PCNA recruits cohesin loader Scc2 to ensure sister chromatid cohesion [0.03%]
PCNA通过募集黏着素装载蛋白Scc2来保证姐妹染色单体的黏着
Ivan Psakhye,Ryotaro Kawasumi,Takuya Abe et al.
Ivan Psakhye et al.
Sister chromatid cohesion, established during replication by the ring-shaped multiprotein complex cohesin, is essential for faithful chromosome segregation. Replisome-associated proteins are required to generate cohesion by two independent ...
Simultaneous profiling of chromatin architecture and transcription in single cells [0.03%]
单细胞染色质构象与转录谱的同步分析
Jiale Qu,Jun Sun,Cai Zhao et al.
Jiale Qu et al.
The three-dimensional structure of chromatin plays a crucial role in development and disease, both of which are associated with transcriptional changes. However, given the heterogeneity in single-cell chromatin architecture and transcriptio...
Evaluating the role of the nuclear microenvironment in gene function by population-based modeling [0.03%]
基于群体的建模评估核微环境在基因功能中的作用
Asli Yildirim,Nan Hua,Lorenzo Boninsegna et al.
Asli Yildirim et al.
The nuclear folding of chromosomes relative to nuclear bodies is an integral part of gene function. Here, we demonstrate that population-based modeling-from ensemble Hi-C data-provides a detailed description of the nuclear microenvironment ...
Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development [0.03%]
泛康尼氏贫血DNA交联修复因子在小鼠发育期间保护LINE-1逆转录转座子入侵
Nazareno Bona,Gerry P Crossan
Nazareno Bona
Long interspersed nuclear element 1 (LINE-1) is the only autonomous retrotransposon in humans and new integrations are a major source of genetic variation between individuals. These events can also lead to de novo germline mutations, giving...
Magdalena Murawska,Andreas G Ladurner,Carla E Margulies
Magdalena Murawska
Frederic Zimmer,M Felicia Basilicata,Claudia Isabelle Keller Valsecchi
Frederic Zimmer
Droplet-based single-cell joint profiling of histone modifications and transcriptomes [0.03%]
基于液滴的组蛋白修饰和转录组单细胞联合谱型分析
Yang Xie,Chenxu Zhu,Zhaoning Wang et al.
Yang Xie et al.
We previously reported Paired-Tag, a combinatorial indexing-based method that can simultaneously map histone modifications and gene expression at single-cell resolution at scale. However, the lengthy procedure of Paired-Tag has hindered its...
Replication dynamics identifies the folding principles of the inactive X chromosome [0.03%]
复制动力学识别了失活X染色体的折叠原理
Rawin Poonperm,Saya Ichihara,Hisashi Miura et al.
Rawin Poonperm et al.
Chromosome-wide late replication is an enigmatic hallmark of the inactive X chromosome (Xi). How it is established and what it represents remains obscure. By single-cell DNA replication sequencing, here we show that the entire Xi is reorgan...