Joseph Willson
Joseph Willson
Sequence-specific remodeling of a topologically complex RNP substrate by Spb4 [0.03%]
Spb4对拓扑复杂RNP底物的特异性重塑
Victor Emmanuel Cruz,Kamil Sekulski,Nagesh Peddada et al.
Victor Emmanuel Cruz et al.
DEAD-box ATPases are ubiquitous enzymes essential in all aspects of RNA biology. However, the limited in vitro catalytic activities described for these enzymes are at odds with their complex cellular roles, most notably in driving large-sca...
Porter M Hall,James T Inman,Robert M Fulbright et al.
Porter M Hall et al.
CRISPR (clustered regularly interspaced short palindromic repeats) utility relies on a stable Cas effector complex binding to its target site. However, a Cas complex bound to DNA may be removed by motor proteins carrying out host processes ...
Aakash Basu,Dmitriy G Bobrovnikov,Basilio Cieza et al.
Aakash Basu et al.
Diverse DNA-deforming processes are impacted by the local mechanical and structural properties of DNA, which in turn depend on local sequence and epigenetic modifications. Deciphering this mechanical code (that is, this dependence) has been...
Babatunde Ekundayo,Davide Torre,Bertrand Beckert et al.
Babatunde Ekundayo et al.
The CRISPR-guided caspase (Craspase) complex is an assembly of the target-specific RNA nuclease known as Cas7-11 bound to CRISPR RNA (crRNA) and an ancillary protein known as TPR-CHAT (tetratricopeptide repeats (TPR) fused with a CHAT domai...
Mechanism of cyclic β-glucan export by ABC transporter Cgt of Brucella [0.03%]
布鲁氏菌ABC转运蛋白Cgt输出环状β-葡聚糖的机制
Jaroslaw Sedzicki,Dongchun Ni,Frank Lehmann et al.
Jaroslaw Sedzicki et al.
Polysaccharides play critical roles in bacteria, including the formation of protective capsules and biofilms and establishing specific host cell interactions. Their transport across membranes is often mediated by ATP-binding cassette (ABC) ...
Jiangtao Zhang,Yiqiang Shi,Zhuo Huang et al.
Jiangtao Zhang et al.
Voltage-gated sodium channel NaV1.7 plays essential roles in pain and odor perception. NaV1.7 variants cause pain disorders. Accordingly, NaV1.7 has elicited extensive attention in developing new analgesics. Here we present cryo-EM structur...
Structural basis of SNAPc-dependent snRNA transcription initiation by RNA polymerase II [0.03%]
RNA聚合酶II依赖SNAPc进行snRNA转录起始的结构基础
Srinivasan Rengachari,Sandra Schilbach,Thangavelu Kaliyappan et al.
Srinivasan Rengachari et al.
RNA polymerase II (Pol II) carries out transcription of both protein-coding and non-coding genes. Whereas Pol II initiation at protein-coding genes has been studied in detail, Pol II initiation at non-coding genes, such as small nuclear RNA...
Structures of transcription preinitiation complex engaged with the +1 nucleosome [0.03%]
与+1核小体结合的转录前起始复合物的结构
Haibo Wang,Sandra Schilbach,Momchil Ninov et al.
Haibo Wang et al.
The preinitiation complex (PIC) assembles on promoters of protein-coding genes to position RNA polymerase II (Pol II) for transcription initiation. Previous structural studies revealed the PIC on different promoters, but did not address how...
Structure determination of inactive-state GPCRs with a universal nanobody [0.03%]
通用纳米体介导的无活性状态GPCR结构测定
Michael J Robertson,Makaía M Papasergi-Scott,Feng He et al.
Michael J Robertson et al.
Cryogenic electron microscopy (cryo-EM) has widened the field of structure-based drug discovery by allowing for routine determination of membrane protein structures previously intractable. Despite representing one of the largest classes of ...