Structure and engineering of the large serine recombinase Bxb1 for gene integration [0.03%]
巨大丝氨酸重组酶Bxb1的结构和工程改造以整合基因
Teppei Soma,Masahiro Hiraizumi,Christopher W Fell et al.
Teppei Soma et al.
The large serine recombinase Bxb1 catalyzes recombination between DNA molecules containing compatible attP and attB sequences, offering broad applications in genome engineering and gene therapies. Here, we present cryo-electron microscopy s...
Tyler Rhinesmith,Anna Albecka,Marina Vaysburd et al.
Tyler Rhinesmith et al.
TRIM21 is an exceptionally versatile ubiquitin ligase that can be directed by antibodies to target oligomeric protein scaffolds, viral capsids, and proteopathic aggregates for intracellular degradation. How the cell degrades these typically...
Tim Pflästerer,Andrea Dlasková,Stuart Caldwell et al.
Tim Pflästerer et al.
Like mitochondria themselves, research on this organelle can take many shapes and sizes. This month, to coincide with the Cell Press Symposia: Multifaceted Mitochondria, we are highlighting the diversity of the global mitochondria community...
Use it or lose it: A ubiquitin-mediated autophagy pathway for degradation of MHC-I in pancreatic cancer [0.03%]
用进废退：一种泛素介导的自噬途径用于胰腺癌中MHC-I的降解
Simon Wilkinson
Simon Wilkinson
In this issue of Molecular Cell, Berquez et al.1 reveal that MHC-I is degraded from within the endoplasmic reticulum (ER) by a ubiquitin-driven autophagy pathway involving co-operation of a cytoplasmic and an ER-phagy cargo receptor. ...
RNAP at the apex: Cryo-ET unveils the torsional tango between plasmid DNA and double-helix-melting proteins [0.03%]
RNA聚合酶处于顶点：Cryo-ET揭示了质粒DNA与双链熔解蛋白之间的扭曲探戈舞步
Zhaoqian Wang,Yihan Wan
Zhaoqian Wang
In this issue, Zhang and Cañari-Chumpitaz et al.1 find that RNA polymerase (RNAP) preferentially binds to the apices of supercoiled DNA plectonemes, which facilitates promoter escape but imposes a torsional trade-off during elongation. In ...
Everything you always wanted to know about plasmid chromatinization … but were afraid to ask [0.03%]
关于染色质化……你想知道的一切（关于质粒）
Christopher Benner,Sven Heinz
Christopher Benner
In this issue of Molecular Cell, Mallory et al.1 use single-molecule DNA methyltransferase footprinting to provide an unprecedented view of the chromatin and transcriptional state of reporter plasmid molecules in human cells. ...
Reductive death is averted by a conserved de novo lipogenic switch [0.03%]
由一种保守的从头脂质生物合成转换控制的非氧化性细胞死亡机制
Fasih M Ahsan,Jen F Rotti,Armen I Yerevanian et al.
Fasih M Ahsan et al.
Biguanides, including metformin, the world's most prescribed oral hypoglycemic, extend health span and lifespan in vertebrates and invertebrates. Given the widespread use and apparent safety of metformin, it is assumed that its effects are ...
The heat shock transcription factor, HSF1, stimulates the catalytic engagement of topoisomerase IIβ over topoisomerase IIα [0.03%]
热休克转录因子HSF1可通过刺激拓扑异构酶IIβ的催化而刺激拓扑异构酶IIα
Lahiri Konada,Thomas E Catley,Lance Heady et al.
Lahiri Konada et al.
Topoisomerase II (TOP2) poisons, such as etoposide, are potent antineoplastic drugs that also cause significant secondary toxicity to postmitotic cells. Proliferating mammalian cells express two TOP2 isoforms, TOP2A and TOP2B, whereas postm...
IS110 transposon utilizes two mechanistically distinct RNA-guided transposition pathways [0.03%]
IS110转座子利用两种机制上不同的RNA引导的转座途径
Xuanlong Sun,Chen Yang,Zhaohui Cai et al.
Xuanlong Sun et al.
The IS110 elements employ RNA-guided transposases using bridge-RNA to coordinate donor and target DNA recognition and integration via donor- and target-binding loops, which offers promise for DSB-free genome editing. However, their transpos...
Deacetylated PCBP1 licenses PARP1 activity for DNA damage repair [0.03%]
脱乙酰化PCBP1授权PARP1活性以进行DNA损伤修复
Yuxin Shu,Jun Zhang,Linmin Zhou et al.
Yuxin Shu et al.
Poly (ADP-ribose) polymerase 1 (PARP1) is a DNA damage sensor and one of the initiating enzymes essential for DNA repair. Understanding how to control PARP1 activity is critical for developing strategies to maintain genome stability. Here, ...