Imaging of Protein Assemblies up to 231 kDa in Tissues with Nano-DESI Mass Spectrometry [0.03%]
纳米DESI质谱成像蛋白质复合物质量高达231kDa
Oliver J Hale,Helen J Cooper
Oliver J Hale
Understanding the distribution of proteins and their assemblies in tissues is a major challenge in spatial biology. Mass spectrometry imaging (MSI) with nanospray-desorption electrospray ionization (nano-DESI) has previously enabled detecti...
VirMolAnalyte: An AI-Driven In Silico Metabolite Annotation Tool [0.03%]
基于人工智能的代谢产物注释工具VirMolAnalyte
Guilin Hu,Jameel Hizam Alafifi,Minghua Qiu
Guilin Hu
Metabolites play a crucial role in sustaining biological activities and are also a significant source of new drug development. Nuclear magnetic resonance (NMR) spectroscopy is one of the most important tools for identifying the structures o...
A Simple Online Internal Standard Calibration Strategy for Single-Particle Inductively Coupled Plasma Mass Spectrometry Based on Multielement Analysis [0.03%]
基于多元素分析的单颗粒电感耦合等离子体质谱内标校准策略
Xiangwei Tian,Ying Wang,Peijie Yang et al.
Xiangwei Tian et al.
Single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS) is a powerful tool for resolving particle heterogeneity. However, in real environmental and biological samples, matrix effects pose significant challenges to accurate ...
Stimuli-Responsive Fusion of Binary-Star Intelligent DNA System for the Signal-On Amplification Detection of Cancer-Related miRNA [0.03%]
基于智能DNA二元星体系的刺激响应型信号放大检测癌症相关microRNA方法
Liujing Pan,Yao Wu,Wenhao Pan et al.
Liujing Pan et al.
While extensive efforts have been devoted toward multifunctional DNA assembly-based systems for imaging intracellular miRNAs, challenges remain in clinical translation due to their inherently vulnerable degradability and poor assay sensitiv...
Online Electroextraction-NanoESI-MS for Rapid Analysis of Catecholamines in Urine [0.03%]
基于电渗超液相-纳喷雾串联质谱的尿儿茶酚胺在线分析方法研究
Meng Zhang,Yanxiao Jiang,Qiong Wu et al.
Meng Zhang et al.
The direct mass spectrometric analysis of complex biological samples is often hindered by matrix interference and the need for extensive sample preparation. In this study, an online electroextraction method based on nano-electrospray mass s...
Fluorinated Side-Chain-Modified Peptides Inspired by Shark Skin for Antifouling Electrochemical Biosensing: Ultrasensitive Detection of CA15-3 in Human Saliva [0.03%]
受鲨鱼皮启发的含氟侧链修饰多肽在抗污染电化学生物传感中的应用：人体唾液中CA15-3的超灵敏检测
Kunpeng Yu,Xin Sun,Zhen Song et al.
Kunpeng Yu et al.
A major challenge limiting the practical application of electrochemical biosensors is biofouling in complex biological environments, leading to poor signal strength, reduced operational stability, and thereby impaired practical utility. One...
Enhanced Sensitivity and Resolution in Biomolecular CEST NMR Experiments Using the Extended Hadamard Encoding Scheme [0.03%]
扩展的哈达玛编码方案在生物分子CEST核磁共振实验中增强灵敏度和分辨率
Jihyun Kim,Micael Silva,E̅riks Kupče et al.
Jihyun Kim et al.
By untangling information using deterministic frequency-domain linear combinations, the Hadamard Transform (HT) offers a robust way to extract spectral information. Hadamard-based encoding schemes can shorten the acquisition times of nuclea...
Pure Shift 2D and 1D NMR in Tabular Domain via Complete Reduction to Amplitude Frequency Table [CRAFTps] [0.03%]
基于完整缩减到振幅频率表的纯位移二维和一维核磁共振光谱(CRAFTps)
Krish Krishnamurthy,Dan Iverson
Krish Krishnamurthy
Pure shift spectra significantly improve the resolution in NMR by collapsing all multiplet signals into singlets. Homonuclear BB decoupling is typically accomplished by selective inversion of passive spins midway between dwell points or chu...
High-Wavenumber Raman Spectroscopy for the Identification and Biochemical Characterization of Microbial Species [0.03%]
用于微生物鉴定和生化表征的高波数拉曼光谱学
Alec B Walter,Ezekiel Haugen,Anna S Rourke-Funderburg et al.
Alec B Walter et al.
Raman spectroscopy is a powerful tool for microbiological and infectious disease research, enabling rapid discrimination of microbial species. While spectral discrimination has typically been performed using the fingerprint region (400-1800...
AND-Gate Logic-Controlled Catalytic Hybridization Network for Robust Dual-Mode Fluorescence-Colorimetric Detection of Breast Cancer miRNAs [0.03%]
一种用于乳腺癌miRNA robust双模式荧光/比色检测的AND门逻辑控制催化杂交网络
Yao Yao,Shuyan Chen,Yujun Cheng et al.
Yao Yao et al.
Given the low abundance of circulating miRNAs and the complexity of serum matrices, the simultaneous liquid biopsy of multiple miRNAs with both high sensitivity and accuracy remains a formidable challenge. To address these limitations, we d...