Transcriptome Analysis Suggested Striking Transition Around the End of Epiboly in the Gene Regulatory Network Downstream of the Oct4-Type POU Gene in Zebrafish Embryos [0.03%]
转录组分析揭示斑马鱼胚胎Oct4型POU基因下游基因调控网络在表层期结束时出现显著转换
Masaaki Ikeda,Kana Kobayashi,Yukiko Nakayama-Sadakiyo et al.
Masaaki Ikeda et al.
Zebrafish pou5f3 encodes a Class V POU transcription factor, Pou5f3, which regulates various developmental processes, including neurogenesis and brain formation. In the current study, we attempted to comprehensively identify the Pou5f3 down...
Step-by-Step Protocol for Making a Knock-In Xenopus laevis to Visualize Endogenous Gene Expression [0.03%]
体内目的基因表达可视化爪蟾敲入载体构建及应用的方案步骤
Norie Kagawa,Yoshihiko Umesono,Ken-Ichi T Suzuki et al.
Norie Kagawa et al.
We established a novel knock-in technique, New and Easy Xenopus Targeted integration (NEXTi), to recapitulate endogenous gene expression by reporter expression. NEXTi is a CRISPR-Cas9-based method to integrate a donor DNA containing a repor...
Shoma Kuriyama,Keisuke Shigematsu,Seung June Kwon et al.
Shoma Kuriyama et al.
Snakes serve as important models for understanding how changes in genes and genome sequences drive vertebrate morphological evolution. However, the lack of established primary culture methods and gene delivery techniques for snake cells has...
Measuring intraventricular pressure in developing mouse embryos: Uncovering a repetitive mechanical cue for brain development [0.03%]
发育小鼠胚胎脑室内压测定：揭示了大脑发育的重复机械线索
Mami Akaike,Jun Hatakeyama,Yuta Nakashima et al.
Mami Akaike et al.
In living organisms, including humans, the developmental processes that construct their morphology from a single fertilized egg are influenced not only by genetic regulation but also by various external factors. One such factor is mechanica...
The long-term survival of enteroendocrine cells depends on their subtype and is linked to peripheral sensory innervation [0.03%]
肠内分泌细胞的长期生存取决于其亚型，并与外周感觉神经支配有关
Salsabila Luthfi Sesotyosari,Masato Kinoshita,Mukhamad Sunardi et al.
Salsabila Luthfi Sesotyosari et al.
Enteroendocrine cells (EECs) are sensory epithelial cells that sense the gut luminal environment and convey sensory information to the brain via the visceral afferent pathway. Although EECs are a part of gut epithelial cells, which generall...
Waves of innovation and collaboration: Zebrafish research converges in Kyoto [0.03%]
创新与合作的浪潮：京都斑马鱼研究汇聚点
Takuya Kaneko
Takuya Kaneko
The 18th International Zebrafish Conference "IZFC2024" was held in Kyoto from August 17 to 21, 2024. © 2025 Japanese Society of Developmental Biologists...
Efficient CRISPR/Cas9-mediated knockin of reporter genes in rats at ROSA26 by pronuclear microinjection [0.03%]
高效ROSALIND-CRISPR-CAS9介导的报道基因敲入大鼠的方法
Takaya Abe,Ken-Ichi Inoue,Hiroshi Kiyonari
Takaya Abe
The genetic modification of rats is a key technology for advancing biomedical research on human diseases. CRISPR/Cas9-mediated genome editing enables the generation of knockout rats in a single step, without the need for embryonic stem cell...
Integrative approaches in Xenopus-based research: A report from the 1st Asian Xenopus Conference [0.03%]
基于非洲爪蟾研究的整合方法：来自第一届亚洲非洲爪蟾会议的报告
Dong Gil Jang,Hyo Jung Sim,Keun Yeong Kwon
Dong Gil Jang
The first Asian community conference on "Scientific research using Xenopus as an animal model system" was held in Osaka, Japan, from November 24 to 26, 2024. This event was organized by the Korean Society for Xenopus Development of KSMCB (K...
Tadashi Nomura,Chiaki Ohtaka-Maruyama
Tadashi Nomura
The mammalian neocortex is characterized by tangential surface expansion and a six-layer layered structure. However, developmental mechanisms underlying the evolution of the neocortex remain to be elucidated. We hold the symposium entitled ...
Chisaki Shima,Ayaka Ohashi,Saya Furukawa et al.
Chisaki Shima et al.
As skin ages, its structure and function undergo significant transformations driven by complex cellular and molecular processes. In this study, we explore these changes using the axolotl, an amphibian model known for its transparent skin, a...