Chromatography-Free Analysis of Mixtures Using a Two-Dimensional Mass Spectrometry (2DMS)-Enabled Quadrupole Time-of-Flight (QToF) Analyzer [0.03%]
无须色谱的混合物分析及其使能的二维质谱(2DMS)-四极杆飞行时间(QToF)分析仪
Steven Wright,Nathan Cassidy,Alex Colburn et al.
Steven Wright et al.
Two-dimensional mass spectrometry (2DMS) is a powerful data-independent analysis (DIA) technique that allows mixtures to be analyzed without chromatographic separation of the components. Until now, high-resolution 2DMS has only been possibl...
Dual-Response and Dual-Targeting Probe Based on the Quinolinothiazole Salt Capable of Decoding Cellular Stress and Cellular State [0.03%]
一种基于喹啉并噻唑盐的双响应和双靶向探针用于解码细胞应激和细胞状态
Hong-Di Xu,Qi Lin,Wen-Pei Ni et al.
Hong-Di Xu et al.
The functional state of subcellular organelles served as a fundamental indicator of the cellular health. However, conventional fluorescent probes were largely limited to single-parameter detection, failing to capture coordinated interorgane...
A Disposable CRISPR-Nanozyme Electrochemical Biosensor for Rapid and Sensitive Detection of Breast Cancer Circulating Tumor DNA [0.03%]
一种用于快速灵敏检测乳腺癌循环肿瘤DNA的CRISPR-纳米酶电化学生物传感器
Xiaofang Liu,Fan Shi,Guangli Luo et al.
Xiaofang Liu et al.
Circulating tumor DNA (ctDNA) serves as a promising next-generation biomarker for noninvasive cancer screening and monitoring. In this work, we report an electrochemical biosensor for ctDNA detection. The sensor is constructed based on the ...
Quantification of Ingredients and Additives of Beer and Beer-Based Mixed Beverages by 1H NMR Spectroscopy and Automated PULCON Analysis [0.03%]
基于~(1)H核磁共振谱的啤酒及啤酒预调酒中原料与添加剂定量分析方法研究
Sabine Milbert,Jakob T Laumeyer,Teresa M Müller et al.
Sabine Milbert et al.
In Germany, traditional beer consumption is declining, while the demand for nonalcoholic and beer-based mixed drinks is increasing. International regulations on ingredient labeling, particularly for additives and sweeteners, challenge consu...
Interfacial Hydrogen Bond-Mediated Rigidification Strategy Coupled with Stepwise Antenna Effect for Ultrasensitive Electrochemiluminescence Detection [0.03%]
界面氢键刚性化策略结合电致化学发光检测中的阶梯天线效应用于超灵敏检测
Ziyu Zhao,Xueling Shan,Yuqi Wu et al.
Ziyu Zhao et al.
Lanthanide metal organic frameworks (Ln-MOFs) are promising electrochemiluminescence (ECL) emitters due to their unique lanthanide antenna effect. However, the vibrational modes of organic ligands significantly induce nonradiative transitio...
Glucose/O2 Enzymatic Biofuel Cell Constructed with a Laccase-Mimicking Nanozyme for Efficient Cathode Oxygen Reduction and Bacterial Surface-Displayed Cascade Enzymes for an Anode Biocatalyst [0.03%]
一种基于模拟漆酶的纳米酶的葡萄糖/氧型 enzymatic biofuel cell 的阴极催化剂及用于阳极生物催化剂的细菌表面展示级联系统酶
Yuhan Chen,Boyan Wang,Yafei Du et al.
Yuhan Chen et al.
Enzymatic biofuel cell (EBFC) is an electrochemical device that uses enzymes to convert the chemical energy of renewable biomass into electrical energy. However, improving the EBFC performance, such as the cost-effectiveness, operational st...
High-Throughput Quantification of Spike Protein Expression by LC-MS for mRNA-LNP Evaluation and Immune Response Profiling [0.03%]
基于LC-MS的高通量Spike蛋白表达定量方法用于mRNA脂质纳米粒评价及免疫反应谱型分析
Ruben Almey,Kevin Mwangi,Amélie De Maesschalck et al.
Ruben Almey et al.
The COVID-19 pandemic has accelerated interest in immuno-multiple reaction monitoring (immuno-MRM) for peptide quantification, with early efforts focusing on SARS-CoV-2 biomarker detection in clinical nasopharyngeal swabs. However, the emer...
Combining Engineered Probe Library with Tiered Screening for the Rational Discovery of Alzheimer's Diagnostic Probe with an Enhanced Signal-to-Noise Ratio [0.03%]
结合工程探针文库与分级筛选合理发现阿尔茨海默诊断探针并增强信噪比
Zhenpeng Li,Xueqian Chen,Jiatian Liu et al.
Zhenpeng Li et al.
Alzheimer's disease (AD) is neuropathologically defined by the deposition of β-amyloid (Aβ) plaques, a key diagnostic biomarker. However, current fluorescence probes targeting Aβ plaques are often limited by poor blood-brain barrier (BBB...
Ionic Liquid-Regulated Interfacial Microenvironment Enables Highly Efficient Electrochemiluminescence [0.03%]
离子液体调控界面微环境实现高效的电致化学发光反应
Yichen Li,Rui Zou,Weijiang Guan et al.
Yichen Li et al.
The efficiency of electrochemiluminescence (ECL) is often limited by insufficient electron transfer and reactant accumulation on electrode surfaces. Herein, tunable ionic liquid (IL)-based microdomains were assembled on gold electrodes to e...
A Programmable Y-Shaped Catalytic Hairpin Assembly Amplification Enabling Ultrasensitive Fluorescent Analysis of Circular RNAs in Living Cells [0.03%]
一种可编程的Y型催化发夹组装扩增方法实现活细胞中环形RNA的超灵敏荧光分析
Ying Li,Wenbo Lu,Wei-Guo Yang et al.
Ying Li et al.
Circular RNAs (circRNAs) have become a research hotspot due to their high stability, tissue specificity, and disease-regulating effects. Among them, circRNA mitochondrial tRNA translation optimization 1 (circMTO1) is abnormally expressed in...