Environmental Impact of Synthetic Cell Technology: Review of Life Cycle Assessment Data for Feedstocks and Production Methods [0.03%]
合成细胞技术的环境影响：原料和生产方法生命周期评价数据回顾
Christina Hopf,Mehdi Ravandeh,Jan Steinkühler
Christina Hopf
Bottom-up synthetic cells are frequently framed as enabling technologies for a future green bioeconomy, yet their environmental impacts remain poorly quantified. Here, we review and synthesize available life cycle assessment (LCA) data for ...
UGT707B2 From Iris tectorum Maxim.: A Promiscuous Glycosyltransferase for the Biosynthesis of Structurally Diverse Flavonoid Glycosides [0.03%]
黄菖蒲UGT707B2: 能合成多种结构类黄酮糖苷的多功能糖基转移酶
Lin Zhang,Jing Li,Qian Zhang et al.
Lin Zhang et al.
Iris tectorum Maxim. (I. tectorum) is a medicinal plant in China, known for its efficacy in treating upper respiratory diseases. It contains an abundance of (iso)flavonoid glycosides, which exhibit extensive pharmacological activities. Due ...
Maxim van Delft,Alejandro Gran-Scheuch,Ulf Hanefeld et al.
Maxim van Delft et al.
In the search for more efficient chemical processes to valorize renewable feedstocks, oleate hydratases (Ohys) represent promising biocatalysts. These enzymes can convert cis-Δ9 fatty acids like oleic acid into their hydroxy fatty acid cou...
Recent Advances in Fluorescent Probes for Imaging Enzyme Activity in NETosis [0.03%]
用于NETosis中成像酶活性的荧光探针最新进展
Enebie Ramos Cáceres,Marijn J Hollander,Kimberly M Bonger
Enebie Ramos Cáceres
Neutrophil extracellular traps (NETs) are web-like structures of DNA released by neutrophils during a specialized form of cell death called NETosis. Originally described as a mechanism of pathogen neutralization, NETs have since been implic...
Generation of DAR1 Glycosite-Specific Antibody-Drug Conjugates Using DisacLink Technology [0.03%]
利用DisacLink技术生成DAR1糖基位点特异性抗体药物偶联物
Yaqi Shi,Yan Zhao,Zhi Liu et al.
Yaqi Shi et al.
The drug-to-antibody ratio (DAR) represents a critical quality attribute for antibody-drug conjugates (ADCs). Conventional site-specific conjugation strategies typically generate ADCs with defined DAR values ranging from 2 to 8. However, fo...
Light-Activated Communication Between Synthetic and Living Cells via Photoresponsive Membranes [0.03%]
用于合成细胞和活细胞之间光激活通讯的光响应膜
Roberto Barbaro,Paola Albanese,Matilde Colella et al.
Roberto Barbaro et al.
Synthetic cells (SCs) are programmable biomimetic systems that reproduce selected cellular functions while offering high structural and functional controllability. A particularly attractive feature of SCs is their ability to respond to exte...
Label-Free Monitoring of Cancer-Associated Fibroblast Activation Using NAD(P)H Fluorescence Lifetime Imaging [0.03%]
基于NAD(P)H荧光寿命成像的癌相关成纤维细胞活化无标记检测技术研究
Nina Anseeuw,Carmen Escalona-Noguero,Giel Vankevelaer et al.
Nina Anseeuw et al.
Cancer-associated fibroblasts (CAFs) are key regulators of tumor progression, yet their activation state is commonly assessed using static, endpoint assays that do not allow dynamic analysis in living cells. Although CAF activation is accom...
Azurins With Tunable Reduction Potentials as Redox Mediators for Extracellular Electron Transfer [0.03%]
具有可调还原电位的细胞色素azurin作为细胞外电子传递的氧化还原介体
Alexis J Holwerda,Jing-Xiang Wang,Yang Gao et al.
Alexis J Holwerda et al.
Electroactive microbes, such as Shewanella oneidensis MR-1, have been harnessed for a variety of bioelectrochemical and catalytic applications. Efforts to improve the electrochemical performance of these bacteria have included the use or bi...
S-Adenosyl-d-Methionine as a Non-Physiological Substrate for a Wide Range of SAM-Dependent Enzymes [0.03%]
S-腺苷甲硫氨酸作为多种SAM依赖型酶的非生理底物
Philipp Germer,Lukas Gericke,Lars-Hendrik Koeppl et al.
Philipp Germer et al.
The ability of SAM-dependent enzymes to accept S-adenosyl-d-methionine [d-SAM, (SS,RCα)-SAM] instead of the native cofactor S-adenosyl-l-methionine [l-SAM, (SS,SCα)-SAM] remains largely unexplored. Challenging the stereochemical preferenc...
Joseph M Heili,Paul H Mroch,Tanner G Hoog et al.
Joseph M Heili et al.
Light-up RNA aptamers that bind nonfluorescent dyes and conditionally activate their fluorescence are powerful tools for RNA imaging and biomolecular sensing. A barrier to widespread adoption of this technology is a reliance on bespoke dyes...