Metabolic Engineering Strategies for Flavonoid Biosynthesis in Saccharomyces cerevisiae: Current Advances and Future Perspectives [0.03%]
用于酿酒酵母黄酮生物合成的代谢工程策略：当前进展和未来展望
Xu Cheng,Mengnan Han,Guogeng Zhang et al.
Xu Cheng et al.
Flavonoids are a class of plant secondary metabolites with diverse physiological activities, including antioxidant and anti-inflammatory effects. Growing demand for these compounds now exceeds the capacity of traditional plant extraction me...
"Bioprinting of Hair: How Far We Proceeded and What Needs to be Done" [0.03%]
hair的生物打印：我们已经走了多远以及我们需要做什么
Mamata Mishra,Rinky Kapoor,Sunil Manohar Keswani et al.
Mamata Mishra et al.
Alopecia is a common dermatologic disorder with psychological and quality-of-life impact. Current options such as PRP, topical agents, microneedling or dermarollers, drugs, and transplantation, often provide only partial or short-term benef...
Numerical and Experimental Assessment of Oxygen Distribution in a Perfusion Bioreactor to Mimic the Osteochondral Niche [0.03%]
数值和实验评估仿生骨软骨微环境的灌注生物反应器中的氧分布
Franziska Alt,Eric Langner,Hans-Peter Wiesmann et al.
Franziska Alt et al.
Oxygen supply is a critical parameter in 3D cell cultivation using bioreactors. Since bioreactor designs often prioritise practical constraints, understanding the oxygen supply dynamics of the media is crucial for achieving either uniform o...
IMACulate(DE3), an E. coli Strain for High Purity His-Tagged Protein Purifications [0.03%]
一种用于高纯度组氨酸标记蛋白质分离的大肠杆菌菌株IMACulate (DE3)
Jia Q Truong,Dingyi Yu,Nan Hao et al.
Jia Q Truong et al.
Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his-tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co-purifi...
Microbial Inoculant-Driven Degradation of Microplastics Associated With Garbage Environment and Assessment of Degradation Product Toxicity [0.03%]
与垃圾环境相关的微塑料的微生物促分解及对分解产物毒性的评估
G P Avinash,S Karthick Raja Namasivayam
G P Avinash
This investigation revealed that the application of specific microbial inoculants could facilitate the effective biodegradation of polyethylene terephthalate (PET) microplastics collected from urban garbage sites, resulting in non-toxic end...
Resilient High-Rate Sulfidogenesis in a Hydrogen-Based Membrane Biofilm Reactor: Mechanistic Analysis of Kinetic-Limited Performance Under Variable Loading [0.03%]
基于氢气的膜生物反应器中耐受型高效硫酸盐还原作用：不同负荷下动力学限制型性能的作用机理分析
José Suárez,Sebastián Roa,Vicente Valdés et al.
José Suárez et al.
Copper mining industry generates water with high sulfate (SO4 2-) concentrations. The hydrogen-based membrane biofilm reactor (H2-MBfR) is a promising sulfidogenesis solution, using H2 as a clean electron donor. An H2-MBfR was operated for ...
Efficient Synthesis of Ergothioneine in Escherichia coli Through Identification of Bacterial-Type Egt1 and Self-Assemble of Pathway Enzymes [0.03%]
基于细菌型Egt1的高效合成及 ergothioneine途径酶体外自组装研究
Xin-Yi Chen,Shuai Yan,Hang-Cheng Li et al.
Xin-Yi Chen et al.
Ergothioneine (EGT) offers diverse physiological benefits and potential applications. Production of EGT by engineered bacterial strain has emerged as an eco-friendly and cost-effective alternative to traditional extraction and chemical synt...
Machine Learning Models of Phase Contrast Images Predict Efficiency of Human Pluripotent Stem Cell Differentiation to Cardiomyocytes [0.03%]
机器学习模型预测人多能干细胞分化为心肌细胞的效率
Austin K Feeney,Yaniv Ovadia,Aaron D Simmons et al.
Austin K Feeney et al.
Terminal cell types derived from human pluripotent stem cells (hPSCs) are at the forefront of emerging cell and gene therapy products. hPSC-derived cardiomyocytes (hPSC-CMs) are of particular interest in understanding and treating heart dis...
Engineering a Novel Cofactor-Versatile Short-Chain Dehydrogenase for Efficient Synthesis of Chiral Alcohols [0.03%]
工程化一种新型辅因子通用性短链脱氢酶高效合成手性醇化合物
Li-Li Yao,Xian-Heng Song,Kai-Cheng Huang et al.
Li-Li Yao et al.
Short-chain dehydrogenases/reductases (SDRs) are widely used for the asymmetric synthesis of chiral alcohols, which are critical building blocks in pharmaceutical manufacturing. In this study, a novel SDR from Bacillus subtilis (BsSDR) was ...
On the Prediction of pDNA Productivity Across Diverse Bioprocesses Using Ensemble Hybrid Models [0.03%]
基于集成混合模型的预测多样性生物工艺中pDNA产量的研究
Nikolaos Stratis,Massimo Morbidelli,Alexandros Kiparissides
Nikolaos Stratis
The global demand for plasmid DNA (pDNA) is rapidly increasing due to its key role in the manufacture of mRNA vaccines and advanced therapy medicinal products (ATMPs). However, the reliance on suboptimal batch and/or fed-batch processes, as...