Heterologous survey of 130 DNA transposons in human cells highlights their functional divergence and expands the genome engineering toolbox [0.03%]
异源调查人类细胞中的130种DNA转座子突显其功能分歧并扩大基因组工程工具箱
Tongtong Zhang,Shengjun Tan,Na Tang et al.
Tongtong Zhang et al.
Experimental studies on DNA transposable elements (TEs) have been limited in scale, leading to a lack of understanding of the factors influencing transposition activity, evolutionary dynamics, and application potential as genome engineering...
Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss [0.03%]
DNMT3A突变驱动的克隆性血细胞生成促进炎性骨丢失
Hui Wang,Kimon Divaris,Bohu Pan et al.
Hui Wang et al.
Clonal hematopoiesis of indeterminate potential (CHIP) arises from aging-associated acquired mutations in hematopoietic progenitors, which display clonal expansion and produce phenotypically altered leukocytes. We associated CHIP-DNMT3A mut...
DNA-based ForceChrono probes for deciphering single-molecule force dynamics in living cells [0.03%]
基于DNA的ForceChrono探针用于解析活细胞中单分子力的动力学特征
Yuru Hu,Hongyun Li,Chen Zhang et al.
Yuru Hu et al.
Understanding cellular force transmission dynamics is crucial in mechanobiology. We developed the DNA-based ForceChrono probe to measure force magnitude, duration, and loading rates at the single-molecule level within living cells. The Forc...
POT1 recruits and regulates CST-Polα/primase at human telomeres [0.03%]
端粒结合蛋白POT1通过招募和调控CST- Polα/引物酶复合体来参与人类端粒维持
Sarah W Cai,Hiroyuki Takai,Arthur J Zaug et al.
Sarah W Cai et al.
Telomere maintenance requires the extension of the G-rich telomeric repeat strand by telomerase and the fill-in synthesis of the C-rich strand by Polα/primase. At telomeres, Polα/primase is bound to Ctc1/Stn1/Ten1 (CST), a single-stranded...
De novo and salvage purine synthesis pathways across tissues and tumors [0.03%]
从头和补救性嘌呤合成途径在组织和肿瘤中的作用
Diem H Tran,Dohun Kim,Rushendhiran Kesavan et al.
Diem H Tran et al.
Purine nucleotides are vital for RNA and DNA synthesis, signaling, metabolism, and energy homeostasis. To synthesize purines, cells use two principal routes: the de novo and salvage pathways. Traditionally, it is believed that proliferating...
A pseudoautosomal glycosylation disorder prompts the revision of dolichol biosynthesis [0.03%]
一种假常染色体糖基化障碍促使对多胺生物合成的修订
Matthew P Wilson,Takfarinas Kentache,Charlotte R Althoff et al.
Matthew P Wilson et al.
Dolichol is a lipid critical for N-glycosylation as a carrier for activated sugars and nascent oligosaccharides. It is commonly thought to be directly produced from polyprenol by the enzyme SRD5A3. Instead, we found that dolichol synthesis ...
Taehong Yang,Daniel W Bayless,Yichao Wei et al.
Taehong Yang et al.
Published Erratum
Cell. 2024 Jul 11;187(14):3782. DOI:10.1016/j.cell.2024.05.046 2024
RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly [0.03%]
RNA质量控制因子能够聚集Clr4/SUV39H并触发组成型异染色质的形成
Jasbeer S Khanduja,Richard I Joh,Monica M Perez et al.
Jasbeer S Khanduja et al.
In eukaryotes, the Suv39 family of proteins tri-methylate lysine 9 of histone H3 (H3K9me) to form constitutive heterochromatin. However, how Suv39 proteins are nucleated at heterochromatin is not fully described. In the fission yeast, curre...
A functional cellular framework for sex and estrous cycle-dependent gene expression and behavior [0.03%]
与性别和发情周期依赖的基因表达和行为的功能性细胞框架
Joseph R Knoedler,Sayaka Inoue,Daniel W Bayless et al.
Joseph R Knoedler et al.
Published Erratum
Cell. 2024 Jul 11;187(14):3781. DOI:10.1016/j.cell.2024.05.045 2024
Fangyu Liu,Anat Levit Kaplan,Jesper Levring et al.
Fangyu Liu et al.
The cystic fibrosis transmembrane conductance regulator (CFTR) is a crucial ion channel whose loss of function leads to cystic fibrosis, whereas its hyperactivation leads to secretory diarrhea. Small molecules that improve CFTR folding (cor...