Increased EThcD efficiency on the hybrid Orbitrap Excedion Pro Mass Analyzer extends the depth in identification and sequence coverage of HLA class I immunopeptidomes [0.03%]
基于杂交Orbitrap Excedium Pro质谱分析仪的EThcD效率提升扩展了HLA-I类免疫肽组鉴定和序列覆盖率的深度
Amy L Kessler,Kyle L Fort,Hanno C Resemann et al.
Amy L Kessler et al.
Gaining a complete and unbiased understanding of the non-tryptic peptide repertoire presented by HLA-I complexes by LC-MS/MS is indispensable for therapy design for cancer, autoimmunity and infectious diseases. A serious concern in HLA pept...
Proteome and secretome profiling of the melanoma-induced transition towards immune incompetent dendritic cells reveals enhanced IDO1, cathepsin, and legumain activity [0.03%]
黑色素瘤诱导的免疫无能树突细胞转变的蛋白质组和分泌组分析揭示了IDO1、半胱天冬酶和芸香糖活性增强
Anouk M D Becker,Bob J Ignacio,Jelmer J Dijkstra et al.
Anouk M D Becker et al.
Dendritic cells (DCs) are professional antigen-presenting cells endowed with the capacity to initiate strong antitumor immune responses. This function is critical for effective DC-based immunotherapies but is often hampered by tumor-derived...
Corrigendum to "Comprehensive Proteomics Metadata and Integrative Web Portals Facilitate Sharing and Integration of LINCS Multiomics Data" [0.03%]
“综合的蛋白质组学元数据和集成网络门户可促进LINCS多组学数据的共享与整合”一文勘误
Dušica Vidović,Behrouz Shamsaei,Stephan C Schürer et al.
Dušica Vidović et al.
Localisation of Organelle Proteins using Data-Independent Acquisition (DIA-LOP) [0.03%]
基于数据非独立获取的细胞器蛋白定位技术(DIA-LOP)
K J A McCaskie,C Hutchings,R Feret et al.
K J A McCaskie et al.
Subcellular localisation within the proteome fundamentally influences cellular processes, however the development of high-throughput techniques to allow proteome-wide mapping of the cell has proven difficult. Here we present DIA-LOP, an app...
From Vision to Reality: A Perspective on the Innovative and Collaborative Forces that Evolved Proximity Labeling [0.03%]
从愿景到现实：关于邻近标记技术的创新及合作力量的观点叙述
Tess C Branon,Wei Qin,Namrata D Udeshi
Tess C Branon
Proximity labeling coupled to mass spectrometry is a powerful method for studying living systems with high spatial and temporal resolution. This perspective aims to provide a history of how APEX and TurboID-based proximity labeling technolo...
Structural Insights into Complement Inhibition: Visualizing Distinct Binding Modes of C4b-binding Protein Complexes with C4b and SAP [0.03%]
补体抑制的结构见解：C4b结合蛋白与C4b和SAP复合物的多种结合模式的可视化研究
Tereza Kadavá,Jürgen Strasser,Maryam Marefat et al.
Tereza Kadavá et al.
C4b-binding protein (C4BP) is an innate immune inhibitor found in serum. Human C4BP adopts spider-like higher-order structures (HOS) formed by disulfide-linked C4BPα and C4BPβ chains that non-covalently bind vitamin K-dependent protein S ...
High-throughput proteomic and phosphoproteomic analysis of formalin-fixed paraffin-embedded tissues [0.03%]
甲醛固定石蜡包埋组织的高通量蛋白质组和磷酸化蛋白质组分析
Moe Haines,John R Thorup,Simone Gohsman et al.
Moe Haines et al.
Formalin-fixed, paraffin-embedded (FFPE) patient tissues are a valuable resource for proteomic studies with the potential to associate derived molecular insights with clinical annotations and outcomes. Here we present an optimized, partiall...
Proteomic Analysis of Hsp90β-selective Inhibitors Against Triple-negative Breast Cancer (TNBC) to Gain a Mechanistic Insight [0.03%]
热休克蛋白90β选择性抑制剂作用于三阴性乳腺癌的蛋白质组学分析及机制研究
Tyelor S Reynolds,Daniel Hu,Simon Weaver et al.
Tyelor S Reynolds et al.
Hsp90 is a central molecular chaperone responsible for the folding and activation of >400 client proteins, causing it to be a highly sought after drug target for the treatment of cancer. Hsp90 pan-inhibitors have been evaluated in the clini...
Exploring virus-host interactions through combined proteomic approaches identifies BANF1 as a new essential factor for African Swine Fever Virus [0.03%]
整合蛋白质组学方法鉴定出BANF1是非洲猪瘟病毒的新型宿主限制因子
Juliette Dupré,Katarzyna Magdalena Dolata,Gang Pei et al.
Juliette Dupré et al.
African swine fever virus (ASFV) causes a lethal disease in pigs and represents a significant threat to the global pork industry due to the lack of effective vaccines or treatments. Despite intensive research, many ASFV proteins remain unch...
An automated workflow to address proteome complexity and the large search space problem in proteomics and HLA-I immunopeptidomics [0.03%]
针对蛋白质组复杂性和大型搜索空间问题的自动化工作流程在蛋白质组学和HLA-I免疫肽组学中的应用
Yehor Horokhovskyi,Hanna P Roetschke,John A Cormican et al.
Yehor Horokhovskyi et al.
Antigenic noncanonical epitope and novel protein discovery are research areas with therapeutical applications, predominantly done via mass spectrometry. The latter should rely on a well-characterized proteogenomic search space. Its size is ...