Structural basis of CHMP2A-CHMP3 ESCRT-III polymer assembly and membrane cleavage [0.03%]
CHMP2A-CHMP3 ESCRT-III聚合组装和膜切割的结构基础
Kimi Azad,Delphine Guilligay,Cecile Boscheron et al.
Kimi Azad et al.
The endosomal sorting complex required for transport (ESCRT) is a highly conserved protein machinery that drives a divers set of physiological and pathological membrane remodeling processes. However, the structural basis of ESCRT-III polyme...
The molecular structure of IFT-A and IFT-B in anterograde intraflagellar transport trains [0.03%]
前向纤毛内运输列车中IFT-A和IFT-B的分子结构
Samuel E Lacey,Helen E Foster,Gaia Pigino
Samuel E Lacey
Anterograde intraflagellar transport (IFT) trains are essential for cilia assembly and maintenance. These trains are formed of 22 IFT-A and IFT-B proteins that link structural and signaling cargos to microtubule motors for import into cilia...
Replication fork uncoupling causes nascent strand degradation and fork reversal [0.03%]
复制叉解离导致新生链降解和叉逆转
Tamar Kavlashvili,Wenpeng Liu,Taha M Mohamed et al.
Tamar Kavlashvili et al.
Genotoxins cause nascent strand degradation (NSD) and fork reversal during DNA replication. NSD and fork reversal are crucial for genome stability and are exploited by chemotherapeutic approaches. However, it is unclear how NSD and fork rev...
Quinton M Dowling,Hannah E Volkman,Elizabeth E Gray et al.
Quinton M Dowling et al.
Cyclic GMP-AMP synthase (cGAS) is a pattern recognition receptor critical for the innate immune response to intracellular pathogens, DNA damage, tumorigenesis and senescence. Binding to double-stranded DNA (dsDNA) induces conformational cha...
Genetic code expansion reveals aminoacylated lysine ubiquitination mediated by UBE2W [0.03%]
遗传密码扩展揭示了由UBE2W介导的氨基酰化赖氨酸泛素化过程
Jia Zang,Yulin Chen,Chao Liu et al.
Jia Zang et al.
Protein post-translational modification (PTM) regulates nearly every aspect of cellular processes in eukaryotes. However, the identification of new protein PTMs is very challenging. Here, using genetically encoded unnatural amino acids as c...
In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes [0.03%]
原位冷冻电子断层成像揭示了哺乳动物精子轴丝的非对称结构
Zhen Chen,Garrett A Greenan,Momoko Shiozaki et al.
Zhen Chen et al.
The flagella of mammalian sperm display non-planar, asymmetric beating, in contrast to the planar, symmetric beating of flagella from sea urchin sperm and unicellular organisms. The molecular basis of this difference is unclear. Here, we pe...
Michael R Lieber
Michael R Lieber
New work on DNA polymerase λ highlights its remarkable flexibility. This fits with the generally adaptable nature of the DNA-repair process in which this enzyme is involved — nonhomologous end-joining — which allows this mechanism to han...
Actin up: shifting chromosomes toward repair, but also translocations [0.03%]
肌动蛋白的活跃作用：染色体向修复位移，同时也可能发生易位
Heng Li,Rachel Patton McCord
Heng Li
Dimitris Typas
Dimitris Typas
Multiscale reorganization of the genome following DNA damage facilitates chromosome translocations via nuclear actin polymerization [0.03%]
DNA损伤后通过核肌动蛋白聚合促进染色体易位的多尺度基因组重构
Jennifer Zagelbaum,Allana Schooley,Junfei Zhao et al.
Jennifer Zagelbaum et al.
Nuclear actin-based movements have been shown to orchestrate clustering of DNA double-strand breaks (DSBs) into homology-directed repair domains. Here we describe multiscale three-dimensional genome reorganization following DNA damage and a...