Human cathepsin H: deletion of the mini-chain switches substrate specificity from aminopeptidase to endopeptidase [0.03%]
Johannes Dodt,Jörg Reichwein
Johannes Dodt
The mini-chain of human cathepsin H has been identified as the major structural element determining the protease's substrate specificity. A genetically engineered mutant of human cathepsin H lacking the mini-chain, des[Glu(-18)-Thr(-11)]-ca...
Bioactivatable, membrane-permeant analogs of cyclic nucleotides as biological tools for growth control of C6 glioma cells [0.03%]
Martin Bartsch,Michaela Zorn-Kruppa,Nicole Kühl et al.
Martin Bartsch et al.
In the present study, the cAMP analogs 8-bromo-cAMP (8-Br-cAMP), N6-2'O-dibutyryl-cAMP (DBcAMP) and 8-para-chlorophenylthio-cAMP (8-CPT-cAMP), as well as the corresponding cAMP-acetoxymethyl (AM)-ester-prodrugs were tested in a HPLC study f...
Kinin-B1 receptors in ischaemia-induced pancreatitis: functional importance and cellular localisation [0.03%]
Joachim F Kuebler,Elisabeth Schremmer-Danninger,Kanti D Bhoola et al.
Joachim F Kuebler et al.
In this study we compare the role of kinin-B1 and B2 receptors during ischaemia/reperfusion of rat pancreas. Our investigations were prompted by the observation that infusion of a kinin-B2 receptor antagonist produced significant improvemen...
Comparative Study
Biological chemistry. 2003 Sep;384(9):1311-9. DOI:10.1515/BC.2003.147 2003
Heike Budde,Leopold Flohé,Birgit Hofmann et al.
Heike Budde et al.
Tryparedoxin peroxidases (TXNPx) catalyze hydroperoxide reduction by tryparedoxin (TXN) by an enzyme substitution mechanism presumed to involve three catalytic intermediates: (i) a transient oxidation state having C52 oxidized to a sulfenic...
Guohua Jiang,Qun Wei
Guohua Jiang
Calcineurin (CN), a Ca2+/calmodulin-dependent protein phosphatase, plays a critical role in T-cell activation by regulating the activity of NF-AT. CN is a heterodimer consisting of a catalytic subunit (CNA) and a Ca2+-binding regulatory sub...
Phosphatidylinositol-3,5-Bisphosphate is a potent and selective inhibitor of acid sphingomyelinase [0.03%]
Melanie Kölzer,Christoph Arenz,Klaus Ferlinz et al.
Melanie Kölzer et al.
Acid sphingomyelinase (A-SMase, EC 3.1.4.12) catalyzes the lysosomal degradation of sphingomyelin to phosphorylcholine and ceramide. Inherited deficiencies of acid sphingomyelinase activity result in various clinical forms of Niemann-Pick d...
A nucleosome-free dG-dC-rich sequence element promotes constitutive transcription of the essential yeast RIO1 gene [0.03%]
Michaela Angermayr,Kerstin Schwerdffeger,Wolfhard Bandlow
Michaela Angermayr
RIO1 is an essential gene that encodes a protein serine kinase and is transcribed constitutively at a very low level. Transcriptional activation of RIO1 dispenses with a canonical TATA box as well as with classical transactivators or specif...
Gregor Hutter,Frank L Heppner,Adriano Aguzzi
Gregor Hutter
Prion diseases are characterized by the deposition of PrP(Sc), an abnormal form of the cellular prion protein PrP(C), which is encoded by the Prnp gene. PrP(C) is highly expressed on neurons and its function is unknown. Recombinant PrP(C) w...
The making of a professional secretory cell: architectural and functional changes in the ER during B lymphocyte plasma cell differentiation [0.03%]
Luigina Tagliavacca,Tiziana Anelli,Claudio Fagioli et al.
Luigina Tagliavacca et al.
B lymphocytes are small cells that express antigen receptors and secrete little if any IgM. Upon encounter with antigen, they differentiate into short-lived plasma cells, which secrete large amounts of polymeric IgM. Plasma cell differentia...
Marcus A Koch,Rolf Breinbauer,Herbert Waldmann
Marcus A Koch
Proteins are modularly built from a limited set of approximately 1000 structural domains. The evolutionary relationship within a domain family suggests that the knowledge about a common fold structure can be exploited for the design of smal...