Method of moments framework for differential expression analysis of single-cell RNA sequencing data [0.03%]
单细胞RNA测序数据的差异表达分析的矩法框架
Min Cheol Kim,Rachel Gate,David S Lee et al.
Min Cheol Kim et al.
Differential expression analysis of single-cell RNA sequencing (scRNA-seq) data is central for characterizing how experimental factors affect the distribution of gene expression. However, distinguishing between biological and technical sour...
Mechanisms of memory-supporting neuronal dynamics in hippocampal area CA3 [0.03%]
海马CA3区记忆相关的神经动力学机制研究
Yiding Li,John J Briguglio,Sandro Romani et al.
Yiding Li et al.
Hippocampal CA3 is central to memory formation and retrieval. Although various network mechanisms have been proposed, direct evidence is lacking. Using intracellular Vm recordings and optogenetic manipulations in behaving mice, we found tha...
A multimodal zebrafish developmental atlas reveals the state-transition dynamics of late-vertebrate pluripotent axial progenitors [0.03%]
斑马鱼多模态发育图谱揭示了后位脊椎动物二元多能轴干细胞的状态转换动力学
Merlin Lange,Alejandro Granados,Shruthi VijayKumar et al.
Merlin Lange et al.
Elucidating organismal developmental processes requires a comprehensive understanding of cellular lineages in the spatial, temporal, and molecular domains. In this study, we introduce Zebrahub, a dynamic atlas of zebrafish embryonic develop...
Clade I mpox virus genomic diversity in the Democratic Republic of the Congo, 2018-2024: Predominance of zoonotic transmission [0.03%]
2018年至2024年刚果民主共和国的I支猴痘病毒基因多样性：人畜共患病传播占主导地位
Eddy Kinganda-Lusamaki,Adrienne Amuri-Aziza,Nicolas Fernandez-Nuñez et al.
Eddy Kinganda-Lusamaki et al.
Recent reports raise concerns on the changing epidemiology of mpox in the Democratic Republic of the Congo (DRC). High-quality genomes were generated for 337 patients from 14/26 provinces to document whether the increase in number of cases ...
Mechanistic study of a low-power bacterial maintenance state using high-throughput electrochemistry [0.03%]
利用高通量电化学研究低能耗细菌维持状态的机制研究
John A Ciemniecki,Chia-Lun Ho,Richard D Horak et al.
John A Ciemniecki et al.
Mechanistic studies of life's lower metabolic limits have been limited due to a paucity of tractable experimental systems. Here, we show that redox-cycling of phenazine-1-carboxamide (PCN) by Pseudomonas aeruginosa supports cellular mainten...
Adaptive multi-epitope targeting and avidity-enhanced nanobody platform for ultrapotent, durable antiviral therapy [0.03%]
一种针对病毒的超长效抗病毒疗法的适应性多表位靶向和亲合力增强型纳米抗体平台
Yufei Xiang,Jialu Xu,Briana L McGovern et al.
Yufei Xiang et al.
Pathogens constantly evolve and can develop mutations that evade host immunity and treatment. Addressing these escape mechanisms requires targeting evolutionarily conserved vulnerabilities, as mutations in these regions often impose fitness...
P-stalk ribosomes act as master regulators of cytokine-mediated processes [0.03%]
P stalk 核糖体作为细胞因子介导过程的主要调节者
Anna Dopler,Ferhat Alkan,Yuval Malka et al.
Anna Dopler et al.
Inflammatory cytokines are pivotal to immune responses. Upon cytokine exposure, cells enter an "alert state" that enhances their visibility to the immune system. Here, we identified an alert-state subpopulation of ribosomes defined by the p...
Potent efficacy of an IgG-specific endoglycosidase against IgG-mediated pathologies [0.03%]
特异靶向IGG的内切糖苷酶在治疗IGG介导性疾病中的潜力疗效
Diego E Sastre,Stylianos Bournazos,Jonathan Du et al.
Diego E Sastre et al.
Endo-β-N-acetylglucosaminidases (ENGases) that specifically hydrolyze the Asn297-linked glycan on immunoglobulin G (IgG) antibodies, the major molecular determinant of fragment crystallizable (Fc) γ receptor (FcγR) binding, are exceeding...
A two-front nutrient supply environment fuels small intestinal physiology through differential regulation of nutrient absorption and host defense [0.03%]
双重供营养环境通过差异调节营养吸收和宿主防御来驱动小肠生理学
Jian Zhang,Ruonan Tian,Jia Liu et al.
Jian Zhang et al.
The small intestine contains a two-front nutrient supply environment created by luminal dietary and microbial metabolites (enteral side) and systemic metabolites from the host (serosal side). Yet, it is unknown how each side contributes dif...
Light-induced targeting enables proteomics on endogenous condensates [0.03%]
光诱导定位技术使内源性凝聚体的蛋白质组学研究成为可能
Choongman Lee,Andrea Quintana,Ida Suppanz et al.
Choongman Lee et al.
Endogenous condensates with transient constituents are notoriously difficult to study with common biological assays like mass spectrometry and other proteomics profiling. Here, we report a method for light-induced targeting of endogenous co...