Katrina V Good,Juan Ausió
Katrina V Good
A tweak and a peek: How Cas9 pries open double-stranded DNA to check its sequence [0.03%]
一探与二看：Cas9 如何撬开双链DNA以检查其序列
Selma Sinan,Rick Russell
Selma Sinan
Joshua C Cofsky,Katarzyna M Soczek,Gavin J Knott et al.
Joshua C Cofsky et al.
In bacterial defense and genome editing applications, the CRISPR-associated protein Cas9 searches millions of DNA base pairs to locate a 20-nucleotide, guide RNA-complementary target sequence that abuts a protospacer-adjacent motif (PAM). T...
David Jeruzalmi
David Jeruzalmi
Beth Moorefield
Beth Moorefield
Jie Li,Junhee Park,John P Mayer et al.
Jie Li et al.
Insulin receptor (IR) signaling controls multiple facets of animal physiology. Maximally four insulins bind to IR at two distinct sites, termed site-1 and site-2. However, the precise functional roles of each binding event during IR activat...
Structural basis of an endocytic checkpoint that primes the AP2 clathrin adaptor for cargo internalization [0.03%]
AP2适配体-clathrin核小体结构调控内吞的分子机制研究
Edward A Partlow,Kevin S Cannon,Gunther Hollopeter et al.
Edward A Partlow et al.
Clathrin-mediated endocytosis (CME) is the main route of internalization from the plasma membrane. It is known that the heterotetrameric AP2 clathrin adaptor must open to simultaneously engage membrane and endocytic cargo, yet it is unclear...
Short- and long-range interactions in the HIV-1 5' UTR regulate genome dimerization and packaging [0.03%]
HIV-1 5'-UTR的长程和短程相互作用调控基因组二聚化与包装
Liqing Ye,Anne-Sophie Gribling-Burrer,Patrick Bohn et al.
Liqing Ye et al.
RNA dimerization is the noncovalent association of two human immunodeficiency virus-1 (HIV-1) genomes. It is a conserved step in the HIV-1 life cycle and assumed to be a prerequisite for binding to the viral structural protein Pr55Gag durin...
Structure of the human GlcNAc-1-phosphotransferase αβ subunits reveals regulatory mechanism for lysosomal enzyme glycan phosphorylation [0.03%]
人GlcNAc-1-磷酸转移酶αβ亚基结构揭示了溶酶体酶糖基磷酸化的调控机制
Hua Li,Wang-Sik Lee,Xiang Feng et al.
Hua Li et al.
Vertebrates use the mannose 6-phosphate (M6P)-recognition system to deliver lysosomal hydrolases to lysosomes. Key to this pathway is N-acetylglucosamine (GlcNAc)-1-phosphotransferase (PTase) that selectively adds GlcNAc-phosphate (P) to ma...
Bound nucleotide can control the dynamic architecture of monomeric actin [0.03%]
结合核苷酸可以控制单体肌动蛋白的动态结构
Rustam Ali,Jacob A Zahm,Michael K Rosen
Rustam Ali
Polymerization of actin into cytoskeletal filaments is coupled to its bound adenine nucleotides. The mechanism by which nucleotide modulates actin functions has not been evident from analyses of ATP- and ADP-bound crystal structures of the ...