Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1 [0.03%]
可视化由AAA-ATP酶Drg1从新生核糖体提取成熟因子的过程
Michael Prattes,Irina Grishkovskaya,Victor-Valentin Hodirnau et al.
Michael Prattes et al.
The AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from pre-60S particles shortly after nuclear exp...
Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancer [0.03%]
低聚糖负荷的mRNA转录本解析确定了肾癌中缺氧诱导因子和哺乳动物雷帕霉素靶点的失调交互作用
Yoichiro Sugimoto,Peter J Ratcliffe
Yoichiro Sugimoto
Hypoxia inducible factor (HIF) and mammalian target of rapamycin (mTOR) pathways orchestrate responses to oxygen and nutrient availability. These pathways are frequently dysregulated in cancer, but their interplay is poorly understood, in p...
Cohesin is required for long-range enhancer action at the Shh locus [0.03%]
凝缩素蛋白对于Shh基因座长程增强子作用是必需的
Lauren Kane,Iain Williamson,Ilya M Flyamer et al.
Lauren Kane et al.
The regulatory landscapes of developmental genes in mammals can be complex, with enhancers spread over many hundreds of kilobases. It has been suggested that three-dimensional genome organization, particularly topologically associating doma...
Cryo-EM structure of disease-related prion fibrils provides insights into seeding barriers [0.03%]
与疾病相关的朊病毒原纤维的冷冻电镜结构提供了关于播种障碍的见解
Qiuye Li,Christopher P Jaroniec,Witold K Surewicz
Qiuye Li
One of the least understood aspects of prion diseases is the structure of infectious prion protein aggregates. Here we report a high-resolution cryo-EM structure of amyloid fibrils formed by human prion protein with the Y145Stop mutation th...
High-resolution structures of the SARS-CoV-2 N7-methyltransferase inform therapeutic development [0.03%]
SARS-CoV-2 N7-甲基转移酶的高分辨率结构为药物研发提供信息
Jithesh Kottur,Olga Rechkoblit,Richard Quintana-Feliciano et al.
Jithesh Kottur et al.
Emergence of SARS-CoV-2 coronavirus has led to millions of deaths globally. We present three high-resolution crystal structures of the SARS-CoV-2 nsp14 N7-methyltransferase core bound to S-adenosylmethionine (1.62 Å), S-adenosylhomocystein...
Cody M Rogers,Patrick Sung
Cody M Rogers
Histone deacetylation primes self-propagation of heterochromatin domains to promote epigenetic inheritance [0.03%]
组蛋白去乙酰化促进异染色质域的自我传播以促进表观遗传学继承
Martin Zofall,Rima Sandhu,Sahana Holla et al.
Martin Zofall et al.
Heterochromatin assembly, involving histone H3 lysine-9 methylation (H3K9me), is nucleated at specific genomic sites but can self-propagate across extended domains and, indeed, generations. Self-propagation requires Clr4/Suv39h methyltransf...
The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination [0.03%]
DNA损伤激酶ATR通过泛素化起始激活FANCD2-FANCI滑动夹复合体
Tamara Sijacki,Pablo Alcón,Zhuo A Chen et al.
Tamara Sijacki et al.
DNA interstrand cross-links are tumor-inducing lesions that block DNA replication and transcription. When cross-links are detected at stalled replication forks, ATR kinase phosphorylates FANCI, which stimulates monoubiquitination of the FAN...
Structure of CRL7FBXW8 reveals coupling with CUL1-RBX1/ROC1 for multi-cullin-RING E3-catalyzed ubiquitin ligation [0.03%]
CRL7FBXW8 结构揭示了与 CUL1-RBX1/ROC1 耦合以催化多 cullin-RING E3 泛素连接酶的机制
Linus V M Hopf,Kheewoong Baek,Maren Klügel et al.
Linus V M Hopf et al.
Most cullin-RING ubiquitin ligases (CRLs) form homologous assemblies between a neddylated cullin-RING catalytic module and a variable substrate-binding receptor (for example, an F-box protein). However, the vertebrate-specific CRL7FBXW8 is ...
Structural identification of lysophosphatidylcholines as activating ligands for orphan receptor GPR119 [0.03%]
孤儿受体GPR119的活化配体溶血磷脂酰胆碱的结构鉴定
Peiyu Xu,Sijie Huang,Shimeng Guo et al.
Peiyu Xu et al.
Lysophosphatidylcholine (LPC) is an essential mediator in human lipid metabolism and is associated with a variety of diseases, but the exact identity of LPC receptors remains controversial. Through extensive biochemical and structural analy...