Microwave pretreatment of glycogen-containing cyanobacteria Synechococcus elongatus UTEX 2973 to enhance enzymatic saccharification and fermentation for bioethanol production [0.03%]
微波预处理含糖原的鱼腥蓝藻集胞藻UTEX 2973以增强其酶解和发酵产乙醇性能
Kazuaki Ninomiya,Tomoko Hashitani
Kazuaki Ninomiya
This study aimed to investigate whether that microwave pretreatment of glycogen-containing Synechococcus elongatus UTEX 2973 could enhance subsequent enzymatic saccharification and ethanol fermentation. The first experiment found that pretr...
Three carbohydrate-binding modules of laminarinase LA-Lam recognize β-1,3-glucan and enhance hydrolytic activity [0.03%]
三个 laminarin酶LA-Lam碳水化合物结合模块可识别β-1,3- glucan并增强其催化活性
Masahiko Okai,Nozomi Ohki,Kengo Nagase et al.
Masahiko Okai et al.
Carbohydrate-binding modules (CBMs) promote enzyme-substrate association, enhancing catalytic activity toward soluble and insoluble substrates. In this study, we characterized the three tandem domains of laminarinase LA-Lam from Pseudoalter...
Cre-loaded integrase-defective lentiviral vectors for targeted cassette exchange in CHO cells [0.03%]
一种在CHO细胞中进行靶向盒交换的Cre载入整合酶缺陷型慢病毒载体
Yoshinori Kawabe,Suguru Imanishi,Takuya Shimomura et al.
Yoshinori Kawabe et al.
Genome-modifying enzymes, such as recombinases and CRISPR-associated nucleases, enable targeted gene insertion when delivered transiently to minimize off-target effects. Precise genome engineering requires controlled enzyme activity, as wel...
Structure- and phylogeny-guided redirection of substrate specificity in diaminobutyric acid racemase PddB toward ornithine and lysine [0.03%]
基于结构和系统发育的天冬氨酸 racemase(PddB)底物专一性重编程以作用于鸟氨酸和赖氨酸
Kazuya Yamanaka,Yudai Okuno,Tadao Oikawa
Kazuya Yamanaka
Substrate specificity of amino acid racemases is a key determinant of their biological function, yet the structural principles governing this specificity, particularly with respect to substrate side-chain structure, remain poorly understood...
Identification of key enzymes involved in 1,2,4-trihydroxybenzene metabolism in the hyper lignin-degrading fungus Phanerochaete sordida YK-624 [0.03%]
高木质素降解真菌Phanerochaete sordida YK-624中参与1,2,4-三羟基苯甲醚代谢的关键酶鉴定
Akiko Ono,Wonhi Park,Ru Yin et al.
Akiko Ono et al.
Lignin is a complex aromatic polymer and a major component of lignocellulosic biomass, whose sustainable utilization is critical for the development of a low-carbon society. The white-rot fungus Phanerochaete sordida YK-624 exhibits high li...
Adaptive laboratory evolution of Methylorubrum extorquens for lanthanide-dependent robust growth under high concentrations of methanol [0.03%]
甲醇高浓度下适应性实验室进化工程假单胞菌生产稀土元素依赖的生长体系
Tsuneyoshi Chiba,Toshiaki Fukui,Izumi Orita
Tsuneyoshi Chiba
Methylorubrum extorquens AM1 has been extensively studied as a platform for biomanufacturing using a methanol feedstock. Given that lanthanide (Ln) ions are recognized as important metals related to methylotrophy of methylotrophic bacteria,...
Cas9-PALB2 fusion protein enhances CRISPR/Cas9 mediated gene knock-in efficiency [0.03%]
一种融合PALB2的Cas9蛋白可提高CRISPR-Cas9介导的基因敲入效率
Dehao Meng,Yanfen Zhang,Shuang Zou et al.
Dehao Meng et al.
Over the past decade, CRISPR-based technologies have revolutionized our capacity to manipulate genomes, thereby reshaping the landscape of functional genomics research. Among the CRISPR toolkit, CRISPR/Cas9-mediated homology-directed repair...
Gate-to-gate life cycle assessment of sophorolipid production with Starmerella bombicola: Impact of cultivation conditions and scale-up on carbon footprint [0.03%]
星形假丝酵母生产皂皮脂门对门生命周期评价：培养条件和放大的碳足迹影响分析
Pijar Religia,Chih-Chan Wu,Hidemasa Oda et al.
Pijar Religia et al.
Sophorolipid, a biosurfactant which can be fermentatively produced by the yeast Starmerella bombicola using sugars and lipids as carbon sources, exhibits several advantageous properties over synthetic detergents, such as biodegradability an...
Elucidation of a novel fungal biotransformation pathway of ibuprofen by Phanerochaete sordida YK-624 under non-ligninolytic conditions [0.03%]
卡那霉素抗性标记的安全回收方法研究进展
Wonhi Park,Ru Yin,Akiko Ono et al.
Wonhi Park et al.
Ibuprofen, a widely used non-steroidal anti-inflammatory drug (NSAID), has been detected in the environment and is known to contaminate waterbodies. Here, we investigated the biotransformation of ibuprofen using the hyper-lignin-degrading f...
Genetic interaction between the septation initiation network component SepM and the cell wall integrity transcription factor RlmA regulates cellobiose-responsive CAZyme expression in Aspergillus aculeatus [0.03%]
Aspergillus aculeatus中的分离启动网络成分SepM与细胞壁完整性转录因子RlmA的遗传相互作用调控鼠李糖枝孢霉中的半乳糖苷酶表达
Yui Shiga,Kazumi Sawada,Saki Kikuya et al.
Yui Shiga et al.
We previously reported that SepL and SepM, components of the septation initiation network (SIN) complex, are involved in the activation of the ManR-dependent cellobiose-responsive expression in the cellulase-producing fungus Aspergillus acu...