A Single-Chain Light-Activatable Transcriptional Reporter for Fluorescently Tagging Mammalian Cells In Vitro [0.03%]
一种单链光激活转录报告基因，用于标记体外哺乳动物细胞荧光素標签
Ola Bartolik,Wenjing Wang
Ola Bartolik
Optogenetic tools have revolutionized the control of gene expression with high spatial and temporal resolution. Here we present a Single-chain Light-Activatable Transcriptional Reporter (SLATR), a system capable of fluorescently tagging tar...
Synthesis of Polyhydroxylated Pyrrolidinyl Analogs of Sugar Nucleotides. Experimental and Computational Studies on Their Interactions With GalNAC-T's [0.03%]
糖核苷酸多羟基化吡咯烷类似物的合成及其与GalNAc-Ts相互作用的实验和计算研究
Pedro Merino,Veronica Juste,Alejandro Montesa et al.
Pedro Merino et al.
Direct coupling between polyhydroxylated pyrrolidinyl β-amino phosphonates and uridine monophosphate (UMP) yields polyhydroxylated pyrrolidinyl analogs of sugar nucleotides suitable inhibitors of N-acetyl-galactosyltransferases. β-Amino p...
Enzyme-Assisted Synthesis and In Vitro Characterization of Bifunctional PCSK9 Inhibitors [0.03%]
酶促合成及体外表征双功能PCSK9抑制剂
Yuhui Zhang,Li Wang,Leo Corcilius et al.
Yuhui Zhang et al.
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a well-established target for lowering cholesterol and is abundantly present in the extracellular space. Inhibitors of PCSK9 have achieved marked success in the clinic, but an alterna...
Force-Forster Resonance Energy Transfer Correlation Microscopy: A Multimodal Approach for the Study of Single-Nucleotide-Resolution Ribosome Translocation [0.03%]
用于研究单核糖体沿mRNA相对移动的多模态方法
Mariah Noggler,Shawonur Rahaman,Yuhong Wang et al.
Mariah Noggler et al.
Force spectroscopy and Förster resonance energy transfer (FRET) are both widely applicable techniques for biomolecular research. Each has its own advantages and also limitations, such as the interference of nonspecific binding for force sp...
Elevating Haloperoxidase Expression in Escherichia coli through Fusion with a Formate Oxidase [0.03%]
通过与甲酸氧化酶融合提高大肠杆菌中过氧合酶的表达
Angelique Pothuizen,Jacob M A van Hengst,Ron Wever et al.
Angelique Pothuizen et al.
Vanadium-dependent haloperoxidases (VHPOs) are attractive biocatalysts for halofunctionalisation chemistry, but their routine use is frequently constrained by poor soluble recombinant expression. Here, we explore protein fusion as a constru...
María José Gómara,Ignacio Pérez-Pomeda,Ramon Pons et al.
María José Gómara et al.
Broad spectrum anti-inflammatory peptide amphiphiles (AIF-PAs) have been developed as an innovative strategy for the treatment of chronic mucosal inflammation and the prevention of HIV transmission. Novel lipopeptides based on two linear se...
Biocatalytic Potential of a Mycobacterial Aminoacylase for Synthesis of N-Acyl-L-Amino Acids in Aqueous Media [0.03%]
用于水相中N-酰基氨基酸合成的芽孢杆菌氨基酰化酶的生物催化潜力研究
Jessika Wirges,Gerrit Haeger,Laureline Gennesseaux et al.
Jessika Wirges et al.
In this study, we present an investigation of the recently identified aminoacylase MsAA for the synthesis of N-acyl-L-amino acids, focusing on N-lauroyl-L-methionine. We found optimal reaction conditions at pH 8.0 and a temperature of 40-45...
Laccase-Catalyzed Dimerization of Honokiol and Magnolol for Multitarget Metabolic Enzyme Inhibitors [0.03%]
漆酶催化汉防己甲素和Magnolol二聚体化制备多靶点多胺代谢酶抑制剂
Claudia Sciacca,Nunzio Cardullo,Luana Pulvirenti et al.
Claudia Sciacca et al.
Laccases are biocatalysts with a high potential for developing green procedures to achieve new compounds. This study employed laccases in buffer solution to synthesize dimeric neolignans from honokiol and magnolol. Four laccases (LTV, POXA1...
70 Years of Polyphosphate Kinase: Expanding Functions and Emerging Design Considerations [0.03%]
多聚焦磷酸酶七十年：功能拓展和设计思考涌现
Zong-Lin Li
Zong-Lin Li
Polyphosphate kinases (PPKs) have long served as practical tools for ATP regeneration. Recent reports demonstrating broader nucleotide specificity have renewed interest in this enzyme family and expanded its perceived functional scope. At t...
Mechanistic Basis of Cholesterol Binding and Transfer in NPC2: Insights From Molecular Dynamics Simulations [0.03%]
胆固醇结合和转运在NPC2中的机制基础：来自分子动力学模拟的见解
Smit Patel,Nadia Elghobashi-Meinhardt
Smit Patel
The Niemann-Pick type C (NPC) disease is characterized by impaired function of the lysosomal proteins NPC1 or NPC2. The soluble NPC2 protein is believed to transport cholesterol to the larger, membrane-bound NPC1, thereby maintaining choles...