Step-by-Step Protocol for Making a Knock-In Xenopus laevis to Visualize Endogenous Gene Expression
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We established a novel knock-in technique, New and Easy Xenopus Targeted integration (NEXTi), to recapitulate endogenous gene expression by reporter expression. NEXTi is a CRISPR-Cas9-based method to integrate a donor DNA containing a reporter gene (egfp) into... ...
