High-Throughput Single-Cell Proteomics Enabled by Integrating nPOP Workflow with Quantitative Hyperplexing [0.03%]
基于nPOP工作流与定量超复用技术的高通量单细胞蛋白质组学研究
Kunpei Cai,Qing Zeng,Chuanxi Huang et al.
Kunpei Cai et al.
Single-cell proteomics (scProteomics) has emerged as a powerful approach to dissect cellular heterogeneity and dynamic molecular mechanisms at unprecedented resolution. However, achieving high proteome coverage and quantitative accuracy whi...
Characterizing the Influence of Filter Substrates on Raman Measurements of Microplastics [0.03%]
滤料对微塑料拉曼光谱测量影响的研究
Shiwani Shiwani,Ines Latka,S M Miftahul Islam et al.
Shiwani Shiwani et al.
Raman spectroscopy is a powerful tool for the characterization of microplastics in environmental samples; however, measurements are typically performed directly on filter substrates, making substrate selection a critical factor for reliable...
Tandem MICROFASP Method-Enabled High-Sensitivity In Vivo Cross-Linking Mass Spectrometry Analysis of Low-Cell-Number Samples without Enrichment [0.03%]
无需富集的低细胞数样本高灵敏度活体交联质谱分析的新方法：串联MICROFASP法
Yu He,Yang Li,Rong Liu et al.
Yu He et al.
Cross-linking mass spectrometry (XL-MS) has emerged as an indispensable tool for dissecting protein conformations and protein-protein interactions (PPIs) within native biological systems. However, conventional in vivo XL-MS workflows suffer...
Hydrogen Carrier Gas Method Translation in Comprehensive Two-Dimensional Gas Chromatography for Sustainable Nontargeted Analysis [0.03%]
氢载气转换技术在可持续非目标综合二维气相色谱分析中的应用
Kira M Fisher,Emma L Macturk,Katelynn A Perrault Uptmor
Kira M Fisher
The selection and control of carrier gas are critical factors influencing the performance of both one-dimensional gas chromatography (1D GC) and comprehensive two-dimensional gas chromatography (GC×GC). Helium is widely used due to its ine...
Enzyme-Activated Near-Infrared Fluorescent Probe for Rapid and Precise Identification of Staphylococcus aureus-Induced Skin Infections [0.03%]
一种用于快速、精准识别金黄色葡萄球菌诱导皮肤感染的酶激活近红外荧光探针
Junchao Xu,Yong Tan,Yuan Ye et al.
Junchao Xu et al.
Staphylococcus aureus (S. aureus) is a major opportunistic pathogen responsible for diverse skin and soft tissue infections, which may rapidly progress to severe or systemic infections if not promptly managed. Therefore, the rapid and preci...
Electrochemiluminescence Imaging Digital Immunoassay of Cytokines Secreted by Activated T Cells [0.03%]
活化T细胞分泌细胞因子的电致化学发光成像数字免疫测定法
Yajuan Yan,Jialian Ding,Tengyu Li et al.
Yajuan Yan et al.
High-sensitivity cytokine detection is essential for predicting immunotherapy efficacy and monitoring treatment. Conventional methods such as ELISA suffer from limited sensitivity, while single-molecule immunoassays, although highly sensiti...
Biopanning of Peptides Specific to the Conserved Epitope of the SARS-CoV-2 Receptor-Binding Domain and Nanozyme-Assisted Dual Peptide-Based Sandwich ELISA for Ultrasensitive Detection of the SARS-CoV-2 Antigen [0.03%]
针对SARS-CoV-2受体结合域保守表位的肽生物淘选及纳米酶辅助双肽夹心ELISA法超灵敏检测SARS-CoV-2抗原
Mingyang Wang,Haipeng Yu,Wanjian Liu et al.
Mingyang Wang et al.
As the subunit of the SARS-CoV-2 spike protein (SARS-CoV-2 SP), the receptor-binding domain (RBD) can specifically bind to human angiotensin-converting enzyme 2 (ACE2), enabling viral infection of host cells. Therefore, it is of utmost sign...
Potential-Resolved Electrochemiluminescence via Interfacial Electronic Engineering in Au25@FeMOP Heterostructures for Intelligent Urine Analysis [0.03%]
基于界面电子工程的Au25@FeMOP异质结构在电致化学发光中的应用及其在智能尿检中的应用研究
Huining Chai,Xiao Tan,Xi Sun et al.
Huining Chai et al.
Precise modulation of reactive oxygen species (ROS) pathways is pivotal for advanced electrochemiluminescence (ECL) sensing. However, achieving this in complex biofluids remains a formidable challenge. Herein, we report a hierarchical hollo...
Light-Regulated Iontronic Sensing for Resolving the Charge-Steric Trade-Off in the Detection of Exosomal m5C-Modified miR-21 [0.03%]
光调节离子传感技术解决游离外泌体m5C修饰miR-21检测的电荷-立体位阻权衡问题
Baojing Jiang,Tianyi Lu,Yanlei Li et al.
Baojing Jiang et al.
Exosomal microRNA methylation represents a critical epigenetic modification closely implicated in cancer progression. However, its sensitive detection continues to pose substantial challenges, owing to the inherent coupling between charge a...
Unraveling CTC Heterogeneity: DLD-Assisted Immunomagnetic Sorting and ICP-MS for the Isolation and Profiling of Epithelial-Mesenchymal Transition-Related States [0.03%]
揭示CTC异质性：采用DLD辅助免疫磁分选和ICP-MS技术对上皮间质转化相关状态进行分离与表征
Junrong Ma,Man He,Beibei Chen et al.
Junrong Ma et al.
Accurate analysis of circulating tumor cell (CTC) heterogeneity at the single-cell level, in addition to circulating tumor cell (CTC) enumeration, holds great promise for advancing our understanding of cancer progression and therapeutic res...