Protein destabilization underlies pathogenic missense mutations in ARID1B [0.03%]
ARID1B中致病错义突变的底层机制是蛋白质不稳定性的增加
Fanny Mermet-Meillon,Samuele Mercan,Beatrice Bauer-Probst et al.
Fanny Mermet-Meillon et al.
ARID1B is a SWI/SNF subunit frequently mutated in human Coffin-Siris syndrome (CSS) and it is necessary for proliferation of ARID1A mutant cancers. While most CSS ARID1B aberrations introduce frameshifts or stop codons, the functional conse...
Valeria Kalienkova,Mowgli Dandamudi,Cristina Paulino et al.
Valeria Kalienkova et al.
Rapid signaling between neurons is mediated by ligand-gated ion channels, cell-surface proteins with an extracellular ligand-binding domain and a membrane-spanning ion channel domain. The degenerin/epithelial sodium channel (DEG/ENaC) super...
Jinwoo Shin,Junhyeon Park,Jieun Jeong et al.
Jinwoo Shin et al.
Class C G-protein-coupled receptors (GPCRs) are activated through binding of agonists to the large extracellular domain (ECD) followed by rearrangement of the transmembrane domains (TMDs). GPR156, a class C orphan GPCR, is unique because it...
Structural mechanisms of autoinhibition and substrate recognition by the ubiquitin ligase HACE1 [0.03%]
泛素连接酶HACE1 自抑制及底物识别的结构机制
Jonas Düring,Madita Wolter,Julia J Toplak et al.
Jonas Düring et al.
Ubiquitin ligases (E3s) are pivotal specificity determinants in the ubiquitin system by selecting substrates and decorating them with distinct ubiquitin signals. However, structure determination of the underlying, specific E3-substrate comp...
Dynamic molecular architecture and substrate recruitment of cullin3-RING E3 ligase CRL3KBTBD2 [0.03%]
Cullin3-RING E3连接酶CRL3KBTBD2的动态分子结构和底物募集
Yuxia Hu,Zhao Zhang,Qiyu Mao et al.
Yuxia Hu et al.
Phosphatidylinositol 3-kinase α, a heterodimer of catalytic p110α and one of five regulatory subunits, mediates insulin- and insulin like growth factor-signaling and, frequently, oncogenesis. Cellular levels of the regulatory p85α subuni...
GPR161 structure uncovers the redundant role of sterol-regulated ciliary cAMP signaling in the Hedgehog pathway [0.03%]
GPR161结构揭示了固醇调控的纤毛cAMP信号在Hedgehog通路中的冗余作用
Nicholas Hoppe,Simone Harrison,Sun-Hee Hwang et al.
Nicholas Hoppe et al.
The orphan G protein-coupled receptor (GPCR) GPR161 plays a central role in development by suppressing Hedgehog signaling. The fundamental basis of how GPR161 is activated remains unclear. Here, we determined a cryogenic-electron microscopy...
Mechanism of millisecond Lys48-linked poly-ubiquitin chain formation by cullin-RING ligases [0.03%]
_RING E3泛连结酶毫秒级Lys48连接多泛素链的形成机制
Joanna Liwocha,Jerry Li,Nicholas Purser et al.
Joanna Liwocha et al.
E3 ubiquitin ligases, in collaboration with E2 ubiquitin-conjugating enzymes, modify proteins with poly-ubiquitin chains. Cullin-RING ligase (CRL) E3s use Cdc34/UBE2R-family E2s to build Lys48-linked poly-ubiquitin chains to control an enor...
A quantitative and site-specific atlas of the citrullinome reveals widespread existence of citrullination and insights into PADI4 substrates [0.03%]
精氨酸瓜氨酸化组的定量和位点特异性图谱揭示了广泛存在的瓜氨酸化及对PADI4底物的认识
Alexandra S Rebak,Ivo A Hendriks,Jonas D Elsborg et al.
Alexandra S Rebak et al.
Despite the importance of citrullination in physiology and disease, global identification of citrullinated proteins, and the precise targeted sites, has remained challenging. Here we employed quantitative-mass-spectrometry-based proteomics ...
Activation of CBASS Cap5 endonuclease immune effector by cyclic nucleotides [0.03%]
环核苷酸介导的CBASS免疫效应因子Cap5内切酶的激活
Olga Rechkoblit,Daniela Sciaky,Dale F Kreitler et al.
Olga Rechkoblit et al.
The bacterial cyclic oligonucleotide-based antiphage signaling system (CBASS) is similar to the cGAS-STING system in humans, containing an enzyme that synthesizes a cyclic nucleotide on viral infection and an effector that senses the second...