Lipid signaling to membrane proteins: From second messengers to membrane domains and adapter-free endocytosis [0.03%]
脂质信号与膜蛋白：从第二信使到膜结构域及非适应体依赖的内吞作用
Donald W Hilgemann,Gucan Dai,Anthony Collins et al.
Donald W Hilgemann et al.
Lipids influence powerfully the function of ion channels and transporters in two well-documented ways. A few lipids act as bona fide second messengers by binding to specific sites that control channel and transporter gating. Other lipids ac...
Estimating kinetic mechanisms with prior knowledge I: Linear parameter constraints [0.03%]
基于先验知识的动力学机制估算方法（一）：线性参数约束
Autoosa Salari,Marco A Navarro,Mirela Milescu et al.
Autoosa Salari et al.
To understand how ion channels and other proteins function at the molecular and cellular levels, one must decrypt their kinetic mechanisms. Sophisticated algorithms have been developed that can be used to extract kinetic parameters from a v...
Estimating kinetic mechanisms with prior knowledge II: Behavioral constraints and numerical tests [0.03%]
基于先验知识的 kinetics 机制估计 II：行为约束及数值测试
Marco A Navarro,Autoosa Salari,Mirela Milescu et al.
Marco A Navarro et al.
Kinetic mechanisms predict how ion channels and other proteins function at the molecular and cellular levels. Ideally, a kinetic model should explain new data but also be consistent with existing knowledge. In this two-part study, we presen...
Nonsensing residues in S3-S4 linker's C terminus affect the voltage sensor set point in K+ channels [0.03%]
S3-S4连接体C端的非感应残基影响K+通道的电压传感器设定点
Joao L Carvalho-de-Souza,Francisco Bezanilla
Joao L Carvalho-de-Souza
Voltage sensitivity in ion channels is a function of highly conserved arginine residues in their voltage-sensing domains (VSDs), but this conservation does not explain the diversity in voltage dependence among different K+ channels. Here we...
A model-free method for measuring dimerization free energies of CLC-ec1 in lipid bilayers [0.03%]
一种测量脂质双层中CLC-EC1二聚化自由能的无模型方法
Rahul Chadda,Lucy Cliff,Marley Brimberry et al.
Rahul Chadda et al.
The thermodynamic reasons why membrane proteins form stable complexes inside the hydrophobic lipid bilayer remain poorly understood. This is largely because of a lack of membrane-protein systems amenable for equilibrium studies and a limite...
The relationship between form and function throughout the history of excitation-contraction coupling [0.03%]
兴奋收缩偶联历史上形式和功能的关系
Clara Franzini-Armstrong
Clara Franzini-Armstrong
The concept of excitation-contraction coupling is almost as old as Journal of General Physiology It was understood as early as the 1940s that a series of stereotyped events is responsible for the rapid contraction response of muscle fibers ...
Molecular determinants of pH regulation in the cardiac Na+-Ca2+ exchanger [0.03%]
心脏Na+-Ca2+交换器pH调节的分子决定因素
Scott John,Brian Kim,Riccardo Olcese et al.
Scott John et al.
The cardiac Na+-Ca2+ exchanger (NCX) plays a critical role in the heart by extruding Ca2+ after each contraction and thus regulates cardiac contractility. The activity of NCX is strongly inhibited by cytosolic protons, which suggests that i...
Junctional trafficking and restoration of retrograde signaling by the cytoplasmic RyR1 domain [0.03%]
胞浆型RyR1在半通道转运和恢复逆向信号传导中的作用
Alexander Polster,Stefano Perni,Dilyana Filipova et al.
Alexander Polster et al.
The type 1 ryanodine receptor (RyR1) in skeletal muscle is a homotetrameric protein that releases Ca2+ from the sarcoplasmic reticulum (SR) in response to an "orthograde" signal from the dihydropyridine receptor (DHPR) in the plasma membran...
ATP is stored in lamellar bodies to activate vesicular P2X4 in an autocrine fashion upon exocytosis [0.03%]
ATP以自分泌方式储存在板层体内，在出胞后通过激活囊泡型P2X4被活化
Giorgio Fois,Veronika Eva Winkelmann,Lara Bareis et al.
Giorgio Fois et al.
Vesicular P2X4 receptors are known to facilitate secretion and activation of pulmonary surfactant in the alveoli of the lungs. P2X4 receptors are expressed in the membrane of lamellar bodies (LBs), large secretory lysosomes that store lung ...
Small Ca2+ releases enable hour-long high-frequency contractions in midshipman swimbladder muscle [0.03%]
少量钙离子释放使刺尾鱼游泳膀胱肌肉产生高频收缩数小时
Frank E Nelson,Stephen Hollingworth,James O Marx et al.
Frank E Nelson et al.
Type I males of the Pacific midshipman fish (Porichthys notatus) vibrate their swimbladder to generate mating calls, or "hums," that attract females to their nests. In contrast to the intermittent calls produced by male Atlantic toadfish (O...