Amplification editing enables efficient and precise duplication of DNA from short sequence to megabase and chromosomal scale [0.03%]
扩增编辑可在从短片段到兆碱基和染色体规模的任意DNA中实现高效精准复制
Ruiwen Zhang,Zhou He,Yajing Shi et al.
Ruiwen Zhang et al.
Duplication is a foundation of molecular evolution and a driver of genomic and complex diseases. Here, we develop a genome editing tool named Amplification Editing (AE) that enables programmable DNA duplication with precision at chromosomal...
Multiplexed single-cell characterization of alternative polyadenylation regulators [0.03%]
单细胞转录本切端位点的多重表征及调控机制分析
Madeline H Kowalski,Hans-Hermann Wessels,Johannes Linder et al.
Madeline H Kowalski et al.
Most mammalian genes have multiple polyA sites, representing a substantial source of transcript diversity regulated by the cleavage and polyadenylation (CPA) machinery. To better understand how these proteins govern polyA site choice, we in...
The primitive endoderm supports lineage plasticity to enable regulative development [0.03%]
原始内胚叶支持谱系可塑性以实现调控发育
Madeleine Linneberg-Agerholm,Annika Charlotte Sell,Alba Redó-Riveiro et al.
Madeleine Linneberg-Agerholm et al.
Mammalian blastocyst formation involves the specification of the trophectoderm followed by the differentiation of the inner cell mass into embryonic epiblast and extra-embryonic primitive endoderm (PrE). During this time, the embryo maintai...
Marie Schott,Daniel León-Periñán,Elena Splendiani et al.
Marie Schott et al.
Spatial transcriptomics (ST) methods unlock molecular mechanisms underlying tissue development, homeostasis, or disease. However, there is a need for easy-to-use, high-resolution, cost-efficient, and 3D-scalable methods. Here, we report Ope...
Sara R Savage,Xinpei Yi,Jonathan T Lei et al.
Sara R Savage et al.
Fewer than 200 proteins are targeted by cancer drugs approved by the Food and Drug Administration (FDA). We integrate Clinical Proteomic Tumor Analysis Consortium (CPTAC) proteogenomics data from 1,043 patients across 10 cancer types with a...
Systematic mapping of organism-scale gene-regulatory networks in aging using population asynchrony [0.03%]
利用人群异步性系统化绘制衰老的基因调控网络
Matthias Eder,Olivier M F Martin,Natasha Oswal et al.
Matthias Eder et al.
In aging, physiologic networks decline in function at rates that differ between individuals, producing a wide distribution of lifespan. Though 70% of human lifespan variance remains unexplained by heritable factors, little is known about th...
TERT activation targets DNA methylation and multiple aging hallmarks [0.03%]
端粒酶反转录酶的激活作用于DNA甲基化和多种衰老标志物
Hong Seok Shim,Jonathan Iaconelli,Xiaoying Shang et al.
Hong Seok Shim et al.
Insufficient telomerase activity, stemming from low telomerase reverse transcriptase (TERT) gene transcription, contributes to telomere dysfunction and aging pathologies. Besides its traditional function in telomere synthesis, TERT acts as ...
The evolution of developmental biology through conceptual and technological revolutions [0.03%]
概念和技术变革驱动的发展生物学演进
Prisca Liberali,Alexander F Schier
Prisca Liberali
Developmental biology-the study of the processes by which cells, tissues, and organisms develop and change over time-has entered a new golden age. After the molecular genetics revolution in the 80s and 90s and the diversification of the fie...
Custom scoring based on ecological topology of gut microbiota associated with cancer immunotherapy outcome [0.03%]
基于肠道微生物生态拓扑的免疫治疗响应定制评分系统
Lisa Derosa,Valerio Iebba,Carolina Alves Costa Silva et al.
Lisa Derosa et al.
The gut microbiota influences the clinical responses of cancer patients to immunecheckpoint inhibitors (ICIs). However, there is no consensus definition of detrimental dysbiosis. Based on metagenomics (MG) sequencing of 245 non-small cell l...
FilamentID reveals the composition and function of metabolic enzyme polymers during gametogenesis [0.03%]
丝状体识别在配子发生过程中代谢酶聚合物的组成和功能
Jannik Hugener,Jingwei Xu,Rahel Wettstein et al.
Jannik Hugener et al.
Gamete formation and subsequent offspring development often involve extended phases of suspended cellular development or even dormancy. How cells adapt to recover and resume growth remains poorly understood. Here, we visualized budding yeas...