Integrative spatial analysis reveals a multi-layered organization of glioblastoma [0.03%]
整合型空间分析揭示了胶质母细胞瘤的多层次组织结构
Alissa C Greenwald,Noam Galili Darnell,Rouven Hoefflin et al.
Alissa C Greenwald et al.
Glioma contains malignant cells in diverse states. Here, we combine spatial transcriptomics, spatial proteomics, and computational approaches to define glioma cellular states and uncover their organization. We find three prominent modes of ...
Evasion of NKG2D-mediated cytotoxic immunity by sarbecoviruses [0.03%]
sarbecovirus逃避NKG2D介导的细胞毒免疫作用
Jordan A Hartmann,Marcella R Cardoso,Maria Cecilia Ramiro Talarico et al.
Jordan A Hartmann et al.
SARS-CoV-2 and other sarbecoviruses continue to threaten humanity, highlighting the need to characterize common mechanisms of viral immune evasion for pandemic preparedness. Cytotoxic lymphocytes are vital for antiviral immunity and express...
Dual-role transcription factors stabilize intermediate expression levels [0.03%]
行使双重职能的转录因子可稳定中间表达水平
Jinnan He,Xiangru Huo,Gaofeng Pei et al.
Jinnan He et al.
Precise control of gene expression levels is essential for normal cell functions, yet how they are defined and tightly maintained, particularly at intermediate levels, remains elusive. Here, using a series of newly developed sequencing, ima...
For Cell's 50th anniversary Focus on Immunology, scientific editor Cheri Sirois asked science communicator Liz Neeley, founding partner of Liminal and cofounder of Solving for Science, to discuss strategies for tackling technical complexity...
Global, site-resolved analysis of ubiquitylation occupancy and turnover rate reveals systems properties [0.03%]
全局位点解析泛素化占有率和周转率的分析揭示了系统属性
Gabriela Prus,Shankha Satpathy,Brian T Weinert et al.
Gabriela Prus et al.
Ubiquitylation regulates most proteins and biological processes in a eukaryotic cell. However, the site-specific occupancy (stoichiometry) and turnover rate of ubiquitylation have not been quantified. Here we present an integrated picture o...
NINJ1 mediates plasma membrane rupture by cutting and releasing membrane disks [0.03%]
NINJ1通过切割并释放膜盘介导质膜破裂
Liron David,Jazlyn P Borges,L Robert Hollingsworth et al.
Liron David et al.
The membrane protein NINJ1 mediates plasma membrane rupture in pyroptosis and other lytic cell death pathways. Here, we report the cryo-EM structure of a NINJ1 oligomer segmented from NINJ1 rings. Each NINJ1 subunit comprises amphipathic (...
RNA genome packaging and capsid assembly of bluetongue virus visualized in host cells [0.03%]
在宿主细胞中可视化蓝舌病毒的RNA基因组包装和衣壳装配过程
Xian Xia,Po-Yu Sung,Michael W Martynowycz et al.
Xian Xia et al.
Unlike those of double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and ssRNA viruses, the mechanism of genome packaging of dsRNA viruses is poorly understood. Here, we combined the techniques of high-resolution cryoelectron microscop...
ITPRIPL1 binds CD3ε to impede T cell activation and enable tumor immune evasion [0.03%]
ITPRIPL1通过结合CD3ε抑制T细胞活化并促进肿瘤免疫逃逸
Shouyan Deng,Yibo Zhang,Huanbin Wang et al.
Shouyan Deng et al.
Cancer immunotherapy has transformed treatment possibilities, but its effectiveness differs significantly among patients, indicating the presence of alternative pathways for immune evasion. Here, we show that ITPRIPL1 functions as an inhibi...
Direct observation of a condensate effect on super-enhancer controlled gene bursting [0.03%]
超增强子调控基因爆发现象的冷凝物效应的直接观察
Manyu Du,Simon Hendrik Stitzinger,Jan-Hendrik Spille et al.
Manyu Du et al.
Published Erratum
Cell. 2024 May 9;187(10):2595-2598. DOI:10.1016/j.cell.2024.04.001 2024
Chromatin context-dependent regulation and epigenetic manipulation of prime editing [0.03%]
染色质环境依赖性调控和表观遗传修饰引导的prime编辑技术及其应用
Xiaoyi Li,Wei Chen,Beth K Martin et al.
Xiaoyi Li et al.
We set out to exhaustively characterize the impact of the cis-chromatin environment on prime editing, a precise genome engineering tool. Using a highly sensitive method for mapping the genomic locations of randomly integrated reporters, we ...