Dynamic epistasis analysis reveals how chromatin remodeling regulates transcriptional bursting [0.03%]
动态上位基因效应分析揭示染色质重塑如何调节转录爆发
Ineke Brouwer,Emma Kerklingh,Fred van Leeuwen et al.
Ineke Brouwer et al.
Transcriptional bursting has been linked to the stochastic positioning of nucleosomes. However, how bursting is regulated by the remodeling of promoter nucleosomes is unknown. Here, we use single-molecule live-cell imaging of GAL10 transcri...
Yao Xiao,Shintaro Maeda,Takanori Otomo et al.
Yao Xiao et al.
Argonaute (AGO) proteins use small RNAs to recognize transcripts targeted for silencing in plants and animals. Many AGOs cleave target RNAs using an endoribonuclease activity termed 'slicing'. Slicing by DNA-guided prokaryotic AGOs has been...
HPF1-dependent histone ADP-ribosylation triggers chromatin relaxation to promote the recruitment of repair factors at sites of DNA damage [0.03%]
HPF1依赖的组蛋白ADP核糖化在DNA损伤位点募集修复因子促进染色质解压缩中发挥作用
Rebecca Smith,Siham Zentout,Magdalena Rother et al.
Rebecca Smith et al.
Poly(ADP-ribose) polymerase 1 (PARP1) activity is regulated by its co-factor histone poly(ADP-ribosylation) factor 1 (HPF1). The complex formed by HPF1 and PARP1 catalyzes ADP-ribosylation of serine residues of proteins near DNA breaks, mai...
Structural basis for TBP displacement from TATA box DNA by the Swi2/Snf2 ATPase Mot1 [0.03%]
SWI2/SNF2 型ATP酶 MOT1 从 TATA 盒DNA上置换 TBP 的结构基础
Stephan Woike,Sebastian Eustermann,James Jung et al.
Stephan Woike et al.
The Swi2/Snf2 family transcription regulator Modifier of Transcription 1 (Mot1) uses adenosine triphosphate (ATP) to dissociate and reallocate the TATA box-binding protein (TBP) from and between promoters. To reveal how Mot1 removes TBP fro...
Qu Chen,Rajesh P Menon,Laura Masino et al.
Qu Chen et al.
Immunoglobulin Fc receptors are cell surface transmembrane proteins that bind to the Fc constant region of antibodies and play critical roles in regulating immune responses by activation of immune cells, clearance of immune complexes and re...
Structural inventory of cotranslational protein folding by the eukaryotic RAC complex [0.03%]
模式生物RAC复合物 cotranslationally 折叠蛋白质的结构目录
Miglė Kišonaitė,Klemens Wild,Karine Lapouge et al.
Miglė Kišonaitė et al.
The challenge of nascent chain folding at the ribosome is met by the conserved ribosome-associated complex (RAC), which forms a chaperone triad with the Hsp70 protein Ssb in fungi, and consists of the non-canonical Hsp70 Ssz1 and the J doma...
Alberto García-Nieto,Amrita Patel,Yan Li et al.
Alberto García-Nieto et al.
In the early stages of mitosis, cohesin is released from chromosome arms but not from centromeres. The protection of centromeric cohesin by SGO1 maintains the sister chromatid cohesion that resists the pulling forces of microtubules until a...
Zhouyan Guo,Chen Chu,Yichen Lu et al.
Zhouyan Guo et al.
SIN3-HDAC (histone deacetylases) complexes have important roles in facilitating local histone deacetylation to regulate chromatin accessibility and gene expression. Here, we present the cryo-EM structure of the budding yeast SIN3-HDAC compl...