The cohesin acetylation cycle controls chromatin loop length through a PDS5A brake mechanism [0.03%]
乙酰化循环通过PDS5A制动机制控制染色质环的长度
Marjon S van Ruiten,Démi van Gent,Ángela Sedeño Cacciatore et al.
Marjon S van Ruiten et al.
Cohesin structures the genome through the formation of chromatin loops and by holding together the sister chromatids. The acetylation of cohesin's SMC3 subunit is a dynamic process that involves the acetyltransferase ESCO1 and deacetylase H...
Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops [0.03%]
SMC3乙酰化、PDS5和SCC2控制建立凝聚素依赖性染色质环的转位酶活性
Nathalie Bastié,Christophe Chapard,Lise Dauban et al.
Nathalie Bastié et al.
Cohesin is a DNA translocase that is instrumental in the folding of the genome into chromatin loops, with functional consequences on DNA-related processes. Chromatin loop length and organization likely depend on cohesin processivity, transl...
Screening thousands of transcribed coding and non-coding regions reveals sequence determinants of RNA polymerase II elongation potential [0.03%]
筛选转录的编码和非编码区揭示RNA聚合酶II延长位点的序列决定因素
Hanneke Vlaming,Claudia A Mimoso,Andrew R Field et al.
Hanneke Vlaming et al.
Precise regulation of transcription by RNA polymerase II (RNAPII) is critical for organismal growth and development. However, what determines whether an engaged RNAPII will synthesize a full-length transcript or terminate prematurely is poo...
Structure, dynamics and assembly of the ankyrin complex on human red blood cell membrane [0.03%]
人类红细胞膜上锚蛋白复合物的结构、动力学和组装机制研究
Xian Xia,Shiheng Liu,Z Hong Zhou
Xian Xia
The cytoskeleton of a red blood cell (RBC) is anchored to the cell membrane by the ankyrin complex. This complex is assembled during RBC genesis and comprises primarily band 3, protein 4.2 and ankyrin, whose mutations contribute to numerous...
Quaternary structure independent folding of voltage-gated ion channel pore domain subunits [0.03%]
电压门控离子通道孔道结构域亚单位的四级结构独立折叠现象
Cristina Arrigoni,Marco Lolicato,David Shaya et al.
Cristina Arrigoni et al.
Every voltage-gated ion channel (VGIC) has a pore domain (PD) made from four subunits, each comprising an antiparallel transmembrane helix pair bridged by a loop. The extent to which PD subunit structure requires quaternary interactions is ...
Structural insights into binding of therapeutic channel blockers in NMDA receptors [0.03%]
治疗性通道阻滞剂与N-甲基-D天冬氨酸受体结合的结构洞察
Tsung-Han Chou,Max Epstein,Kevin Michalski et al.
Tsung-Han Chou et al.
Excitatory signaling mediated by N-methyl-D-aspartate receptor (NMDAR) is critical for brain development and function, as well as for neurological diseases and disorders. Channel blockers of NMDARs are of medical interest owing to their pot...
Identifying amyloid-related diseases by mapping mutations in low-complexity protein domains to pathologies [0.03%]
通过在低复杂性蛋白质结构域中绘制突变来识别淀粉样蛋白相关疾病
Kevin A Murray,Michael P Hughes,Carolyn J Hu et al.
Kevin A Murray et al.
Proteins including FUS, hnRNPA2, and TDP-43 reversibly aggregate into amyloid-like fibrils through interactions of their low-complexity domains (LCDs). Mutations in LCDs can promote irreversible amyloid aggregation and disease. We introduce...
Mammalian PERIOD2 regulates H2A.Z incorporation in chromatin to orchestrate circadian negative feedback [0.03%]
哺乳动物PERIOD2通过调节组蛋白H2A.Z在染色质中的镶嵌来调控昼夜节律负反馈循环
Kevin Tartour,Francesca Andriani,Eric G Folco et al.
Kevin Tartour et al.
Mammalian circadian oscillators are built on a feedback loop in which the activity of the transcription factor CLOCK-BMAL1 is repressed by the PER-CRY complex. Here, we show that murine Per-/- fibroblasts display aberrant nucleosome occupan...
Allosteric regulation controls actin-bundling properties of human plastins [0.03%]
别构调节控制人类plastin的肌动蛋白聚集性质
Christopher L Schwebach,Elena Kudryashova,Richa Agrawal et al.
Christopher L Schwebach et al.
Plastins/fimbrins are conserved actin-bundling proteins contributing to motility, cytokinesis and other cellular processes by organizing strikingly different actin assemblies as in aligned bundles and branched networks. We propose that this...
Histone H1 binding to nucleosome arrays depends on linker DNA length and trajectory [0.03%]
组蛋白H1与核小体阵列的结合依赖于链接DNA的长度和轨迹
Marco Dombrowski,Maik Engeholm,Christian Dienemann et al.
Marco Dombrowski et al.
Throughout the genome, nucleosomes often form regular arrays that differ in nucleosome repeat length (NRL), occupancy of linker histone H1 and transcriptional activity. Here, we report cryo-EM structures of human H1-containing tetranucleoso...