Molecular mechanism of glutaminase activation through filamentation and the role of filaments in mitophagy protection [0.03%]
谷氨酰胺酶通过丝状化激活的分子机制及其在线粒体自噬保护中的作用
Douglas Adamoski,Marilia Meira Dias,Jose Edwin Neciosup Quesñay et al.
Douglas Adamoski et al.
Glutaminase (GLS), which deaminates glutamine to form glutamate, is a mitochondrial tetrameric protein complex. Although inorganic phosphate (Pi) is known to promote GLS filamentation and activation, the molecular basis of this mechanism is...
Janice M Reimer,Andrea M Dickey,Yu Xuan Lin et al.
Janice M Reimer et al.
Leucine Rich Repeat Kinase 1 and 2 (LRRK1 and LRRK2) are homologs in the ROCO family of proteins in humans. Despite their shared domain architecture and involvement in intracellular trafficking, their disease associations are strikingly dif...
The modes of action of ion-channel-targeting neurotoxic insecticides: lessons from structural biology [0.03%]
离子通道靶向神经毒剂的作用方式：来自结构生物学的启示
Tobias Raisch,Stefan Raunser
Tobias Raisch
Insecticides are indispensable tools for plant protection in modern agriculture. Despite having highly heterogeneous structures, many neurotoxic insecticides use similar principles to inhibit or deregulate neuronal ion channels. Insecticide...
The substrate and inhibitor binding mechanism of polyspecific transporter OAT1 revealed by high-resolution cryo-EM [0.03%]
单颗粒冷冻电镜揭示多底物同向转运蛋白OAT1的底物和抑制剂结合机制
Tongyi Dou,Tengfei Lian,Shi Shu et al.
Tongyi Dou et al.
Organic anion transporters (OATs) of the SLC22 family have crucial roles in the transport of organic anions, including metabolites and therapeutic drugs, and in transporter-mediated drug-drug interactions. In the kidneys, OATs facilitate th...
Chiara Francavilla
Chiara Francavilla
Mario Leutert,Anthony S Barente,Noelle K Fukuda et al.
Mario Leutert et al.
The cellular ability to react to environmental fluctuations depends on signaling networks that are controlled by the dynamic activities of kinases and phosphatases. Here, to gain insight into these stress-responsive phosphorylation networks...
Shifted PAMs generate DNA overhangs and enhance SpCas9 post-catalytic complex dissociation [0.03%]
PAM错位生成DNA黏性末端并增强SpCas9酶解反应后的复合物解离
Jinglong Wang,Julien Le Gall,Richard L Frock et al.
Jinglong Wang et al.
Using Sanger sequencing and high-throughput genome sequencing of DNA cleavage reactions, we find that the Streptococcus pyogenes SpCas9 complex responds to internal mechanical strain by robustly generating a distribution of overhanging, rat...
Katarzyna Ciazynska
Katarzyna Ciazynska
Structural insights into an intracellular biased agonist for GPCRs [0.03%]
G蛋白偶联受体(GPCR)的偏向性胞内激动剂的结构研究
Neeraj Soni,Tarumoy Das,Mithu Baidya
Neeraj Soni
Structure of histone deacetylase complex Rpd3S bound to nucleosome [0.03%]
与核小体结合的组蛋白脱乙酰酶复合物Rpd3S的结构
Wulong Li,Hengjun Cui,Zhimin Lu et al.
Wulong Li et al.
Crosstalk between histone modifications represents a fundamental epigenetic mechanism in gene regulation. During the transcription elongation process, the histone deacetylase complex Rpd3S is recruited to H3K36-methylated nucleosomes to sup...