Innervation of nociceptor neurons in the spleen promotes germinal center responses and humoral immunity [0.03%]
脾脏神经免疫相互作用调控体液免疫响应
Min Wu,Guangping Song,Jianing Li et al.
Min Wu et al.
Peripheral sensory neurons widely innervate various tissues to continuously monitor and respond to environmental stimuli. Whether peripheral sensory neurons innervate the spleen and modulate splenic immune response remains poorly defined. H...
Ligand binding initiates single-molecule integrin conformational activation [0.03%]
配体结合引发单分子整合素构象激活
Jing Li,Myung Hyun Jo,Jiabin Yan et al.
Jing Li et al.
Integrins link the extracellular environment to the actin cytoskeleton in cell migration and adhesiveness. Rapid coordination between events outside and inside the cell is essential. Single-molecule fluorescence dynamics show that ligand bi...
Massively parallel in vivo Perturb-seq reveals cell-type-specific transcriptional networks in cortical development [0.03%]
大规模并行在体扰动序技术揭示了皮层发育中的细胞类型特异性转录网络
Xinhe Zheng,Boli Wu,Yuejia Liu et al.
Xinhe Zheng et al.
Leveraging AAVs' versatile tropism and labeling capacity, we expanded the scale of in vivo CRISPR screening with single-cell transcriptomic phenotyping across embryonic to adult brains and peripheral nervous systems. Through extensive tests...
Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans [0.03%]
疫苗诱导人类异种HIV-1中和抗体B细胞谱系
Wilton B Williams,S Munir Alam,Gilad Ofek et al.
Wilton B Williams et al.
A critical roadblock to HIV vaccine development is the inability to induce B cell lineages of broadly neutralizing antibodies (bnAbs) in humans. In people living with HIV-1, bnAbs take years to develop. The HVTN 133 clinical trial studied a...
Rebecca A Green,Renat N Khaliullin,Zhiling Zhao et al.
Rebecca A Green et al.
Systematic functional profiling of the gene set that directs embryonic development is an important challenge. To tackle this challenge, we used 4D imaging of C. elegans embryogenesis to capture the effects of 500 gene knockdowns and develop...
Philip Francis Thomsen,Mads Reinholdt Jensen,Eva Egelyng Sigsgaard
Philip Francis Thomsen
Environmental DNA (eDNA) has opened promising avenues for establishing standardized, cost-efficient monitoring of biodiversity. However, comprehensive and systematic implementation is urgently needed to address the current biodiversity cris...
ARID1A suppresses R-loop-mediated STING-type I interferon pathway activation of anti-tumor immunity [0.03%]
ARID1A通过抑制R-loop介导的STING型I干扰素通路活化抗肿瘤免疫反应
Matthew B Maxwell,Marianne S Hom-Tedla,Jawoon Yi et al.
Matthew B Maxwell et al.
Clinical trials have identified ARID1A mutations as enriched among patients who respond favorably to immune checkpoint blockade (ICB) in several solid tumor types independent of microsatellite instability. We show that ARID1A loss in murine...
An age-progressive platelet differentiation path from hematopoietic stem cells causes exacerbated thrombosis [0.03%]
造血干细胞到血小板分化进程中年龄渐进式的血小板生成加剧了血栓形成
Donna M Poscablo,Atesh K Worthington,Stephanie Smith-Berdan et al.
Donna M Poscablo et al.
Platelet dysregulation is drastically increased with advanced age and contributes to making cardiovascular disorders the leading cause of death of elderly humans. Here, we reveal a direct differentiation pathway from hematopoietic stem cell...
Sergi Junyent,Maciej Meglicki,Roman Vetter et al.
Sergi Junyent et al.
Retrospective lineage reconstruction of humans predicts that dramatic clonal imbalances in the body can be traced to the 2-cell stage embryo. However, whether and how such clonal asymmetries arise in the embryo is unclear. Here, we performe...
Osr2 functions as a biomechanical checkpoint to aggravate CD8+ T cell exhaustion in tumor [0.03%]
Osr2充当一种生物力学检查点以加剧肿瘤中CD8⁺T细胞的衰竭
Jinjia Zhang,Junhong Li,Yongqiang Hou et al.
Jinjia Zhang et al.
Alterations in extracellular matrix (ECM) architecture and stiffness represent hallmarks of cancer. Whether the biomechanical property of ECM impacts the functionality of tumor-reactive CD8+ T cells remains largely unknown. Here, we reveal ...