In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes [0.03%]
原位冷冻电子断层成像揭示了哺乳动物精子轴丝的非对称结构
Zhen Chen,Garrett A Greenan,Momoko Shiozaki et al.
Zhen Chen et al.
The flagella of mammalian sperm display non-planar, asymmetric beating, in contrast to the planar, symmetric beating of flagella from sea urchin sperm and unicellular organisms. The molecular basis of this difference is unclear. Here, we pe...
Michael R Lieber
Michael R Lieber
New work on DNA polymerase λ highlights its remarkable flexibility. This fits with the generally adaptable nature of the DNA-repair process in which this enzyme is involved — nonhomologous end-joining — which allows this mechanism to han...
Actin up: shifting chromosomes toward repair, but also translocations [0.03%]
肌动蛋白的活跃作用：染色体向修复位移，同时也可能发生易位
Heng Li,Rachel Patton McCord
Heng Li
Dimitris Typas
Dimitris Typas
Multiscale reorganization of the genome following DNA damage facilitates chromosome translocations via nuclear actin polymerization [0.03%]
DNA损伤后通过核肌动蛋白聚合促进染色体易位的多尺度基因组重构
Jennifer Zagelbaum,Allana Schooley,Junfei Zhao et al.
Jennifer Zagelbaum et al.
Nuclear actin-based movements have been shown to orchestrate clustering of DNA double-strand breaks (DSBs) into homology-directed repair domains. Here we describe multiscale three-dimensional genome reorganization following DNA damage and a...
Diverse silent chromatin states modulate genome compartmentalization and loop extrusion barriers [0.03%]
多种沉默染色质状态调节基因组隔室化和环域排除障碍
George Spracklin,Nezar Abdennur,Maxim Imakaev et al.
George Spracklin et al.
The relationships between chromosomal compartmentalization, chromatin state and function are poorly understood. Here by profiling long-range contact frequencies in HCT116 colon cancer cells, we distinguish three silent chromatin states, com...
Gurushankar Chandramouly,Joonas Jamsen,Nikita Borisonnik et al.
Gurushankar Chandramouly et al.
The double-strand break (DSB) repair pathway called microhomology-mediated end-joining (MMEJ) is thought to be dependent on DNA polymerase theta (Polθ) and occur independently of nonhomologous end-joining (NHEJ) factors. An unresolved ques...
A pioneer factor locally opens compacted chromatin to enable targeted ATP-dependent nucleosome remodeling [0.03%]
一个先驱因子局部打开紧密的染色质以实现定向的ATP依赖型核小体重塑
Megan A Frederick,Kaylyn E Williamson,Meilin Fernandez Garcia et al.
Megan A Frederick et al.
To determine how different pioneer transcription factors form a targeted, accessible nucleosome within compacted chromatin and collaborate with an ATP-dependent chromatin remodeler, we generated nucleosome arrays in vitro with a central nuc...
A single 2'-O-methylation of ribosomal RNA gates assembly of a functional ribosome [0.03%]
核糖体RNA上的单一2'-O-甲基化控制功能核糖体的组装
James N Yelland,Jack P K Bravo,Joshua J Black et al.
James N Yelland et al.
RNA modifications are widespread in biology and abundant in ribosomal RNA. However, the importance of these modifications is not well understood. We show that methylation of a single nucleotide, in the catalytic center of the large subunit,...
Sherry Bermeo,Andrew Favor,Ya-Ting Chang et al.
Sherry Bermeo et al.
The de novo design of three protein chains that associate to form a heterotrimer (but not any of the possible two-chain heterodimers) and that can drive the assembly of higher-order branching structures is an important challenge for protein...