Universal Improvement of In Situ Hybridization Chain Reaction by Reducing Background Signals Caused by Single Probes [0.03%]
通过减少单个探针引起的背景信号普遍改善原位放大杂交反应
Yudai Kuboe,Kensuke Nakanishi,Ichiro Tazawa et al.
Yudai Kuboe et al.
The in situ hybridization chain reaction (HCR) method involves designing multiple target sequences and a pair of split probes for each target. One split probe contains the complementary sequence for half of the target along with part of the...
Yui Uchida,Tasuku Ishida,Satomi Ono et al.
Yui Uchida et al.
The official poster for the First Evo-Devo Young Researchers Meeting. It features the meeting title, themes ("Evo-Devo so far" and "Evo-Devo in the future"), invited speakers, program highlights, and logistical details. The content is writt...
GLI3 in Archaic Humans: Possible Contributions to Human Skeletal and Brain Evolution [0.03%]
尼安德特人中的GLI3基因：可能对人类骨骼和大脑进化的贡献
Tadashi Nomura,Ako Agata,Nguyen Thi My Trinh
Tadashi Nomura
Neanderthals, an extinct hominid, lived in Eurasia until about 40,000 years ago. According to fossils, Neanderthals had distinctive anatomical features compared to modern humans, including a long front-to-back cranium, low frontal bones, an...
Syncytin-1 Is Responsible for the Fusion Between Human Trophoblasts and Endometrial Stromal Cells [0.03%]
合体滋养层细胞和子宫内膜基质细胞融合由Syncytin-1介导
Akira Oike,Shun Shibata,Takahiro Arima et al.
Akira Oike et al.
Syncytiotrophoblasts (STs) are multinucleated cells formed by the fusion of trophoblasts and play critical roles in placental development and function. Retrovirus-derived fusogenic proteins, known as syncytins, regulate trophoblast fusion b...
Transcriptome Analysis Suggested Striking Transition Around the End of Epiboly in the Gene Regulatory Network Downstream of the Oct4-Type POU Gene in Zebrafish Embryos [0.03%]
转录组分析揭示斑马鱼胚胎Oct4型POU基因下游基因调控网络在表层期结束时出现显著转换
Masaaki Ikeda,Kana Kobayashi,Yukiko Nakayama-Sadakiyo et al.
Masaaki Ikeda et al.
Zebrafish pou5f3 encodes a Class V POU transcription factor, Pou5f3, which regulates various developmental processes, including neurogenesis and brain formation. In the current study, we attempted to comprehensively identify the Pou5f3 down...
Step-by-Step Protocol for Making a Knock-In Xenopus laevis to Visualize Endogenous Gene Expression [0.03%]
体内目的基因表达可视化爪蟾敲入载体构建及应用的方案步骤
Norie Kagawa,Yoshihiko Umesono,Ken-Ichi T Suzuki et al.
Norie Kagawa et al.
We established a novel knock-in technique, New and Easy Xenopus Targeted integration (NEXTi), to recapitulate endogenous gene expression by reporter expression. NEXTi is a CRISPR-Cas9-based method to integrate a donor DNA containing a repor...
Shoma Kuriyama,Keisuke Shigematsu,Seung June Kwon et al.
Shoma Kuriyama et al.
Snakes serve as important models for understanding how changes in genes and genome sequences drive vertebrate morphological evolution. However, the lack of established primary culture methods and gene delivery techniques for snake cells has...
Measuring intraventricular pressure in developing mouse embryos: Uncovering a repetitive mechanical cue for brain development [0.03%]
发育小鼠胚胎脑室内压测定：揭示了大脑发育的重复机械线索
Mami Akaike,Jun Hatakeyama,Yuta Nakashima et al.
Mami Akaike et al.
In living organisms, including humans, the developmental processes that construct their morphology from a single fertilized egg are influenced not only by genetic regulation but also by various external factors. One such factor is mechanica...
The long-term survival of enteroendocrine cells depends on their subtype and is linked to peripheral sensory innervation [0.03%]
肠内分泌细胞的长期生存取决于其亚型，并与外周感觉神经支配有关
Salsabila Luthfi Sesotyosari,Masato Kinoshita,Mukhamad Sunardi et al.
Salsabila Luthfi Sesotyosari et al.
Enteroendocrine cells (EECs) are sensory epithelial cells that sense the gut luminal environment and convey sensory information to the brain via the visceral afferent pathway. Although EECs are a part of gut epithelial cells, which generall...
Waves of innovation and collaboration: Zebrafish research converges in Kyoto [0.03%]
创新与合作的浪潮：京都斑马鱼研究汇聚点
Takuya Kaneko
Takuya Kaneko
The 18th International Zebrafish Conference "IZFC2024" was held in Kyoto from August 17 to 21, 2024. © 2025 Japanese Society of Developmental Biologists...