Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits [0.03%]
同时进行CRISPR筛选和空间转录组学揭示细胞内、细胞间和功能转录 circuit
Loϊc Binan,Aiping Jiang,Serwah A Danquah et al.
Loϊc Binan et al.
Pooled optical screens have enabled the study of cellular interactions, morphology, or dynamics at massive scale, but they have not yet leveraged the power of highly plexed single-cell resolved transcriptomic readouts to inform molecular pa...
Crop root bacterial and viral genomes reveal unexplored species and microbiome patterns [0.03%]
作物根部的细菌和病毒基因组揭示了未被发现的物种和微生物组特征
Rui Dai,Jingying Zhang,Fang Liu et al.
Rui Dai et al.
Reference genomes of root microbes are essential for metagenomic analyses and mechanistic studies of crop root microbiomes. By combining high-throughput bacterial cultivation with metagenomic sequencing, we constructed comprehensive bacteri...
Polarity-guided uneven mitotic divisions control brassinosteroid activity in proliferating plant root cells [0.03%]
极性指导的不对称有丝分裂控制增殖植物根细胞中的类固醇活性
Nemanja Vukašinović,Che-Wei Hsu,Marco Marconi et al.
Nemanja Vukašinović et al.
Brassinosteroid hormones are positive regulators of plant organ growth, yet their function in proliferating tissues remains unclear. Here, through integrating single-cell RNA sequencing with long-term live-cell imaging of the Arabidopsis ro...
Genome-scale resources in the infant gut symbiont Bifidobacterium breve reveal genetic determinants of colonization and host-microbe interactions [0.03%]
婴儿肠道共生菌布氏嗜粘短链细菌的基因组规模资源揭示了定植和宿主-微生物相互作用的遗传决定因素
Anthony L Shiver,Jiawei Sun,Rebecca Culver et al.
Anthony L Shiver et al.
Bifidobacteria represent a dominant constituent of human gut microbiomes during infancy, influencing nutrition, immune development, and resistance to infection. Despite interest in bifidobacteria as a live biotic therapy, our understanding ...
Engineering mtDNA deletions by reconstituting end joining in human mitochondria [0.03%]
在人类线粒体中通过重建末端连接来工程化mtDNA缺失
Yi Fu,Max Land,Tamar Kavlashvili et al.
Yi Fu et al.
Recent breakthroughs in the genetic manipulation of mitochondrial DNA (mtDNA) have enabled precise base substitutions and the efficient elimination of genomes carrying pathogenic mutations. However, reconstituting mtDNA deletions linked to ...
Tianxiong Yu,Michaela B J Blyton,Milky Abajorga et al.
Tianxiong Yu et al.
Koala retrovirus-A (KoRV-A) is spreading through wild koalas in a north-to-south wave while transducing the germ line, modifying the inherited genome as it transitions to an endogenous retrovirus. Previously, we found that KoRV-A is express...
Functional diversification of dietary plant small molecules by the gut microbiome [0.03%]
肠道微生物对饮食中植物源小分子的功能性分化
Gavin A Kuziel,Gabriel L Lozano,Corina Simian et al.
Gavin A Kuziel et al.
Plants are composed of diverse secondary metabolites (PSMs), which are widely associated with human health. Whether and how the gut microbiome mediates such impacts of PSMs is poorly understood. Here, we show that discrete dietary and medic...
Han Xu,Chengchi Fang,Wenjie Xu et al.
Han Xu et al.
The deep sea, especially hadal zones, characterized by high-hydrostatic pressure, low temperatures, and near-total darkness, present some of the most challenging environments for life on Earth. However, teleost fish have successfully coloni...
The amphipod genome reveals population dynamics and adaptations to hadal environment [0.03%]
对虾基因组揭示了种群动态和对深海环境的适应性
Haibin Zhang,Shuai Sun,Jun Liu et al.
Haibin Zhang et al.
The amphipod Hirondellea gigas is a dominant species inhabiting the deepest part of the ocean (∼6,800-11,000 m), but little is known about its genetic adaptation and population dynamics. Here, we present a chromosome-level genome of H. gig...
Microbial ecosystems and ecological driving forces in the deepest ocean sediments [0.03%]
最深海洋沉积物中的微生物生态系统及其生态驱动因素
Xiang Xiao,Weishu Zhao,Zewei Song et al.
Xiang Xiao et al.
Systematic exploration of the hadal zone, Earth's deepest oceanic realm, has historically faced technical limitations. Here, we collected 1,648 sediment samples at 6-11 km in the Mariana Trench, Yap Trench, and Philippine Basin for the Mari...