Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein
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We evaluated the effect of the T4 bacteriophage gene 32 protein (T4gp32) on in vitro transcription and reverse transcription. T4gp32 doubled the yield of in vitro transcripts obtained with T7 RNA polymerase and increased the yield of cDNA synthesis when used i... ...
