Analysis of one-step and two-step real-time RT-PCR using SuperScript III [0.03%]
SuperScript III进行一步法和两步法实时RT-PCR的分析比较
Michael J Wacker,Michael P Godard
Michael J Wacker
Real-time reverse transcription polymerase chain reaction (RT-PCR) is a commonly used technique to analyze gene expression. There has been little research conducted to test if SuperScript III quantitative one-step (reverse transcription car...
Assessment of some tools for the characterization of the human osteoarthritic cartilage proteome [0.03%]
评估用于表征人类骨关节炎软骨蛋白质组的一些工具
Frédéric De Ceuninck,Estelle Marcheteau,Sylvie Berger et al.
Frédéric De Ceuninck et al.
Since the proteome of osteoarthritic articular cartilage has been poorly investigated as yet, we adapted proteomic technologies to the study of the proteins secreted or released by fresh human osteoarthritic cartilage in culture. Fresh cart...
Kathryn M Ivanetich,Wilson Yan,Kathleen M Wunderlich et al.
Kathryn M Ivanetich et al.
The quantification of plasmid DNA by the PicoGreen dye binding assay has been automated, and the effect of quantification of user-submitted templates on DNA sequence quality in a core laboratory has been assessed. The protocol pipets, mixes...
Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein [0.03%]
利用T4噬菌体基因32蛋白优化体外转录和全长cDNA合成
Caroline Piché,Johann P Schernthaner
Caroline Piché
We evaluated the effect of the T4 bacteriophage gene 32 protein (T4gp32) on in vitro transcription and reverse transcription. T4gp32 doubled the yield of in vitro transcripts obtained with T7 RNA polymerase and increased the yield of cDNA s...
Monitoring the disruption of nuclear envelopes in interphase cells with GFP-beta-galactosidase [0.03%]
用GFP-β半乳糖苷酶监测分裂间期细胞核膜的破裂现象
Leticia Sánchez,Mohamed Kodiha,Ursula Stochaj
Leticia Sánchez
The nuclear envelope of eukaryotic cells provides a barrier separating nucleus from cytoplasm, thereby regulating the exchange of macromolecules between both compartments. However, in cells exposed to severe forms of stress this barrier may...
Nucleic acid aptamers for target validation and therapeutic applications [0.03%]
核酸适配体在靶标确证和治疗应用中的作用
P Shannon Pendergrast,H Nicholas Marsh,Dilara Grate et al.
P Shannon Pendergrast et al.
In the simplest view, aptamers can be thought of as nucleic acid analogs to antibodies. They are able to bind specifically to proteins, and, in many cases, that binding leads to a modulation of protein activity. New aptamers are rapidly gen...
Simple modifications of the standard DNA sequencing protocol allow for sequencing through siRNA hairpins and other repeats [0.03%]
对标准DNA测序协议进行简单的修改可实现siRNA发夹和其他重复序列的测序
Jan Kieleczawa
Jan Kieleczawa
RNAi is a relatively new but powerful technology used for monitoring gene silencing in many species. The RNA corresponding to the gene of interest can be delivered into cells using various protocols, but the approach where both sense and an...
Characterization of protein glycosylation using chip-based nanoelectrospray with precursor ion scanning quadrupole linear ion trap mass spectrometry [0.03%]
基于芯片的纳喷雾结合前体离子扫描四极杆线性阱质谱分析糖蛋白糖链的方法学研究
Sheng Zhang,Brian L Williamson
Sheng Zhang
Glycosylation is one of the most important posttranslational modifications affecting the functions of proteins and cell activities. Mass spectrometry (MS) has proven to be an effective tool for structural glycobiology and has helped gain an...
Screening for transglutaminase-catalyzed modifications by peptide mass finger printing using multipoint recalibration on recognized peaks for high mass accuracy [0.03%]
利用已鉴定肽峰多点校正的肽质量指纹图谱法筛选转谷氨酰胺酶催化修饰产物
Cecilia Sundby Emanuelsson,Sandor Boros,Karin Hjernoe et al.
Cecilia Sundby Emanuelsson et al.
Detection of posttranslational modifications is expected to be one of the major future experimental challenges for proteomics. We describe herein a mass spectrometric procedure to screen for protein modifications by peptide mass fingerprint...