The p97/VCP adaptor UBXD1 drives AAA+ remodeling and ring opening through multi-domain tethered interactions [0.03%]
UBXD1通过多域相互作用驱动p97/VCP的AAA+重塑和环状结构打开
Julian R Braxton,Chad R Altobelli,Maxwell R Tucker et al.
Julian R Braxton et al.
p97, also known as valosin-containing protein, is an essential cytosolic AAA+ (ATPases associated with diverse cellular activities) hexamer that unfolds substrate polypeptides to support protein homeostasis and macromolecular disassembly. D...
Cryo-EM reveals how Hsp90 and FKBP immunophilins co-regulate the glucocorticoid receptor [0.03%]
冷冻电镜揭示Hsp90和FKBP免疫蛋白如何共同调控糖皮质激素受体的方式
Chari M Noddings,Jill L Johnson,David A Agard
Chari M Noddings
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of 'client' proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR t...
Large-scale, in-cell photocrosslinking at single-residue resolution reveals the molecular basis for glucocorticoid receptor regulation by immunophilins [0.03%]
大规模、单残基分辨率的细胞内光交联揭示免疫素对糖皮质激素受体调节的分子机制
Asat Baischew,Sarah Engel,Martha C Taubert et al.
Asat Baischew et al.
The Hsp90 co-chaperones FKBP51 and FKBP52 play key roles in steroid-hormone-receptor regulation, stress-related disorders, and sexual embryonic development. As a prominent target, glucocorticoid receptor (GR) signaling is repressed by FKBP5...
CRISPR-Cas9-based functional interrogation of unconventional translatome reveals human cancer dependency on cryptic non-canonical open reading frames [0.03%]
基于CRISPR-Cas9的功能性介入非传统转录组的研究揭示了人类癌症对隐秘的非 canonical 开放阅读框的依赖性
Caishang Zheng,Yanjun Wei,Peng Zhang et al.
Caishang Zheng et al.
Emerging evidence suggests that cryptic translation beyond the annotated translatome produces proteins with developmental or physiological functions. However, functions of cryptic non-canonical open reading frames (ORFs) in cancer remain la...
Ying Chen,Goran Kokic,Christian Dienemann et al.
Ying Chen et al.
Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to transcribing Pol II. The structures show that Elongi...
The molecular basis of drug selectivity for α5 subunit-containing GABAA receptors [0.03%]
含α5亚基的GABAA受体的选择性药物作用机制
Vikram Babu Kasaragod,Tomas Malinauskas,Ayla A Wahid et al.
Vikram Babu Kasaragod et al.
α5 subunit-containing γ-aminobutyric acid type A (GABAA) receptors represent a promising drug target for neurological and neuropsychiatric disorders. Altered expression and function contributes to neurodevelopmental disorders such as Dup1...
Martina Richeldi,Georgii Pobegalov,Torahiko L Higashi et al.
Martina Richeldi et al.
Cohesin forms a proteinaceous ring that is thought to link sister chromatids by entrapping DNA and counteracting the forces generated by the mitotic spindle. Whether individual cohesins encircle both sister DNAs and how cohesin opposes spin...
Fan Zhao,Chad W Hicks,Cynthia Wolberger
Fan Zhao
Monoubiquitination of histone H2B-K120/123 plays several roles in regulating transcription, DNA replication and the DNA damage response. The structure of a nucleosome in complex with the dimeric RING E3 ligase Bre1 reveals that one RING dom...