Single Cell Proteomics Reveals Specific Cellular Subtypes in Cardiomyocytes Derived from Human iPSCs and Adult Hearts [0.03%]
单细胞蛋白质组揭示由人类多能干细胞和成人心脏衍生的心肌细胞中特定的细胞亚型
Lizhuo Ai,Aleksandra Binek,Vladimir Zhemkov et al.
Lizhuo Ai et al.
Single cell proteomics was performed on human induced pluripotent stem cells (iPSCs), iPSC-derived cardiomyocytes, and adult cardiomyocytes. Over 700 proteins could be simultaneously measured in each cell revealing unique subpopulations. A ...
Filter-aided extracellular vesicle enrichment (FAEVEr) for proteomics [0.03%]
用于蛋白质组学的滤助细胞外囊泡富集(FAEVEr)法
Jarne Pauwels,Tessa Van de Steene,Jana Van de Velde et al.
Jarne Pauwels et al.
Extracellular vesicles (EVs), membrane-delimited nanovesicles that are secreted by cells into the extracellular environment, are gaining substantial interest due to their involvement in cellular homeostasis and their contribution to disease...
Site-Specific Competitive Kinase Inhibitor Target Profiling Using Phosphonate Affinity Tags [0.03%]
基于焦磷酸盐亲和标签的位点特异性竞争性激酶抑制剂靶标分析方法
Wouter van Bergen,Anneroos E Nederstigt,Albert J R Heck et al.
Wouter van Bergen et al.
Protein kinases are prime targets for drug development due to their involvement in various cancers. However, selective inhibition of kinases, while avoiding off-target effects remains a significant challenge for the development of protein k...
Causal Inference and Annotation of Phosphoproteomics Data in Multi-omics Cancer Studies [0.03%]
多组学癌症研究中的磷酸蛋白质组数据的因果推理与标注
Qun Dong,Minjia Tan,Yingchun Zhou et al.
Qun Dong et al.
Protein phosphorylation plays a crucial role in regulating diverse biological processes. Perturbations in protein phosphorylation are closely associated with downstream pathway dysfunctions, while alterations in protein expression could ser...
High-throughput determination of exchange rates of unmodified and PTM-containing peptides using HX-MS [0.03%]
基于HX-MS的未修饰及翻译后修饰肽段交换速率的高通量测定方法
Jamie Moroco,Alvaro Sebastian Vaca Jacome,Pierre Michel Jean Beltran et al.
Jamie Moroco et al.
Despite the widespread use of MS for hydrogen/deuterium exchange measurements, no systematic, large-scale study has been conducted to compare the observed exchange rates in protein-derived, unstructured peptides measured by MS to the predic...
Improving the Depth and Reliability of Glycopeptide Identification Using Protein Prospector [0.03%]
利用蛋白质Prospector提高糖肽鉴定的深度和可靠性
Robert J Chalkley,Peter R Baker
Robert J Chalkley
Glycosylation is the most common and diverse modification of proteins. It can affect protein function and stability and is associated with many diseases. While proteomic methods to study most post-translational modifications are now quite m...
Molecular Display of the Animal Meta-Venome for Discovery of Novel Therapeutic Peptides [0.03%]
动物宏基因组分子展示技术在新型治疗性多肽发现中的应用
Meng-Hsuan Hsiao,Yang Miao,Zixing Liu et al.
Meng-Hsuan Hsiao et al.
Animal venoms, distinguished by their unique structural features and potent bioactivities, represent a vast and relatively untapped reservoir of therapeutic molecules. However, limitations associated with comprehensively constructing and ex...
Multi-tiered proteome analysis displays the hyper-permeability of the rheumatoid synovial compartment for plasma proteins [0.03%]
多层蛋白组分析显示类风湿关节滑膜腔对血浆蛋白质的高通透性
Eva Maria Stork,Sofia Kalaidopoulou Nteak,Danique M H van Rijswijck et al.
Eva Maria Stork et al.
Rheumatoid arthritis (RA) is characterized by synovial hyperplasia and cartilage/bone destruction. RA affects the synovial joints, the synovial lining and the permeability of the synovium. As the latter is of central relevance for the distr...
Early life exposure to deltamethrin impairs synaptic function by altering the brain derived extracellular vesicle proteome [0.03%]
德尔塔锰醇早期暴露通过改变脑源性细胞外囊泡蛋白组损害突触功能
L Koff,J Di Re,S Chand et al.
L Koff et al.
Pyrethroid pesticides have been associated with neurodevelopmental disorders including attention deficit hyperactivity disorder (ADHD) and autism spectrum disorder (ASD). While behavioral effects of pyrethroid exposure have been previously ...
Sensitive and accurate proteome profiling of embryogenesis using Real-Time Search and TMTproC quantification [0.03%]
使用实时搜索和TMTproC定量进行灵敏且精确的胚胎发生蛋白质组分析
Alex N T Johnson,Jingjing Huang,Argit Marishta et al.
Alex N T Johnson et al.
Multiplexed proteomics has become a powerful tool for investigating biological systems. Using balancer-peptide conjugates (e.g., TMTproC complementary ions) in the MS2 spectra for quantification circumvents the ratio distortion problem inhe...