Transcriptome-scale RNA-targeting CRISPR screens reveal essential lncRNAs in human cells [0.03%]
基于转录组规模RNA导向的CRISPR筛选发现人类细胞中的必需长链非编码RNA
Wen-Wei Liang,Simon Müller,Sydney K Hart et al.
Wen-Wei Liang et al.
Mammalian genomes host a diverse array of RNA that includes protein-coding and noncoding transcripts. However, the functional roles of most long noncoding RNAs (lncRNAs) remain elusive. Using RNA-targeting CRISPR-Cas13 screens, we probed ho...
Jinmin Liu,Amogh Kulkarni,Yong-Qi Gao et al.
Jinmin Liu et al.
Proximity labeling technologies are limited to indexing localized protein residents. Such data-although valuable-cannot inform on small-molecule responsivity of local residents. We here bridge this gap by demonstrating in live C. elegans ho...
Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress [0.03%]
氧化应激下BACH1四聚体结构降解子被两类F盒蛋白识别
Shiyun Cao,Sheena Faye Garcia,Huigang Shi et al.
Shiyun Cao et al.
Ubiquitin-dependent proteolysis regulates diverse cellular functions with high substrate specificity, which hinges on the ability of ubiquitin E3 ligases to decode the targets' degradation signals, i.e., degrons. Here, we show that BACH1, a...
Multimodal targeting chimeras enable integrated immunotherapy leveraging tumor-immune microenvironment [0.03%]
多模态靶向嵌合体能够利用肿瘤免疫微环境进行综合免疫治疗
Feng Lin,Shenyi Yin,Zijian Zhang et al.
Feng Lin et al.
Although immunotherapy has revolutionized cancer treatment, its efficacy is affected by multiple factors, particularly those derived from the complexity and heterogeneity of the tumor-immune microenvironment (TIME). Strategies that simultan...
Qingliang Hu,Huihui Liu,Yajun He et al.
Qingliang Hu et al.
Strigolactones (SLs) are hormones essential for plant development and environmental responses. SL perception requires the formation of the complex composed of an SL receptor DWARF14 (D14), F-box protein D3, and transcriptional repressor D53...
Spatial transcriptomic landscape unveils immunoglobin-associated senescence as a hallmark of aging [0.03%]
空间转录组景观揭示免疫球蛋白相关衰老是衰老的标志事件
Shuai Ma,Zhejun Ji,Bin Zhang et al.
Shuai Ma et al.
To systematically characterize the loss of tissue integrity and organ dysfunction resulting from aging, we produced an in-depth spatial transcriptomic profile of nine tissues in male mice during aging. We showed that senescence-sensitive sp...
Type III interferons induce pyroptosis in gut epithelial cells and impair mucosal repair [0.03%]
III型干扰素诱导肠道上皮细胞发生焦亡并抑制粘膜修复
Kautilya K Jena,Julien Mambu,Daniel Boehmer et al.
Kautilya K Jena et al.
Tissue damage and repair are hallmarks of inflammation. Despite a wealth of information on the mechanisms that govern tissue damage, mechanistic insight into how inflammation affects repair is lacking. Here, we investigated how interferons ...
Selective degradation of multimeric proteins by TRIM21-based molecular glue and PROTAC degraders [0.03%]
基于TRIM21的分子胶和PROTAC降解剂的选择性降解多聚蛋白
Panrui Lu,Yalong Cheng,Lei Xue et al.
Panrui Lu et al.
Targeted protein degradation (TPD) utilizes molecular glues or proteolysis-targeting chimeras (PROTACs) to eliminate disease-causing proteins by promoting their interaction with E3 ubiquitin ligases. Current TPD approaches are limited by re...
Shanghua Gao,Ada Fang,Yepeng Huang et al.
Shanghua Gao et al.
We envision "AI scientists" as systems capable of skeptical learning and reasoning that empower biomedical research through collaborative agents that integrate AI models and biomedical tools with experimental platforms. Rather than taking h...
Ivan N Zheludev,Robert C Edgar,Maria Jose Lopez-Galiano et al.
Ivan N Zheludev et al.
Here, we describe "obelisks," a class of heritable RNA elements sharing several properties: (1) apparently circular RNA ∼1 kb genome assemblies, (2) predicted rod-like genome-wide secondary structures, and (3) open reading frames encoding ...