Comparative thermodynamic and kinetic properties governing the nucleic acid interactions of CRISPR-Cas9 and Cas12a [0.03%]
CRISPR-Cas9和Cas12a与核糖核酸相互作用的热力学和动力学性质的比较研究
Camila Molina,Alexa L Knight,George P Lisi
Camila Molina
Clustered Regularly Interspaced Short Palindromic Repeat-associated proteins (CRISPR-Cas) biochemistry has been leveraged in genome editing applications in biochemical research and therapeutics. CRISPR-Cas9 and CRISPR-Cas12a are the two mos...
Liquid-liquid phase separation induces stochastic oscillations in gene regulation [0.03%]
液滴凝聚诱导基因表达的随机振荡现象
Lijun Hong,Zhenquan Zhang,Zihao Wang et al.
Lijun Hong et al.
Rhythmic gene expression underlies core physiological processes across organisms, from circadian timekeeping to stress responses. Recent experiments suggest that the regulation of such rhythmic dynamics involves protein compartmentalisation...
Mechanistic modeling predicts efficacy of CISH knockout in tumor-infiltrating lymphocytes with synergistic gene editing [0.03%]
机制建模预测CISH敲除在肿瘤浸润淋巴细胞中的疗效及基因编辑的协同效应
Nikolaos Memmos,Kamran Kaveh,Beau R Webber et al.
Nikolaos Memmos et al.
Tumor-infiltrating lymphocyte (TIL) therapy is a type of adoptive cell therapy, where the lymphocytes of a cancer patient's tumor are harvested, expanded in vitro using IL-2 stimulation, and then infused back into the patient[1], [2]. Howev...
Rapid and interpretable protein contact map prediction using a pattern-matching strategy [0.03%]
基于模式匹配策略的快速且可解释的蛋白质接触图预测方法
Aysima Hacisuleyman,Dirk Fasshauer
Aysima Hacisuleyman
Protein sequence determines structure, function, and dynamics, yet the gap between sequenced proteins and experimentally determined structures continues to widen. While machine learning approaches like AlphaFold2 have transformed structural...
Thermodynamic uncertainty relation constraints information transmission through cell signaling systems [0.03%]
热力学不确定性关系约束细胞信号系统中的信息传递
Shreyansh Verma,Vishva Saravanan R,Bhaswar Ghosh
Shreyansh Verma
Biological systems in general operates out of equilibrium which demands the requirement for constant supply of energy due to non-equilibrium entropy production. The thermodynamic uncertainty relation (TUR) essentially imposes a bound on min...
The Gaussian Network Model as a framework for allosteric analysis: dynamic distance, edge centrality, and entropy sensitivity in KRAS [0.03%]
高斯网络模型作为别构分析的框架：KRAS中的动态距离、边中心性和熵敏感性
Burak Erman
Burak Erman
Allosteric communication in proteins relies on network connectivity patterns that channel conformational signals between distant sites. We introduce a unified mathematical framework based on three complementary measures of network organizat...
Molecular modeling of the orphan SLC6A16 transporter revealed an unusual composition of the substrate transport pathway [0.03%]
孤儿SLC6A16转运蛋白的分子建模揭示了底物运输途径的独特组成
Tolith Gidaga,Jędrzej Kukułowicz,Martyna Ogos et al.
Tolith Gidaga et al.
SLC6A16 (NTT5) is a poorly understood member of the solute carrier 6 (SLC6) family, a group of sodium-dependent transporters that shuttle amino acids and monoamines across cell membrane. While many SLC6 transporters have been well character...
Identifying the sources of noise synergy and redundancy in the gene expression of feed-forward loop motif [0.03%]
识别串阶式回路模式基因表达中的噪声协同作用和冗余的来源
Mintu Nandi,Sudip Chattopadhyay,Suman Kumar Banik
Mintu Nandi
The propagation of noise through parallel pathways is a characteristic feature of feed-forward loops (FFLs) in genetic networks. Although the contributions of the direct and indirect pathways to output variability have been well characteriz...
Physical and chemical considerations for successful in vitro culture of rust fungi: challenges, insights and novel strategies [0.03%]
绣病菌离体培养的物理和化学因素：挑战、见解与新策略
Sarah Sale,Volker Nock,Ashley Garrill
Sarah Sale
Rust fungi cause significant economic and biodiversity losses worldwide, yet effective control strategies for them remain limited. A major challenge in identifying control targets is the inability to culture them through the different stage...
Olamide Ishola,Adeyemi Ogunbowale,Emma Abdul-Rahman et al.
Olamide Ishola et al.
Biological membranes define cellular and organelle boundaries, and perform vital functions, providing transport, recognition, signaling, and interaction with other cells. These membranes are majorly composed of lipid bilayers and membrane p...