Enhanced tRNA array method version 2 for simultaneous in vitro synthesis of 21 tRNAs [0.03%]
改进的tRNA阵列法版本2：体外同时合成21种tRNA
Ryota Miyachi,Anna Irie,Norikazu Ichihashi
Ryota Miyachi
Transfer RNAs (tRNAs) play an essential role in translation, and their simultaneous in vitro synthesis remains a key challenge in bottom-up synthetic biology. We previously developed the tRNA array method that enables the simultaneous in vi...
Employing synthetic biology to modulate metabolic flux: Current strategies and applications [0.03%]
利用合成生物学调控代谢通量：当前策略与应用
Divya Ojha,Ram Kulkarni,Ambadas B Rode
Divya Ojha
The world's increasing demand for petrochemical, pharmaceutical, and nutraceutical products necessitates the development of new strategies for producing these high-value chemicals. The depletion of the natural fossil reserves and environmen...
Cell-free screening of peptide tags for droplet-like assembly formation of glucose dehydrogenase [0.03%]
无细胞筛选葡萄糖脱氢酶类droplet-like组装所需的肽标签
Sayoko Ito-Harashima,Koharu Alicia Senda,Ako Kagawa et al.
Sayoko Ito-Harashima et al.
Glucose dehydrogenases (GDHs) are oxidoreductases that catalyze the oxidation of d-glucose to glucono-δ-lactone and are widely used in industrial applications such as biosensors, bioelectrodes, and biocatalytic cofactor regeneration. In pa...
Efficient hybridoma screening using extracellular vesicles for conformation-specific antibodies to transmembrane proteins [0.03%]
用于膜蛋白构象特异性抗体筛选的细胞外囊泡高效杂交瘤技术
Rina Sakamaki,Takao Matsuba,Yasuyuki Kurihara
Rina Sakamaki
Here, we report a simple and rapid extracellular vesicle (EV)-based membrane-type immunoglobulin-directed hybridoma screening (MIHS) platform that enables efficient isolation of monoclonal antibodies (mAbs) recognizing three-dimensional con...
Role of the CYR1G2066A mutation in ethanol tolerance of sake yeast Kyokai no. 11 [0.03%]
CYR1G2066A突变在清酒酿酒酵母Kyokai no. 11耐乙醇性中的作用
Kazuya Morinaka,Yoshihiro Isida,Mamoru Watanabe et al.
Kazuya Morinaka et al.
Sake yeast Kyokai no. 11 (K11) is an ethanol-tolerant mutant of Kyokai no. 7 (K7) and produces a higher ethanol concentration in the sake mash than K7. A previous study revealed that stress-induced genes under the control of STRE elements w...
Biofilm-microenvironment responsive hybrid micelle based on novel betaine derivative for enhanced delivery of honokiol against stubborn bacterial biofilms [0.03%]
一种新型甜菜碱衍生物的杂化胶束，响应生物膜微环境并用于高效递送Honokiol对抗顽固细菌生物膜
Lian Chen,Awn Abbas,Nanxin Li et al.
Lian Chen et al.
The development of conventional antibiotics is being severely challenged by the rise of bacterial resistance and the obstacle of biofilm-associated infections. Given their enhanced permeability, nano micelles have emerged as a widely utiliz...
Biocatalysis in microaqueous systems (solvent-based and solvent-free conditions): Historical perspectives and recent advances [0.03%]
微含水体系(溶剂型和非溶剂型)中的生物催化：历史观点与近期进展
Tsuneo Yamane
Tsuneo Yamane
A microaqueous system is generally defined as a system containing a small amount of water in the bulk of a medium, yet playing a pivotal role that exists between two extreme systems: aqueous systems and nonaqueous (or anhydrous, or non-aque...
Chemoenzymatic method for site-selective fluorophore conjugation of a native IgG Fab fragment [0.03%]
用于天然IgG Fab片段位点特异性荧光标记的化学酶法
Eva Agustriana,Koki Murozono,Riko Nishioka et al.
Eva Agustriana et al.
Antibody-derived bioconjugates have emerged as practical biomolecules for applications in diagnostics and therapeutics. A key challenge is achieving site-selective bioconjugation to preserve the native function of the labeled biomolecule. M...
Methanol valorization into glycerol by engineering Bacillus methanolicus with thermostable enzymes and a tunable promoter library [0.03%]
利用耐热酶和可调启动子文库工程化改造产甲醇芽孢杆菌将甲醇转化为甘油
Bo Xin,Miao Li,Jingyu Liu et al.
Bo Xin et al.
Glycerol serves as a versatile C3 platform chemical with broad applications spanning pharmaceuticals, personal care, food, cosmetics, and as a renewable feedstock for value-added chemicals, fuels, and materials. Methanol, a promising substr...
Solid-state fermentation of corn gluten meal with Aspergillus oryzae 6002 enhances amino acid and dipeptide accumulation while retaining high protein content [0.03%]
固态发酵提高玉米蛋白粉中氨基酸和二肽积累的同时保持其高蛋白含量
Tadashi Hara,Jirayu Jitpakdee,Natsuki Fukano et al.
Tadashi Hara et al.
Corn gluten meal (CGM) is a protein-rich by-product of corn starch production that is primarily used as animal feed. In this study, we aimed to enhance the value of CGM while retaining its high-protein characteristics through solid-state fe...