Brominated lipid probes expose structural asymmetries in constricted membranes [0.03%]
溴化的脂质探针揭示了受限制膜结构中的不对称性
Frank R Moss rd,James Lincoff,Maxwell Tucker et al.
Frank R Moss rd et al.
Lipids in biological membranes are thought to be functionally organized, but few experimental tools can probe nanoscale membrane structure. Using brominated lipids as contrast probes for cryo-EM and a model ESCRT-III membrane-remodeling sys...
HIV-1 is dependent on its immature lattice to recruit IP6 for mature capsid assembly [0.03%]
HIV-1依赖其未成熟的格子来募集IP6以完成成熟的核心组装
Nadine Renner,Alex Kleinpeter,Donna L Mallery et al.
Nadine Renner et al.
HIV-1 Gag metamorphoses inside each virion, from an immature lattice that forms during viral production to a mature capsid that drives infection. Here we show that the immature lattice is required to concentrate the cellular metabolite inos...
Hironori Takeda,Jon V Busto,Caroline Lindau et al.
Hironori Takeda et al.
Mitochondrial β-barrel proteins are essential for the transport of metabolites, ions and proteins. The sorting and assembly machinery (SAM) mediates their folding and membrane insertion. We report the cryo-electron microscopy structure of ...
Mechanism of NAIP-NLRC4 inflammasome activation revealed by cryo-EM structure of unliganded NAIP5 [0.03%]
冷冻电镜解析无配基NAIP5结构揭示了NAIP-NLRC4炎性小体激活的机制
Bhaskar Paidimuddala,Jianhao Cao,Grady Nash et al.
Bhaskar Paidimuddala et al.
The nucleotide-binding domain (NBD), leucine rich repeat (LRR) domain containing protein family (NLR family) apoptosis inhibitory proteins (NAIPs) are cytosolic receptors that play critical roles in the host defense against bacterial infect...
Shikang Liang,Tom L Blundell
Shikang Liang
DNA-dependent protein kinase (DNA-PK), a multicomponent complex including the DNA-PK catalytic subunit and Ku70/80 heterodimer together with DNA, is central to human DNA damage response and repair. Using a DNA-PK-selective inhibitor (M3814)...
Structural basis of CHMP2A-CHMP3 ESCRT-III polymer assembly and membrane cleavage [0.03%]
CHMP2A-CHMP3 ESCRT-III聚合组装和膜切割的结构基础
Kimi Azad,Delphine Guilligay,Cecile Boscheron et al.
Kimi Azad et al.
The endosomal sorting complex required for transport (ESCRT) is a highly conserved protein machinery that drives a divers set of physiological and pathological membrane remodeling processes. However, the structural basis of ESCRT-III polyme...
The molecular structure of IFT-A and IFT-B in anterograde intraflagellar transport trains [0.03%]
前向纤毛内运输列车中IFT-A和IFT-B的分子结构
Samuel E Lacey,Helen E Foster,Gaia Pigino
Samuel E Lacey
Anterograde intraflagellar transport (IFT) trains are essential for cilia assembly and maintenance. These trains are formed of 22 IFT-A and IFT-B proteins that link structural and signaling cargos to microtubule motors for import into cilia...
Replication fork uncoupling causes nascent strand degradation and fork reversal [0.03%]
复制叉解离导致新生链降解和叉逆转
Tamar Kavlashvili,Wenpeng Liu,Taha M Mohamed et al.
Tamar Kavlashvili et al.
Genotoxins cause nascent strand degradation (NSD) and fork reversal during DNA replication. NSD and fork reversal are crucial for genome stability and are exploited by chemotherapeutic approaches. However, it is unclear how NSD and fork rev...
Quinton M Dowling,Hannah E Volkman,Elizabeth E Gray et al.
Quinton M Dowling et al.
Cyclic GMP-AMP synthase (cGAS) is a pattern recognition receptor critical for the innate immune response to intracellular pathogens, DNA damage, tumorigenesis and senescence. Binding to double-stranded DNA (dsDNA) induces conformational cha...
Genetic code expansion reveals aminoacylated lysine ubiquitination mediated by UBE2W [0.03%]
遗传密码扩展揭示了由UBE2W介导的氨基酰化赖氨酸泛素化过程
Jia Zang,Yulin Chen,Chao Liu et al.
Jia Zang et al.
Protein post-translational modification (PTM) regulates nearly every aspect of cellular processes in eukaryotes. However, the identification of new protein PTMs is very challenging. Here, using genetically encoded unnatural amino acids as c...