Age-related changes in relative expression of real-time PCR housekeeping genes in human skeletal muscle [0.03%]
人骨骼肌实时PCR管家基因相对表达量的年龄相关变化
Chad D Touchberry,Michael J Wacker,Scott R Richmond et al.
Chad D Touchberry et al.
The purpose of this investigation was to examine the expression of three commonly used housekeeping genes -- glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta(2)-microglobulin (beta(2)M), and RNA polymerase 2a (polR2a) -- in elderly (E...
Protein fragment domains identified using 2D gel electrophoresis/MALDI-TOF [0.03%]
利用二维凝胶电泳/基质辅助激光解析离子化法鉴定蛋白质片段的结构域
Maria D Person,Jianjun Shen,Angelina Traner et al.
Maria D Person et al.
We previously reported a protein expression profiling experiment conducted on human pancreatic tissues using 2D gel electrophoresis and mass spectrometry. Here, 18 spots that were identified in the gel at molecular weights more than 10 kDa ...
Evaluation of methods for sequence analysis of highly repetitive DNA templates [0.03%]
高重复序列DNA模板的测序方法评估
John W Hawes,Kevin L Knudtson,Helaman Escobar et al.
John W Hawes et al.
The DNA Sequencing Research Group (DSRG) of the ABRF conducted a study to assess the ability of DNA sequencing core facilities to successfully sequence a set of well-defined templates containing difficult repeats. The aim of this study was ...
Multicenter Study
Journal of biomolecular techniques : JBT. 2006 Apr;17(2):138-44. DOI: 2006
Validation of a reversed-phase HPLC method for quantitative amino acid analysis [0.03%]
反相HPLC定量氨基酸分析方法的验证
Maria Paola Bartolomeo,Federico Maisano
Maria Paola Bartolomeo
A semi-automated method for amino acid derivatization and analysis has been validated for use in analysis of protein biopharmaceuticals. The method includes protein hydrolysis, o-phthalaldehyde derivatization, and reversed-phase high-perfor...
Signal and sensitivity enhancement through optical interference coating for DNA and protein microarray applications [0.03%]
用于DNA和蛋白质微阵列的光学干涉膜的信号及灵敏度增强作用研究
Rajendra J Redkar,Niko A Schultz,Volker Scheumann et al.
Rajendra J Redkar et al.
Optical inteference (OI) coated slides with unique optical properties were utilized in microarray analyses, demonstrating their enhanced detection sensitivity over traditional microarray substrates. The OI coating is comprised of a propriet...
Metal ion-mediated reduction in surface entropy improves diffraction quality of crystals of the IRAK-4 death domain [0.03%]
金属离子介导的表面熵减少可改善IRAK-4死亡结构城晶体的衍射质量
Michael V Lasker,Santosh M Kuruvilla,Mark M Gajjar et al.
Michael V Lasker et al.
Interleukin-1 receptor-associated kinase-4 (IRAK-4) is an essential component of innate immunity in mice and humans. IRAK-4 is a bipartite protein composed of a death domain (DD) that mediates molecular recognition, and a catalytic kinase d...
Identification of the DNA bases of a DNase I footprint by the use of dye primer sequencing on an automated capillary DNA analysis instrument [0.03%]
利用自动毛细管DNA分析仪和染料引物对DNase I保护序列中的DNA碱基进行鉴定
Michael Zianni,Kimberly Tessanne,Massimo Merighi et al.
Michael Zianni et al.
We have adapted the techniques of DNA footprint analysis to an Applied Biosystems 3730 DNA Analyzer. The use of fluorescently labeled primers eliminates the need for radioactively labeled nucleotides, as well as slab gel electrophoresis, an...
The wildcat toolbox: a set of perl script utilities for use in peptide mass spectral database searching and proteomics experiments [0.03%]
野猫工具箱：一组用于肽质量矩阵数据库搜索及蛋白质组学实验的PERL脚本实用程序
Paul A Haynes,Susan Miller,Tim Radabaugh et al.
Paul A Haynes et al.
We describe in this communication a set of functional perl script utilities for use in peptide mass spectral database searching and proteomics experiments, known as the Wildcat Toolbox. These are all freely available for download from our l...
Horst Will,Matthias Dettloff,Peter Bendzkô et al.
Horst Will et al.
Aggrecanase activities of ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) proteinases were measured with a recombinant aggrecan fragment and two monoclonal antibodies. Recombinant human aggrecan interglobular domain ...
Alan L Rockwood,David K Crockett,James R Oliphant et al.
Alan L Rockwood et al.
Many recent advances in biology and medicine have resulted from DNA sequence alignment algorithms and technology. Traditional approaches for the matching of DNA sequences are based either on global alignment schemes or heuristic schemes tha...