Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent RNA processing [0.03%]
核糖核酸转录及新生核糖核酸加工受到由dCas9介导的人类基因延伸障碍的调控
Inna Zukher,Gwendal Dujardin,Rui Sousa-Luís et al.
Inna Zukher et al.
Non-cleaving Cas9 (dCas9) is widely employed to manipulate specific gene loci, often with scant regard for unintended transcriptional effects. We demonstrate here that dCas9 mediates precise RNA polymerase II transcriptional pausing followe...
Structure and activation of the RING E3 ubiquitin ligase TRIM72 on the membrane [0.03%]
膜上TRIM72三聚复合物的结构与活化机制研究
Si Hoon Park,Juhyun Han,Byung-Cheon Jeong et al.
Si Hoon Park et al.
Defects in plasma membrane repair can lead to muscle and heart diseases in humans. Tripartite motif-containing protein (TRIM)72 (mitsugumin 53; MG53) has been determined to rapidly nucleate vesicles at the site of membrane damage, but the u...
Linna An,Derrick R Hicks,Dmitri Zorine et al.
Linna An et al.
In pseudocyclic proteins, such as TIM barrels, β barrels, and some helical transmembrane channels, a single subunit is repeated in a cyclic pattern, giving rise to a central cavity that can serve as a pocket for ligand binding or enzymatic...
Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD [0.03%]
活细胞三维单分子追踪揭示了NuRD对增强子动态的调节作用
S Basu,O Shukron,D Hall et al.
S Basu et al.
To understand how the nucleosome remodeling and deacetylase (NuRD) complex regulates enhancers and enhancer-promoter interactions, we have developed an approach to segment and extract key biophysical parameters from live-cell three-dimensio...
Cryo-EM structures of Myomaker reveal a molecular basis for myoblast fusion [0.03%]
冷冻电镜下Myomaker的结构揭示了肌母细胞融合的分子机制
Tao Long,Yichi Zhang,Linda Donnelly et al.
Tao Long et al.
The fusion of mononucleated myoblasts produces multinucleated muscle fibers leading to the formation of skeletal muscle. Myomaker, a skeletal muscle-specific membrane protein, is essential for myoblast fusion. Here we report the cryo-EM str...
Molecular mechanisms of inorganic-phosphate release from the core and barbed end of actin filaments [0.03%]
作用素丝状体中心和棒状末端无机磷酸根释放的分子机制
Wout Oosterheert,Florian E C Blanc,Ankit Roy et al.
Wout Oosterheert et al.
The release of inorganic phosphate (Pi) from actin filaments constitutes a key step in their regulated turnover, which is fundamental to many cellular functions. The mechanisms underlying Pi release from the core and barbed end of actin fil...
Time-resolved cryo-EM (TR-EM) analysis of substrate polyubiquitination by the RING E3 anaphase-promoting complex/cyclosome (APC/C) [0.03%]
RING型E3连接酶APC/C底物多泛素化的单颗粒低温电镜分析
Tatyana Bodrug,Kaeli A Welsh,Derek L Bolhuis et al.
Tatyana Bodrug et al.
Substrate polyubiquitination drives a myriad of cellular processes, including the cell cycle, apoptosis and immune responses. Polyubiquitination is highly dynamic, and obtaining mechanistic insight has thus far required artificially trapped...
PARylated PDHE1α generates acetyl-CoA for local chromatin acetylation and DNA damage repair [0.03%]
PARylation连接的PDHE1α产生乙酰辅酶A以用于局部染色质乙酰化和DNA损伤修复
Jun Zhang,Feng Chen,Yuan Tian et al.
Jun Zhang et al.
Chromatin relaxation is a prerequisite for the DNA repair machinery to access double-strand breaks (DSBs). Local histones around the DSBs then undergo prompt changes in acetylation status, but how the large demands of acetyl-CoA are met is ...
Aberrant gene activation in synovial sarcoma relies on SSX specificity and increased PRC1.1 stability [0.03%]
SSX特异性和PRC1.1稳定性的提高决定了滑膜肉瘤中基因激活异常
Nezha S Benabdallah,Vineet Dalal,R Wilder Scott et al.
Nezha S Benabdallah et al.
The SS18-SSX fusion drives oncogenic transformation in synovial sarcoma by bridging SS18, a member of the mSWI/SNF (BAF) complex, to Polycomb repressive complex 1 (PRC1) target genes. Here we show that the ability of SS18-SSX to occupy H2AK...