Mutually Activated Dual-Exponential Amplification DNA Machine Enhances Robust CRISPR/Cas12a Feedback Propagation for Ultrasensitive miRNA Detection [0.03%]
互激活的双指数放大DNA机器增强CRISPR / Cas12a反馈传播以实现超灵敏miRNA检测
Qi Wang,Shuo Yang,Xiumei Chen et al.
Qi Wang et al.
Breast cancer (BC) remains a critical global health challenge, necessitating ultrasensitive methods for detecting biomarkers such as miR-155, a key regulator in BC progression. Here, we present a mutually activated dual-exponential amplific...
A Method for Determination of Transport Efficiency in Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Tissue Analysis [0.03%]
用于组织激光剥蚀电感耦合等离子体质谱分析的传输效率测定方法
Jaromír Stráník,Vilém Svojanovský,Julie Weisová et al.
Jaromír Stráník et al.
Recent studies have demonstrated the applicability of the "single particle" mode in laser ablation-inductively coupled plasma-mass spectrometry to map and size particles simultaneously. The transport efficiency (TE) is an important paramete...
The Impact of Molecular Symmetry and Rigidity on the Selective Analysis of VOCs by Mid-IR Laser Spectroscopy [0.03%]
分子对称性和刚性对中红外激光光谱选择性分析挥发性有机物的影响研究
Miloš Selaković,Renato Zenobi,Lukas Emmenegger et al.
Miloš Selaković et al.
This study highlights the potential of mid-infrared laser absorption spectroscopy (LAS) for analyzing volatile organic compounds (VOCs) in the gas phase. Using a Vernier-type quantum cascade laser (QCL) in the 9-10 μm spectral region, over...
Sugar Analysis Using Hydrophilic Liquid Chromatography Combined with Raman Spectroscopy [0.03%]
基于亲水作用色谱与拉曼光谱联用技术的糖分析方法研究
Yu-Sheng Chen,Hirotsugu Hiramatsu
Yu-Sheng Chen
The combination of chromatography and Raman spectroscopy is challenging because the eluate flows fast and limits the accumulation time of weak Raman signals. The total reflection of light in a liquid core waveguide (LCW) can enhance the Ram...
In-Line Enrichment of Cancer Cells from Whole Blood by Cell Self-Organization in Acoustic Fields [0.03%]
声场中细胞自组织用于全血癌症细胞的富集分离
Richard Soller,Ola Jakobsson,Per Augustsson
Richard Soller
We present a novel method for in-line acoustofluidic separation of cancer cells directly from whole blood. We report for the first time the observation that blood cells can self-organize when packed closely together in an acoustic field. We...
A Programmable Dynamic Range Electrochemiluminescence Platform with Dual-Signal Output and Single Luminophore, Utilizing Polyadenine-Anchored Triblock DNA [0.03%]
一种基于多信号输出和单发光团的聚腺苷酸锚定三嵌段DNA的可编程电致化学发光平台
Yang Jiang,Chunjia Ren,Yanxia Qiao et al.
Yang Jiang et al.
Rational design of nucleic acid capture probes to modulate binding affinity is a valuable strategy for programing the dynamic range and limit of detection (LOD) in electrochemiluminescence (ECL) assays. Current strategies, however, have sca...
Label-Free Quantification of Apoptosis and Necrosis Using Stimulated Raman Scattering Microscopy [0.03%]
基于受激发拉曼散射显微镜的细胞凋亡和坏死无标记定量研究
Shivam Mahapatra,Shreya B Shivpuje,Helen C Campbell et al.
Shivam Mahapatra et al.
Recombinant proteins are critical for modern therapeutics and diagnostics, with Chinese hamster ovary (CHO) cells serving as the primary production platform. However, environmental and chemical stressors in bioreactors often trigger cell de...
Miniaturized 3D-Printed Multipass Helmholtz Photoacoustic Sensors for High-Sensitivity CO2 Detection with Near-Infrared (NIR) Diode Lasers [0.03%]
用于高灵敏度二氧化碳检测的微型化多通道赫姆霍兹光声传感器与近红外（NIR）激光器搭配使用
Ruyue Cui,Yupeng Yuan,Sicheng Qui et al.
Ruyue Cui et al.
Monitoring carbon dioxide (CO2) concentrations is crucial for environmental protection and various industrial applications. This study presents a high-sensitivity photoacoustic spectroscopy (PAS) sensing system based on an innovative 3D-pri...
Active Learning Improves Ionization Efficiency Predictions and Quantification in Nontargeted LC/HRMS [0.03%]
主动学习提高非目标LC/HRMS离子化效率预测和定量能力
Wei-Chieh Wang,Nahid Amini,Carolin Huber et al.
Wei-Chieh Wang et al.
Liquid chromatography electrospray ionization high-resolution mass spectrometry (LC/ESI/HRMS) is frequently employed in nontargeted screening (NTS) due to its high selectivity and sensitivity. However, data interpretation is challenging sin...
Controllable Ion-Repulsion Enables Rapid and Trace-Level Detection of Hydrophobic Sulfonylurea Herbicides with ICPMS/MS without the Organic Mode [0.03%]
可控离子排斥实现在无机模式下用ICPMS/MS快速灵敏检测磺酰脲除草剂
Bassam Lajin,Walter Goessler
Bassam Lajin
The inductively coupled plasma tandem mass spectrometry (ICPMS/MS) is emerging as a detector for compounds tagged with a nonmetal, but can be hampered by poor compatibility with organic eluents. Herein, we describe an alternative approach t...