An intermediate state allows influenza polymerase to switch smoothly between transcription and replication cycles [0.03%]
介于复制和转录之间的中间状态使流感病毒聚合酶能够顺利切换复制和转录过程
Huanhuan Li,Yixi Wu,Minke Li et al.
Huanhuan Li et al.
Influenza polymerase (FluPol) transcribes viral mRNA at the beginning of the viral life cycle and initiates genome replication after viral protein synthesis. However, it remains poorly understood how FluPol switches between its transcriptio...
Daniel Wilinski,Monica Dus
Daniel Wilinski
Control of insulin mRNA translation is crucial for energy homeostasis, but the mechanisms remain largely unknown. We discovered that insulin mRNAs across invertebrates, vertebrates and mammals feature the modified base N6-methyladenosine (m...
A genome-wide screen reveals new regulators of the 2-cell-like cell state [0.03%]
全基因组筛选发现调控二细胞状态新因子
Nikhil Gupta,Lounis Yakhou,Julien Richard Albert et al.
Nikhil Gupta et al.
In mammals, only the zygote and blastomeres of the early embryo are totipotent. This totipotency is mirrored in vitro by mouse '2-cell-like cells' (2CLCs), which appear at low frequency in cultures of embryonic stem cells (ESCs). Because to...
Molecular basis for selective uptake and elimination of organic anions in the kidney by OAT1 [0.03%]
肾特异性有机阴离子的分子摄取和排泄机制OAT1
Joanne L Parker,Takafumi Kato,Gabriel Kuteyi et al.
Joanne L Parker et al.
In mammals, the kidney plays an essential role in maintaining blood homeostasis through the selective uptake, retention or elimination of toxins, drugs and metabolites. Organic anion transporters (OATs) are responsible for the recognition o...
The SUMO-NIP45 pathway processes toxic DNA catenanes to prevent mitotic failure [0.03%]
SUMO-NIP45信号通路加工有毒DNA联结复合物以防止有丝分裂失败
Emil P T Hertz,Ignacio Alonso-de Vega,Thomas Kruse et al.
Emil P T Hertz et al.
SUMOylation regulates numerous cellular processes, but what represents the essential functions of this protein modification remains unclear. To address this, we performed genome-scale CRISPR-Cas9-based screens, revealing that the BLM-TOP3A-...
Mechanistic and evolutionary insights into a type V-M CRISPR-Cas effector enzyme [0.03%]
V-M型CRISPR-Cas效应酶的机理和进化研究新进展
Satoshi N Omura,Ryoya Nakagawa,Christian Südfeld et al.
Satoshi N Omura et al.
RNA-guided type V CRISPR-Cas12 effectors provide adaptive immunity against mobile genetic elements (MGEs) in bacteria and archaea. Among diverse Cas12 enzymes, the recently identified Cas12m2 (CRISPR-Cas type V-M) is highly compact and has ...
Sperm chromatin structure and reproductive fitness are altered by substitution of a single amino acid in mouse protamine 1 [0.03%]
精氨酸单个氨基酸替代通过改变小鼠protamine 1的结构影响精子表观遗传状态和生育力
Lindsay Moritz,Samantha B Schon,Mashiat Rabbani et al.
Lindsay Moritz et al.
Conventional dogma presumes that protamine-mediated DNA compaction in sperm is achieved by electrostatic interactions between DNA and the arginine-rich core of protamines. Phylogenetic analysis reveals several non-arginine residues conserve...
Structural basis of sequence-specific cytosine deamination by double-stranded DNA deaminase toxin DddA [0.03%]
双链DNA脱氨酶毒素DddA特异性胞嘧啶去氨基的序列基础结构机制
Lulu Yin,Ke Shi,Hideki Aihara
Lulu Yin
The interbacterial deaminase toxin DddA catalyzes cytosine-to-uracil conversion in double-stranded (ds) DNA and enables CRISPR-free mitochondrial base editing, but the molecular mechanisms underlying its unique substrate selectivity have re...
Benjamin McIlwain
Benjamin McIlwain