Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase [0.03%]
DNA装载的MCM双六聚体选择性地被Dbf4依赖性激酶磷酸化的结构机制
Julia F Greiwe,Thomas C R Miller,Julia Locke et al.
Julia F Greiwe et al.
Loading of the eukaryotic replicative helicase onto replication origins involves two MCM hexamers forming a double hexamer (DH) around duplex DNA. During S phase, helicase activation requires MCM phosphorylation by Dbf4-dependent kinase (DD...
ESKAPE velocity: total synthesis platforms promise to increase the pace and diversity of antibiotic development [0.03%]
ESKAPE速度：-total合成平台有望加快和增加抗生素开发的速度和多样性
Jacob M Mattingly,Christine M Dunham
Jacob M Mattingly
Decoupling of degradation from deadenylation reshapes poly(A) tail length in yeast meiosis [0.03%]
酵母减数分裂中降解与去腺苷化的解耦重塑多（A）尾长度
David Wiener,Yaron Antebi,Schraga Schwartz
David Wiener
Nascent messenger RNA is endowed with a poly(A) tail that is subject to gradual deadenylation and subsequent degradation in the cytoplasm. Deadenylation and degradation rates are typically correlated, rendering it difficult to dissect the d...
Fungicidal amphotericin B sponges are assemblies of staggered asymmetric homodimers encasing large void volumes [0.03%]
fungicidal两性霉素B海绵是阶梯式不对称同二聚体的集合，包含大量空腔体积
Agnieszka Lewandowska,Corinne P Soutar,Alexander I Greenwood et al.
Agnieszka Lewandowska et al.
Amphotericin B (AmB) is a powerful but toxic fungicide that operates via enigmatic small molecule-small molecule interactions. This mechanism has challenged the frontiers of structural biology for half a century. We recently showed AmB prim...
Agata D Misiaszek,Mathias Girbig,Helga Grötsch et al.
Agata D Misiaszek et al.
RNA polymerase I (Pol I) specifically synthesizes ribosomal RNA. Pol I upregulation is linked to cancer, while mutations in the Pol I machinery lead to developmental disorders. Here we report the cryo-EM structure of elongating human Pol I ...
Carolina N Perdigoto
Carolina N Perdigoto
Florian Ullrich
Florian Ullrich
Sara Osman
Sara Osman
Alexander F A Keszei,Matthew C J Yip,Ta-Chien Hsieh et al.
Alexander F A Keszei et al.
Close coordination between chaperones is essential for protein biosynthesis, including the delivery of tail-anchored (TA) proteins containing a single C-terminal transmembrane domain to the endoplasmic reticulum (ER) by the conserved GET pa...
Evolution of a histone variant involved in compartmental regulation of NAD metabolism [0.03%]
参与NAD代谢隔室调节的组蛋白变体的进化
Iva Guberovic,Sarah Hurtado-Bagès,Ciro Rivera-Casas et al.
Iva Guberovic et al.
NAD metabolism is essential for all forms of life. Compartmental regulation of NAD+ consumption, especially between the nucleus and the mitochondria, is required for energy homeostasis. However, how compartmental regulation evolved remains ...