Jaymi January,Kaylin Januarie,Nelia Sanga et al.
Jaymi January et al.
Determination of Cellular Oxygen Consumption Rate Based on Phosphorescence Intensity Measurements Using a Blue-Emitting Hydrophilic Ir(III) Complex [0.03%]
基于蓝光发射亲水型铱(III)配合物氧合物磷光强度测定细胞耗氧率
Mari Seno,Shuichi Shiozaki,Toshitada Yoshihara
Mari Seno
Intracellular oxygen (O2) is closely associated with cellular metabolism, as represented by energy production, and the cellular O2 consumption rate (OCR) in particular serves as a valuable indicator of metabolic activity. Here, we developed...
Hierarchically Structured Functionalized Polymer Monoliths Via Two-Photon Polymerization for Miniaturized HPLC Separation Columns [0.03%]
基于双光子聚合的分层结构功能化整体柱材料用于高效液相色谱分离微通道
Tobias Drieschner,Tobias Werres,Martin Klassen et al.
Tobias Drieschner et al.
Miniaturization of liquid chromatography (LC) systems offers numerous advantages. Nevertheless, miniaturized LC is still not widely used. Here, we present the additive manufacturing of hierarchically structured macro- and mesoporous functio...
Fluorescent Peptide Probe for Specific Detection and In Vivo Imaging of Fusobacterium Nucleatum [0.03%]
荧光肽探针用于特定检测和活体成像检测福赛氏菌
Hang Wu,Buyue Zhang,Ziqiang Lu et al.
Hang Wu et al.
Fusobacterium nucleatum (F. nucleatum), an opportunistic oral anaerobic pathogen, is closely associated with various oral disorders and colorectal cancer (CRC). Current detection methods for this bacterium suffer from technical complexity a...
A Multi-Analyte-Responsive, Multi-Organ-Imaging, Dual-NIR-Emissive Fluorescent Probe for Visualizing Pyroptosis-Mediated Hg2+-Induced Hepatorenal Dysfunction [0.03%]
一种多参数响应、多器官成像的近红外发射荧光探针：用于可视化由细胞焦亡介导的Hg2+诱导的肝肾功能障碍
Zibo Zhai,Liang Tao,Jia Huang et al.
Zibo Zhai et al.
Mercury ions (Hg2+) are highly toxic heavy metals that are widely distributed in the environment and pose a serious threat to both ecosystems and human health. After entering the body, mercury preferentially accumulates in the liver and kid...
Integrated Low-Speed Isolation and Ultrasensitive In-Situ Detection of Glioblastoma EVs via UiO-66-NH2@Au Nanocomposite with Temperature Synergistic Membrane Fusion and Y-EXPAR Assay [0.03%]
基于UiO-66-NH2@Au纳米复合材料的温度协同膜融合与Y-EXPAR检测低速隔离胶质母细胞瘤外泌体集成芯片传感器及其超灵敏原位检测
Yan-Yan Chen,Xue-Xian Deng,Yan Wang et al.
Yan-Yan Chen et al.
Extracellular vesicles (EVs) secreted by glioblastoma (GBM) cells carry tumor-specific biomarkers such as microRNA-1246 (miR-1246), offering significant potential for noninvasive GBM detection. However, the efficient isolation of EVs from c...
ApuShape: Human-in-the-Loop Annotation Software for Fluorescent Nuclei Segmentation [0.03%]
基于荧光染色的细胞核分割的人机交互式标注软件APUShape
Ziwei Chen,Jingyi Li,Qiushi Wei et al.
Ziwei Chen et al.
Large-scale cellular imaging studies increasingly require highly accurate and efficient nucleus annotation in fluorescence microscopy images. However, dedicated annotation software designed for high-throughput nucleus instance segmentation ...
Analyte-Specific "Signal-On" Uranyl Sensitive Sensing Based on Temperature-Programmed Nanozyme Activity of CoOx [0.03%]
基于CoOx程序升温纳米酶活性的特异性“信号开启”尿式传感检测法
Zhijian Bu,Linjie Wang,Yuxuan Yao et al.
Zhijian Bu et al.
As alternatives to bioenzymes, nanozymes find promising applications in biochemical sensing thanks to their recognized superiorities, but their lack of specific interactions with targets of interest seriously hinders the exploration of sele...
Heterojunction-Mediated Dual-Site Synergistic Catalysis for Enhanced Electrochemiluminescence Detection of α-Synuclein [0.03%]
异质结介导的双活性位点协同催化用于α-突触核蛋白电致化学发光检测增强研究
Yamei Li,Xue Dong,Tingting Wu et al.
Yamei Li et al.
Precise quantification of α-synuclein (α-Syn), an important species associated with Parkinson's disease and related neurodegenerative disorders, is of paramount importance for early diagnosis and intervention. While electrochemiluminescen...
Dual-pH LC-ESI-MS/MS Expands Peptide Detectability via Wrong-Way-Round Ionization [0.03%]
基于反向离子化的 Dual-pH 检测方法可提高肽段检测覆盖率
Ola Svahn,Jonatan Svahn,Jan Astermark
Ola Svahn
Quantitative LC-ESI-MS/MS methods for peptides are conventionally developed and performed under acidic mobile-phase conditions to favor protonation and stable precursor formation. However, despite this long-standing practice, an increasing ...