vitro with a central nuc...
A single 2'-O-methylation of ribosomal RNA gates assembly of a functional ribosome [0.03%]
核糖体RNA上的单一2'-O-甲基化控制功能核糖体的组装
James N Yelland,Jack P K Bravo,Joshua J Black et al.
James N Yelland et al.
RNA modifications are widespread in biology and abundant in ribosomal RNA. However, the importance of these modifications is not well understood. We show that methylation of a single nucleotide, in the catalytic center of the large subunit,...
Sherry Bermeo,Andrew Favor,Ya-Ting Chang et al.
Sherry Bermeo et al.
The de novo design of three protein chains that associate to form a heterotrimer (but not any of the possible two-chain heterodimers) and that can drive the assembly of higher-order branching structures is an important challenge for protein...
Zachary Lee Johnson,Mark Ammirati,David Jonathan Wasilko et al.
Zachary Lee Johnson et al.
Glycerol-3-phosphate acyltransferase (GPAT)1 is a mitochondrial outer membrane protein that catalyzes the first step of de novo glycerolipid biosynthesis. Hepatic expression of GPAT1 is linked to liver fat accumulation and the severity of n...
Sonja Rutz,Dawid Deneka,Antje Dittmann et al.
Sonja Rutz et al.
Volume-regulated anion channels (VRACs) participate in the cellular response to osmotic swelling. These membrane proteins consist of heteromeric assemblies of LRRC8 subunits, whose compositions determine permeation properties. Although stru...
Cooperation of chromatin remodeling SWI/SNF complex and pioneer factor AP-1 shapes 3D enhancer landscapes [0.03%]
染色质重塑SWI / SNF复合物与先驱因子AP-1的配合塑造三维增强子景观
Bennett K Wolf,Yanding Zhao,Andrew McCray et al.
Bennett K Wolf et al.
The mechanism controlling the dynamic targeting of SWI/SNF has long been postulated to be coordinated by transcription factors (TFs), yet demonstrating a specific TF influence has proven difficult. Here we take a multi-omics approach to int...
Structural basis for Parkinson's disease-linked LRRK2's binding to microtubules [0.03%]
帕金森氏症相关LRRK2结合微管的结构基础
David M Snead,Mariusz Matyszewski,Andrea M Dickey et al.
David M Snead et al.
Leucine-rich repeat kinase 2 (LRRK2) is one of the most commonly mutated genes in familial Parkinson's disease (PD). Under some circumstances, LRRK2 co-localizes with microtubules in cells, an association enhanced by PD mutations. We report...
DNA methyltransferases 3A and 3B target specific sequences during mouse gastrulation [0.03%]
DNA甲基转移酶3A和3B在小鼠原肠形成过程中靶向特定序列
Zohar Mukamel,Aviezer Lifshitz,Markus Mittnenzweig et al.
Zohar Mukamel et al.
In mammalian embryos, DNA methylation is initialized to maximum levels in the epiblast by the de novo DNA methyltransferases DNMT3A and DNMT3B before gastrulation diversifies it across regulatory regions. Here we show that DNMT3A and DNMT3B...
Judith Barbara Zaugg,Pelin Sahlén,Robin Andersson et al.
Judith Barbara Zaugg et al.
Enhancers play a central role in the spatiotemporal control of gene expression and tend to work in a cell-type-specific manner. In addition, they are suggested to be major contributors to phenotypic variation, evolution and disease. There i...