Solid-state fermentation of corn gluten meal with Aspergillus oryzae 6002 enhances amino acid and dipeptide accumulation while retaining high protein content [0.03%]
固态发酵提高玉米蛋白粉中氨基酸和二肽积累的同时保持其高蛋白含量
Tadashi Hara,Jirayu Jitpakdee,Natsuki Fukano et al.
Tadashi Hara et al.
Corn gluten meal (CGM) is a protein-rich by-product of corn starch production that is primarily used as animal feed. In this study, we aimed to enhance the value of CGM while retaining its high-protein characteristics through solid-state fe...
Metabolic bottleneck at TCA cycle entry point and competition with trehalose production hinder growth of methylotrophic yeasts during methanol assimilation [0.03%]
甲烷营养型酵母在甲醇同化过程中三羧酸循环入口处的代谢瓶颈及其与海藻糖产生的竞争限制了其生长
Ayumu Kamiyama,Taisuke Seike,Nobuyuki Okahashi et al.
Ayumu Kamiyama et al.
Methylotrophic yeasts are promising hosts for bioproduction from methanol, but their growth is significantly reduced in the presence of methanol. In this study, we investigated metabolic bottlenecks in Komagataella phaffii, Ogataea polymorp...
Enzymatic degradation of γ-oryzanol and dibutyl phthalate by Aspergillus oryzae lipase [0.03%]
米曲霉脂肪酶对γ-稻谷醇和己二酸二丁酯的降解作用研究
Haruna Sato,Kyotaro Ichikawa,Yoshihito Shiono et al.
Haruna Sato et al.
Phthalate esters, widely used as plasticizers in plastic manufacturing, are known for their endocrine-disrupting effects. γ-Oryzanol derivatives, functional lipids composed of sterol and ferulic acid esters, possess diverse biological acti...
Constitutive and inducer-free production of xanthine oxidase in a Pseudomonas putida cell factory [0.03%]
铜绿假单胞菌细胞工厂中异源过表达黄嘌呤氧化酶
Xiaoyan Guo,Xueying Guo,Yuan Gao et al.
Xiaoyan Guo et al.
A robust heterologous expression system was developed in Pseudomonas putida KT2440 for the production of recombinant xanthine oxidase (XOD) from Cellulosimicrobium sp. TH20. To alleviate metabolic burden and enable inducer-free expression, ...
Lipid extraction from oleaginous yeast cells with very high lipid content, low cell density, and fragile cell wall [0.03%]
脂质含量非常高、细胞密度低且细胞壁脆弱的产油酵母细胞的脂质提取方法研究
Ya Wang,Bingyan Zhang,Umutumwa E Principe et al.
Ya Wang et al.
The newly developed oleaginous yeast cells behave high lipid content, low cell density, and fragile cell walls, as well as partial cell disruption. To extract the microbial lipid from these very high lipid containing cells, the regular Folc...
Preferential correction of target genes by 5'-tailed duplexes with an antisense editor strand [0.03%]
具反义编辑链的5'-带尾双链体优先修正靶基因
Taiki Kato,Hidehiko Kawai,Ryotaro Kamitsubo et al.
Taiki Kato et al.
The correction (editing) of mutated genes at the DNA level is expected to cure gene-inherited diseases and cancers. A 5'-tailed duplex (TD) with an approximately 80-base editor strand (E-strand) plus a 35-base assistant strand (A-strand) wa...
Outer root sheath cells secrete oxytocin for hair growth promotion and stem cell proliferation [0.03%]
外根鞘细胞分泌缩宫素促进毛发增长和干细胞增殖
Tatsuto Kageyama,Ting Sun,Lei Yan et al.
Tatsuto Kageyama et al.
Oxytocin is a hormone that is primarily produced in the hypothalamus, as well as in the mammary glands, uterus, intestines, and skin. Generally, this hormone acts on the cells surrounding the tissues to maintain homeostasis. Furthermore, ox...
Comparative transcriptomic profiling of high-yielding Saccharopolyspora pogona mutants generated by consecutive N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis [0.03%]
连续使用N-甲基-N'-硝基-N-硝普胍诱发的高产Saccharopolyspora pogona突变菌株转录图谱分析
Chao Guo,Xinying Li,Jun Li et al.
Chao Guo et al.
Butenyl-spinosyn is a highly effective macrolide insecticide produced by Saccharopolyspora pogona, but its low yield remains a major bottleneck for large-scale industrial application. In this study, a strategy based on consecutive N-methyl-...
Prediction of the current state of beer fermentation indicators using volatile organic compounds from fermentation gas [0.03%]
基于发酵气体挥发性有机物的啤酒发酵指标预测模型研究
Keisuke Yamauchi,Fumika Kimura,Masahiro Furuno et al.
Keisuke Yamauchi et al.
Microbial fermentation is widely used in the production of food, pharmaceuticals, and bioenergy. Proper monitoring of the fermentation process is essential to ensure consistent product quality and yield. Although the indicators of fermentat...
Construction of a high-throughput cell-free system for screening thermostable laccase with redox-responsive hydrogel beads [0.03%]
一种用于筛选具有还原响应型水凝胶微球的耐热漆酶的体外高效筛选系统
Kensei Orita,Kosuke Minamihata,Noriho Kamiya
Kensei Orita
Thermostable enzymes are highly desirable for industrial applications. Conventional thermostability screening typically relies on microplate assays; however, this approach becomes impractical as the library size increases exponentially with...